在发育中的斑马鱼肝细胞中Erk信号动力学的实时成像

IF 2.5 3区生物学 Q2 DEVELOPMENTAL BIOLOGY

Developmental biology Pub Date : 2025-04-12 DOI:10.1016/j.ydbio.2025.04.009

Faraz Ahmed Butt , Alessandro De Simone , Stefano Di Talia , Kenneth D. Poss

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引用次数: 0

摘要

在器官发育过程中，区域和组织范围的信号通路调控协调细胞增殖和分化。在这项研究中，我们建立了一个纵向分析斑马鱼肝脏发育的成像平台。我们为细胞外信号调节激酶（Erk）和c-Jun n -末端激酶（Jnk）信号的激酶易位报告者生成了肝细胞特异性转基因细胞系，并通过这些细胞系捕获了控制肝细胞快速扩增的信号动力学，从而在单细胞分辨率下产生功能肝脏。我们的研究结果揭示了Erk信号在肝脏发育过程中的波动，并介绍了在器官形态发生过程中研究细胞类型特异性信号动力学的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

In toto live imaging of Erk signaling dynamics in developing zebrafish hepatocytes

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In toto live imaging of Erk signaling dynamics in developing zebrafish hepatocytes

Regional and tissue-wide regulation of signaling pathways orchestrates cellular proliferation and differentiation during organ development. In this study, we established an imaging platform for longitudinal analysis of liver development in live developing zebrafish. We generated hepatocyte-specific transgenic lines for kinase translocation reporters of extracellular signal-regulated kinase (Erk) and c-Jun N-terminal kinase (Jnk) signaling, and with these we captured signaling dynamics that govern rapid expansion of hepatocytes toward creation of the functioning liver at single-cell resolution. Our findings reveal Erk signaling fluctuations as the liver develops and introduce methodology for investigating cell-type specific signaling dynamics during organ morphogenesis.

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来源期刊

Developmental biology 生物-发育生物学

CiteScore

5.30

自引率

3.70%

发文量

182

审稿时长

1.5 months

期刊介绍： Developmental Biology (DB) publishes original research on mechanisms of development, differentiation, and growth in animals and plants at the molecular, cellular, genetic and evolutionary levels. Areas of particular emphasis include transcriptional control mechanisms, embryonic patterning, cell-cell interactions, growth factors and signal transduction, and regulatory hierarchies in developing plants and animals.