一种基于供体-受体共价三嗪框架的“开-关-开”电化学发光配体传感器在缺口内切酶驱动的DNA行走机辅助下检测黄曲霉毒素B1

IF 3.9 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Langmuir Pub Date : 2025-04-15 DOI:10.1021/acs.langmuir.5c00207

Chuanpan Guo, Yifei Wang, Zhenzhen Li, Yuqi Guo, Fenghe Duan, Linghao He, Shuai Zhang, Minghua Wang, Zhihong Zhang

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引用次数: 0

摘要

构建了一种基于供体-受体（D-A）共轭共价三嗪框架（CTF）的“开关”信号可切换电化学发光（ECL）适体传感器，在核酸切酶（Nb.BbvCI）驱动的DNA行走机的辅助下，对黄曲霉毒素B1 （AFB1）进行灵敏、准确的检测。D-A共轭CTF由2,4,6-三（4-甲苯基）-1,3,5-三嗪（TFPT）和三（4-氨基苯基）甲烷（TAPM）（标记为TFPT-TAPM-CTF）反应生成，同时作为优良的ECL发射器和锚定生物探针的平台。tfpt - ttap - ctf的高ECL响应可以通过锚定的cy5标记单链DNA （Cy5-ssDNA）通过ECL共振能量转移来抑制。此外，在afb1靶向适体和DNA行体（Hp）之间产生的双链DNA的固定化降低了tfpt - ttap - ctf的ECL反应。当检测AFB1时，适体从双链DNA中分离以捕获特定靶标，导致自由Hp链与Cy5-ssDNA杂交。在Nb的帮助下。BbvCI释放的部分Cy5-ssDNA在一定程度上有助于恢复tfpt - ttap - ctf的ECL应答，进一步解放Hp链，使其自主结合到另一个Cy5-ssDNA上，触发新的切割过程。在1.0 pg·mL-1 ~ 5.0 × 104 pg·mL-1范围内，ECL感应传感器的检测限为0.59 pg·mL-1，具有较高的选择性和良好的实用性。本工作拓宽了CTF在食品安全领域的应用，为食品中真菌毒素的灵敏、精确检测提供了一种新的检测策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A Donor–Acceptor Covalent Triazine Framework-based “On–Off–On” Electrochemiluminescence Aptasensor for Detecting Aflatoxin B1 with the Assistance of Nicking Endonuclease-powered DNA Walking Machine

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A designed “on–off–on” signal-switchable electrochemiluminescence (ECL) aptasensor based on a donor–acceptor (D–A) conjugated covalent triazine framework (CTF) has been constructed for the sensitive and accurate detection of aflatoxin B1 (AFB1) with the assistance of a nicking endonuclease (Nb.BbvCI)-powered DNA walking machine. The D–A conjugated CTF, generated from the reaction between 2,4,6-tris(4-formylphenyl)-1,3,5-triazine (TFPT) and tris(4-aminophenyl)methane (TAPM) (denoted as TFPT-TAPM-CTF), simultaneously serves as a superior ECL emitter and a platform for anchoring the bioprobe. The high ECL response of TFPT-TAPM-CTF can be quenched by the anchored Cy5-labeled single-strand DNA (Cy5-ssDNA) via ECL resonance energy transfer. Furthermore, the immobilization of the double-strand DNA generated between the AFB1-targeting aptamer and the DNA walker (Hp) reduces the ECL response of TFPT-TAPM-CTF. When detecting AFB1, the aptamer separates from the double-strand DNA to capture specific targets, resulting in the hybridization of the free Hp strand and Cy5-ssDNA. With the assistance of Nb.BbvCI, the released partial Cy5-ssDNA helps recover the ECL response of TFPT-TAPM-CTF to some extent, further liberating the Hp strand to autonomously bind to another Cy5-ssDNA and trigger a new cleavage process. In addition to its high selectivity and promising practicality, the constructed ECL aptasensor shows an ultralow detection limit of 0.59 pg·mL^–1 within a wide range from 1.0 pg·mL^–1 to 5.0 × 10⁴ pg·mL^–1. This work broadens the application of CTF in the food safety field and provides a new aptasensing strategy for the sensitive and precise inspection of mycotoxins in food products.

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来源期刊

Langmuir 化学-材料科学：综合

CiteScore

6.50

自引率

10.30%

发文量

1464

审稿时长

2.1 months

期刊介绍： Langmuir is an interdisciplinary journal publishing articles in the following subject categories: Colloids: surfactants and self-assembly, dispersions, emulsions, foams Interfaces: adsorption, reactions, films, forces Biological Interfaces: biocolloids, biomolecular and biomimetic materials Materials: nano- and mesostructured materials, polymers, gels, liquid crystals Electrochemistry: interfacial charge transfer, charge transport, electrocatalysis, electrokinetic phenomena, bioelectrochemistry Devices and Applications: sensors, fluidics, patterning, catalysis, photonic crystals However, when high-impact, original work is submitted that does not fit within the above categories, decisions to accept or decline such papers will be based on one criteria: What Would Irving Do? Langmuir ranks #2 in citations out of 136 journals in the category of Physical Chemistry with 113,157 total citations. The journal received an Impact Factor of 4.384*. This journal is also indexed in the categories of Materials Science (ranked #1) and Multidisciplinary Chemistry (ranked #5).