手性传感结合核酸酶活性测定跟踪溶液中Cas9的动态：ROA和CPL研究

IF 4.3 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemical Communications Pub Date : 2025-04-15 DOI:10.1039/d5cc00971e

Monika Halat, Magdalena Klimek-Chodacka, Agnieszka Domagała, Grzegorz Zając, Tomasz Oleszkiewicz, Josef Kapitán, Rafal Baranski

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引用次数: 0

摘要

对SpyCas9蛋白的热带研究极为罕见。需要非破坏性的方法来表征其活性核糖核蛋白形式。利用拉曼光学活性（ROA）和圆偏振发光（CPL），我们提出了一种新的方法来检测参与CRISPR-Cas技术的关键生物分子，同时保留其原始的核分解活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Chiral sensing combined with nuclease activity assay to track Cas9 dynamics in solution: ROA and CPL study

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Chiral sensing combined with nuclease activity assay to track Cas9 dynamics in solution: ROA and CPL study

Chiroptical studies of the SpyCas9 protein are extremely rare. Nondestructive methods are needed to characterize its active ribonucleoprotein form. Using Raman optical activity (ROA) and circularly polarized luminescence (CPL), we present a new approach to detect key biomolecules involved in CRISPR-Cas technology while preserving their original nucleolytic activity.

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来源期刊

Chemical Communications 化学-化学综合

CiteScore

8.60

自引率

4.10%

发文量

2705

审稿时长

1.4 months

期刊介绍： ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.