Amina Rhouati, Rawan Ramadan Mohamed, Madhurya Chandel, Karamullah Eisawi, Michael Naguib, Agnieszka Jastrzębska and Mohammed Zourob
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引用次数: 0
摘要
纳米层合材料以其优越的物理化学性能受到了广泛的关注。mxene型材料在生物传感器领域具有广阔的应用前景。然而,MBenes在这一领域还没有得到广泛的研究。因此,在此,我们比较了纳米层合MAB相和ML (Multilayer) MBene相的性能,并将这些相用于电化学适体感应平台的开发。微阵列平台由两个工作碳电极组成,分别用金纳米粒子(AuNPs)和多壁碳纳米管(MWCNTs)修饰。通过修饰电极,比较了ML - MBene与其亲本MAB相的效率。由于其良好的电化学效果,MBene被进一步选择用于电感传感器。MBene/AuNPs/ mwcnt修饰电极被11-脱氧皮质醇(11-DCL)特异性适配体功能化。该传感器对11-DCL的检出限为0.014 pg mL - 1,检测范围为0.01 ~ 100 pg mL - 1。此外,通过对真实血清样品的验证,该适体传感器对干扰物具有良好的选择性和适用性。
Electrochemical aptasensing platform based on nanolaminated MAB/MBene phases for the efficient detection of 11-deoxycortisol†
Nanolaminated materials have attracted much attention because of their advantageous physicochemical properties. MXene-type materials have shown great promise in the field of biosensors. However, MBenes have not been extensively explored in this field. Therefore, herein, we compared the properties of nanolaminated MAB phases and ML (Multilayer) MBene, and these phases were used in the development of an electrochemical aptasensing platform. The microarray platform comprised two working carbon electrodes, which were modified with gold nanoparticles (AuNPs) and multi-walled carbon nanotubes (MWCNTs). The efficiency of ML MBene was compared with its parental MAB phase by modifying the electrodes. Owing to its better electrochemical results, MBene was further selected for application in an aptasensor. MBene/AuNPs/MWCNT-modified electrode was functionalized with an aptamer specific to the hormone 11-deoxycortisol (11-DCL). The resulting aptasensor showed an outstanding limit of detection of 0.014 pg mL−1 for 11-DCL, in relation to the determination range of 0.01 to 100 pg mL−1. In addition, the aptasensor showed excellent selectivity towards interfering agents and good applicability by validating the results of real serum samples.