Juan Chai, Yaxin Hou, Chenxiao Hou, Zhenghong Guo and Juan Li
{"title":"uox掺杂Cu2O@HP-HKUST-1快速合成用于尿酸敏感比色法检测†","authors":"Juan Chai, Yaxin Hou, Chenxiao Hou, Zhenghong Guo and Juan Li","doi":"10.1039/D4NJ04912H","DOIUrl":null,"url":null,"abstract":"<p >Uric acid (UA) is the final catabolism product of purines, which widely exist in various body fluids, including serum and urine. The accurate and sensitive detection of UA is essential for health monitoring and diagnosing many diseases. Herein, a hybrid structure urate oxidase (UOx)-doped Cu<small><sub>2</sub></small>O@HP-HKUST-1 material was proposed for colorimetric and ratiometric detection of UA. In the presence of UOx, the decomposition of UA can produce H<small><sub>2</sub></small>O<small><sub>2</sub></small>, which was then catalyzed by Cu<small><sub>2</sub></small>O@HP-HKUST-1 into ˙OH radicals. The ˙OH radicals could then oxidize 3,3′,5,5′-tetramethylbenzidine (TMB), as evidenced by a UV-vis absorption peak at 652 nm. Finally, a biosensor based on UOx-Cu<small><sub>2</sub></small>O@HP-HKUST-1 was employed to determine UA levels in urine samples. The linear range was 5–600 μM and the limit of detection for colorimetric detection of UA was 2.58 μM.</p>","PeriodicalId":95,"journal":{"name":"New Journal of Chemistry","volume":" 16","pages":" 6761-6767"},"PeriodicalIF":2.5000,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Facile synthesis of UOx-doped Cu2O@HP-HKUST-1 for sensitive colorimetric detection of uric acid†\",\"authors\":\"Juan Chai, Yaxin Hou, Chenxiao Hou, Zhenghong Guo and Juan Li\",\"doi\":\"10.1039/D4NJ04912H\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Uric acid (UA) is the final catabolism product of purines, which widely exist in various body fluids, including serum and urine. The accurate and sensitive detection of UA is essential for health monitoring and diagnosing many diseases. Herein, a hybrid structure urate oxidase (UOx)-doped Cu<small><sub>2</sub></small>O@HP-HKUST-1 material was proposed for colorimetric and ratiometric detection of UA. In the presence of UOx, the decomposition of UA can produce H<small><sub>2</sub></small>O<small><sub>2</sub></small>, which was then catalyzed by Cu<small><sub>2</sub></small>O@HP-HKUST-1 into ˙OH radicals. The ˙OH radicals could then oxidize 3,3′,5,5′-tetramethylbenzidine (TMB), as evidenced by a UV-vis absorption peak at 652 nm. Finally, a biosensor based on UOx-Cu<small><sub>2</sub></small>O@HP-HKUST-1 was employed to determine UA levels in urine samples. The linear range was 5–600 μM and the limit of detection for colorimetric detection of UA was 2.58 μM.</p>\",\"PeriodicalId\":95,\"journal\":{\"name\":\"New Journal of Chemistry\",\"volume\":\" 16\",\"pages\":\" 6761-6767\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-03-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"New Journal of Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2025/nj/d4nj04912h\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"New Journal of Chemistry","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/nj/d4nj04912h","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Facile synthesis of UOx-doped Cu2O@HP-HKUST-1 for sensitive colorimetric detection of uric acid†
Uric acid (UA) is the final catabolism product of purines, which widely exist in various body fluids, including serum and urine. The accurate and sensitive detection of UA is essential for health monitoring and diagnosing many diseases. Herein, a hybrid structure urate oxidase (UOx)-doped Cu2O@HP-HKUST-1 material was proposed for colorimetric and ratiometric detection of UA. In the presence of UOx, the decomposition of UA can produce H2O2, which was then catalyzed by Cu2O@HP-HKUST-1 into ˙OH radicals. The ˙OH radicals could then oxidize 3,3′,5,5′-tetramethylbenzidine (TMB), as evidenced by a UV-vis absorption peak at 652 nm. Finally, a biosensor based on UOx-Cu2O@HP-HKUST-1 was employed to determine UA levels in urine samples. The linear range was 5–600 μM and the limit of detection for colorimetric detection of UA was 2.58 μM.
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