高频重复经颅磁刺激可增强缺血性中风大鼠模型的白质完整性：使用基于束的空间统计的弥散张量成像研究

IF 4.7 2区医学 Q1 NEUROIMAGING

NeuroImage Pub Date : 2025-04-11 DOI:10.1016/j.neuroimage.2025.121204

Jiemei Chen , Chao Li , Jiena Hong , Fei Zhao , Jiantao Zhang , Man Yang , Shengxiang Liang , Hongmei Wen

{"title":"高频重复经颅磁刺激可增强缺血性中风大鼠模型的白质完整性：使用基于束的空间统计的弥散张量成像研究","authors":"Jiemei Chen , Chao Li , Jiena Hong , Fei Zhao , Jiantao Zhang , Man Yang , Shengxiang Liang , Hongmei Wen","doi":"10.1016/j.neuroimage.2025.121204","DOIUrl":null,"url":null,"abstract":"<div><div>Ischemic stroke leads to white matter damage and neurological deficits. Previous studies have revealed that high-frequency repetitive transcranial magnetic stimulation (HF-rTMS) has beneficial effects on white matter reorganization and neurological recovery after stroke. However, the characteristics of poststroke white matter repair after treatment with HF-rTMS remain unclear. Therefore, this study used diffusion tensor imaging (DTI) to investigate the impact of HF-rTMS on white matter integrity following middle cerebral artery occlusion (MCAO) in a rat model. The modified neurological severity score (mNSS) and T2-weighted imaging data were used to assess neurological function and infarct size. We used a tract-based spatial statistics (TBSS) approach to analyze changes in fractional anisotropy (FA) across various white matter tracts. Furthermore, we performed Luxol fast blue (LFB) staining and transmission electron microscopy (TEM) to detect white matter and myelin damage. The results revealed that compared with the tMCAO group, the tMCAO+rTMS group presented a significant decrease in infarct size and the mNSS, as well as significantly greater FA values, mostly in the left external capsule, left internal capsule, left optic tract, left deep cerebral white matter, left stria terminalis and right external capsule. The LFB staining and electron microscopy results are consistent with the DTI results. These findings suggest that HF-rTMS contributes to the recovery of white matter integrity and neurological function. This study underscores the importance of HF-rTMS as a noninvasive intervention for enhancing poststroke neurological recovery by improving white matter integrity.</div></div>","PeriodicalId":19299,"journal":{"name":"NeuroImage","volume":"311 ","pages":"Article 121204"},"PeriodicalIF":4.7000,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"High-Frequency repetitive transcranial magnetic stimulation enhances white matter integrity in a rat model of ischemic stroke: A diffusion tensor imaging study using tract-based spatial statistics\",\"authors\":\"Jiemei Chen , Chao Li , Jiena Hong , Fei Zhao , Jiantao Zhang , Man Yang , Shengxiang Liang , Hongmei Wen\",\"doi\":\"10.1016/j.neuroimage.2025.121204\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Ischemic stroke leads to white matter damage and neurological deficits. Previous studies have revealed that high-frequency repetitive transcranial magnetic stimulation (HF-rTMS) has beneficial effects on white matter reorganization and neurological recovery after stroke. However, the characteristics of poststroke white matter repair after treatment with HF-rTMS remain unclear. Therefore, this study used diffusion tensor imaging (DTI) to investigate the impact of HF-rTMS on white matter integrity following middle cerebral artery occlusion (MCAO) in a rat model. The modified neurological severity score (mNSS) and T2-weighted imaging data were used to assess neurological function and infarct size. We used a tract-based spatial statistics (TBSS) approach to analyze changes in fractional anisotropy (FA) across various white matter tracts. Furthermore, we performed Luxol fast blue (LFB) staining and transmission electron microscopy (TEM) to detect white matter and myelin damage. The results revealed that compared with the tMCAO group, the tMCAO+rTMS group presented a significant decrease in infarct size and the mNSS, as well as significantly greater FA values, mostly in the left external capsule, left internal capsule, left optic tract, left deep cerebral white matter, left stria terminalis and right external capsule. The LFB staining and electron microscopy results are consistent with the DTI results. These findings suggest that HF-rTMS contributes to the recovery of white matter integrity and neurological function. This study underscores the importance of HF-rTMS as a noninvasive intervention for enhancing poststroke neurological recovery by improving white matter integrity.</div></div>\",\"PeriodicalId\":19299,\"journal\":{\"name\":\"NeuroImage\",\"volume\":\"311 \",\"pages\":\"Article 121204\"},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2025-04-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"NeuroImage\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1053811925002071\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"NEUROIMAGING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"NeuroImage","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1053811925002071","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"NEUROIMAGING","Score":null,"Total":0}

引用次数: 0

摘要

缺血性脑卒中会导致白质损伤和神经功能缺损。以往的研究表明，高频重复经颅磁刺激（HF-rTMS）对脑卒中后的白质重组和神经功能恢复有好处。然而，高频经颅磁刺激治疗后脑卒中后白质修复的特征仍不清楚。因此，本研究使用弥散张量成像（DTI）在大鼠模型中研究了高频经颅磁刺激对大脑中动脉闭塞（MCAO）后白质完整性的影响。改良神经严重程度评分（mNSS）和T2加权成像数据用于评估神经功能和梗塞大小。我们使用基于束的空间统计（TBSS）方法分析了不同白质束的分数各向异性（FA）变化。此外，我们还进行了卢克索快蓝（LFB）染色和透射电子显微镜（TEM）检测白质和髓鞘损伤。结果显示，与tMCAO组相比，tMCAO+rTMS组的梗死面积和mNSS显著缩小，FA值显著增大，主要集中在左外囊、左内囊、左视束、左侧大脑深部白质、左侧终纹和右外囊。LFB 染色和电子显微镜结果与 DTI 结果一致。这些研究结果表明，高频经颅磁刺激有助于白质完整性和神经功能的恢复。这项研究强调了高频经颅磁刺激作为一种非侵入性干预措施，通过改善白质完整性来提高中风后神经功能恢复的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

High-Frequency repetitive transcranial magnetic stimulation enhances white matter integrity in a rat model of ischemic stroke: A diffusion tensor imaging study using tract-based spatial statistics

Ischemic stroke leads to white matter damage and neurological deficits. Previous studies have revealed that high-frequency repetitive transcranial magnetic stimulation (HF-rTMS) has beneficial effects on white matter reorganization and neurological recovery after stroke. However, the characteristics of poststroke white matter repair after treatment with HF-rTMS remain unclear. Therefore, this study used diffusion tensor imaging (DTI) to investigate the impact of HF-rTMS on white matter integrity following middle cerebral artery occlusion (MCAO) in a rat model. The modified neurological severity score (mNSS) and T2-weighted imaging data were used to assess neurological function and infarct size. We used a tract-based spatial statistics (TBSS) approach to analyze changes in fractional anisotropy (FA) across various white matter tracts. Furthermore, we performed Luxol fast blue (LFB) staining and transmission electron microscopy (TEM) to detect white matter and myelin damage. The results revealed that compared with the tMCAO group, the tMCAO+rTMS group presented a significant decrease in infarct size and the mNSS, as well as significantly greater FA values, mostly in the left external capsule, left internal capsule, left optic tract, left deep cerebral white matter, left stria terminalis and right external capsule. The LFB staining and electron microscopy results are consistent with the DTI results. These findings suggest that HF-rTMS contributes to the recovery of white matter integrity and neurological function. This study underscores the importance of HF-rTMS as a noninvasive intervention for enhancing poststroke neurological recovery by improving white matter integrity.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

NeuroImage 医学-核医学

CiteScore

11.30

自引率

10.50%

发文量

809

审稿时长

63 days

期刊介绍： NeuroImage, a Journal of Brain Function provides a vehicle for communicating important advances in acquiring, analyzing, and modelling neuroimaging data and in applying these techniques to the study of structure-function and brain-behavior relationships. Though the emphasis is on the macroscopic level of human brain organization, meso-and microscopic neuroimaging across all species will be considered if informative for understanding the aforementioned relationships.