Co3O4纳米片敏化和DNA自反馈循环的原位增强电化学发光血清素检测生物传感器

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-04-08 DOI:10.1016/j.bios.2025.117464

Yongguang Gao , Qiumei Feng , Xiangmin Miao , Yufei Fu

{"title":"Co3O4纳米片敏化和DNA自反馈循环的原位增强电化学发光血清素检测生物传感器","authors":"Yongguang Gao , Qiumei Feng , Xiangmin Miao , Yufei Fu","doi":"10.1016/j.bios.2025.117464","DOIUrl":null,"url":null,"abstract":"<div><div>Here, we described an in situ-boosted electrochemiluminescence (ECL) biosensor for serotonin detection sensitized with the catalysis of Co3O4 nanoplates and self-feedback DNA recycling (SFDR). Specifically, aptamer:S duplex was attached onto the surface of magnetic beads via amide bond. When serotonin presented, it competitively combined with aptamer from aptamer:S duplex. Then, the released S strands reacted with hairpin DNA (H) containing the silenced Mg2+-assisted DNAzyme site in the loop part that immobilized on CdS quantum dots (CdS QDs) modified electrode. The activating Mg2+-assisted DNAzyme cut off H and re-liberated S strands. Therefore, the Mg2+-DNAzyme-aided recycle happened to produce numerous residual single-stranded DNA segments and then reacted with Co3O4-modified P strands on the electrode surface. Such results directly introduced Co3O4 nanoplates close to CdS QDs, resulting in the reduction of H2O2 to •OH and the increase of ECL emission of CdS QDs in CdS QDs-H2O2 system. Based on such signal amplification strategy, a low detection limit of 1.5 pM was obtained for serotonin detection. This approach enabled the proposed ECL biosensor promising for the future application in the trace detection of serotonin.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"281 ","pages":"Article 117464"},"PeriodicalIF":10.7000,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"An in situ-boosted electrochemiluminescence biosensor for serotonin detection sensitized with Co3O4 nanoplates and self-feedback DNA recycling\",\"authors\":\"Yongguang Gao , Qiumei Feng , Xiangmin Miao , Yufei Fu\",\"doi\":\"10.1016/j.bios.2025.117464\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Here, we described an in situ-boosted electrochemiluminescence (ECL) biosensor for serotonin detection sensitized with the catalysis of Co3O4 nanoplates and self-feedback DNA recycling (SFDR). Specifically, aptamer:S duplex was attached onto the surface of magnetic beads via amide bond. When serotonin presented, it competitively combined with aptamer from aptamer:S duplex. Then, the released S strands reacted with hairpin DNA (H) containing the silenced Mg2+-assisted DNAzyme site in the loop part that immobilized on CdS quantum dots (CdS QDs) modified electrode. The activating Mg2+-assisted DNAzyme cut off H and re-liberated S strands. Therefore, the Mg2+-DNAzyme-aided recycle happened to produce numerous residual single-stranded DNA segments and then reacted with Co3O4-modified P strands on the electrode surface. Such results directly introduced Co3O4 nanoplates close to CdS QDs, resulting in the reduction of H2O2 to •OH and the increase of ECL emission of CdS QDs in CdS QDs-H2O2 system. Based on such signal amplification strategy, a low detection limit of 1.5 pM was obtained for serotonin detection. This approach enabled the proposed ECL biosensor promising for the future application in the trace detection of serotonin.</div></div>\",\"PeriodicalId\":259,\"journal\":{\"name\":\"Biosensors and Bioelectronics\",\"volume\":\"281 \",\"pages\":\"Article 117464\"},\"PeriodicalIF\":10.7000,\"publicationDate\":\"2025-04-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biosensors and Bioelectronics\",\"FirstCategoryId\":\"1\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0956566325003380\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOPHYSICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biosensors and Bioelectronics","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0956566325003380","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOPHYSICS","Score":null,"Total":0}

引用次数: 0

摘要

在此，我们介绍了一种原位增强型电化学发光（ECL）生物传感器，该传感器通过 Co3O4 纳米板的催化和自反馈 DNA 循环（SFDR）来敏化血清素检测。具体来说，aptamer:S 双链通过酰胺键连接到磁珠表面。当血清素出现时，它会与 Aptamer:S 双链体中的 Aptamer 竞争性结合。然后，释放出的 S 链与固定在 CdS 量子点（CdS QDs）修饰电极上的发夹 DNA（H）发生反应，发夹 DNA 的环状部分含有沉默的 Mg2+ 辅助 DNA 酶位点。激活的 Mg2+ 辅助 DNA 酶切断了 H 链，重新恢复了 S 链。因此，Mg2+-DNA 酶辅助循环产生了大量残余的单链 DNA 片段，然后与电极表面的 Co3O4 改性 P 链发生反应。这种结果直接将 Co3O4 纳米板引入 CdS QDs 附近，导致 CdS QDs-H2O2 系统中的 H2O2 还原成 -OH 并增加了 CdS QDs 的 ECL 发射。基于这种信号放大策略，血清素检测的检测限低至 1.5 pM。这种方法使得所提出的 ECL 生物传感器有望在未来的血清素痕量检测中得到应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

An in situ-boosted electrochemiluminescence biosensor for serotonin detection sensitized with Co3O4 nanoplates and self-feedback DNA recycling

Here, we described an in situ-boosted electrochemiluminescence (ECL) biosensor for serotonin detection sensitized with the catalysis of Co₃O₄ nanoplates and self-feedback DNA recycling (SFDR). Specifically, aptamer:S duplex was attached onto the surface of magnetic beads via amide bond. When serotonin presented, it competitively combined with aptamer from aptamer:S duplex. Then, the released S strands reacted with hairpin DNA (H) containing the silenced Mg²⁺-assisted DNAzyme site in the loop part that immobilized on CdS quantum dots (CdS QDs) modified electrode. The activating Mg²⁺-assisted DNAzyme cut off H and re-liberated S strands. Therefore, the Mg²⁺-DNAzyme-aided recycle happened to produce numerous residual single-stranded DNA segments and then reacted with Co₃O₄-modified P strands on the electrode surface. Such results directly introduced Co₃O₄ nanoplates close to CdS QDs, resulting in the reduction of H₂O₂ to •OH and the increase of ECL emission of CdS QDs in CdS QDs-H₂O₂ system. Based on such signal amplification strategy, a low detection limit of 1.5 pM was obtained for serotonin detection. This approach enabled the proposed ECL biosensor promising for the future application in the trace detection of serotonin.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.