免疫蛋白组学分析确定了由感染了艾美耳病、新艾美耳病、天牛艾美耳病或尖尾猴艾美耳病的鸡的抗血清识别的具有广泛交叉反应的艾美耳病孢子虫免疫原

IF 2 2区农林科学 Q2 PARASITOLOGY

Veterinary parasitology Pub Date : 2025-04-08 DOI:10.1016/j.vetpar.2025.110462

Haiwei Gong , Haiying Deng , Feng Song , Tao Han , Xiangqin Wang , Shangyu Feng , Weiyi Chen , Liheng Liu

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引用次数: 0

摘要

球虫病是一种广泛存在的原生动物疾病，尽管采取了各种控制措施，包括减毒活疫苗，但仍对全球家禽业构成高风险。为了为下一代具有广泛保护作用的疫苗奠定基础，我们的研究旨在鉴定与超免疫血清交叉反应的孢子子免疫原，以对抗最大艾美耳球虫、柔嫩芽胞杆菌、necatrie和acervulina。总的来说，2D电泳、免疫印迹和MALDI-TOF-MS/MS共发现了12种感兴趣的免疫原性蛋白，其中11种氨基酸序列与NCBI数据库中非冗余蛋白的氨基酸序列相匹配。生物信息学分析表明，这些蛋白参与蛋白质翻译、修饰和降解、信号转导和调节、细胞结构和运输、代谢调节和RNA结合和加工。这些发现现在为疫苗结构的设计提供了多个可行的靶点。

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Immunoproteomic analyses identify broadly cross-reactive sporozoite immunogens of Eimeria maxima recognized by antisera from chickens infected with E. maxima, E. necatrix, E. tenella or E. acervulina

Coccidiosis is a widespread, protozoan disease that continues to impose a high risk to the global poultry industry despite various control measures, including live, attenuated vaccines. To set the stage for next-generation vaccines that are broadly protective, our study aimed to identify sporozoite immunogens that are cross-reactive with hyper-immune sera against Eimeria maxima, E. tenella, E. necatrix and E.acervulina. In all, 2D electrophoresis, immunoblots and MALDI-TOF-MS/MS revealed 12 immunogenic proteins of interest, including 11 that have amino acid sequences matching those of non-redundant proteins in the NCBI database. Bioinformatics analyses revealed that these proteins are involved in protein translation, modification and degradation, signal transduction and regulation, cell structure and transport, metabolic regulation and RNA binding and processing. These findings now offer multiple feasible targets for the design of vaccine constructs.

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来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.