40个r2r3 - myb的特异性图谱揭示了相似物如何通过同二聚体结合靶向不同的顺式元件

IF 23.7 Q1 MICROBIOLOGY
iMeta Pub Date : 2025-03-05 DOI:10.1002/imt2.70009
Tian Li, Hao Chen, Nana Ma, Dingkun Jiang, Jiacheng Wu, Xinfeng Zhang, Hao Li, Jiaqing Su, Piaojuan Chen, Qing Liu, Yuefeng Guan, Xiaoyue Zhu, Juncheng Lin, Jilin Zhang, Qin Wang, Honghong Guo, Fangjie Zhu
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引用次数: 0

摘要

同源转录因子(TFs)经常识别高度相似的DNA基序。同二聚化可以根据它们不同的二聚体结构来区分它们。本研究通过研究R2R3-MYB tf,我们发现同二聚化也可以直接改变识别的DNA基序来区分相似的tf。通过高通量SELEX,我们分析了40个r2r3 - myb亚家族VIII的特异性景观,并策划了833个motif模型。二聚体模型显示同型二聚体结合引起了AtMYBs特异性的变化。以AtMYB2为例,我们发现同源二聚化修饰了其特异性,使其能够识别与密切相关的CCWAA-box atmyb不同的其他顺式调控序列,这些序列在所有atmyb中是独一无二的。由修饰的AtMYB2二聚体特异性描述的基因组位点在进化中是保守的,并参与AtMYB2特异性的转录激活。总的来说,本研究提供了VIII R2R3-MYBs序列偏好的丰富数据,并提出了一种指导密切相关的tf到各自顺式调控位点的替代机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Specificity landscapes of 40 R2R3-MYBs reveal how paralogs target different cis-elements by homodimeric binding

Specificity landscapes of 40 R2R3-MYBs reveal how paralogs target different cis-elements by homodimeric binding

Paralogous transcription factors (TFs) frequently recognize highly similar DNA motifs. Homodimerization can help distinguish them according to their different dimeric configurations. Here, by studying R2R3-MYB TFs, we show that homodimerization can also directly change the recognized DNA motifs to distinguish between similar TFs. By high-throughput SELEX, we profiled the specificity landscape for 40 R2R3-MYBs of subfamily VIII and curated 833 motif models. The dimeric models show that homodimeric binding has evoked specificity changes for AtMYBs. Focusing on AtMYB2 as an example, we show that homodimerization has modified its specificity and allowed it to recognize additional cis-regulatory sequences that are different from the closely related CCWAA-box AtMYBs and are unique among all AtMYBs. Genomic sites described by the modified dimeric specificities of AtMYB2 are conserved in evolution and involved in AtMYB2-specific transcriptional activation. Collectively, this study provides rich data on sequence preferences of VIII R2R3-MYBs and suggests an alternative mechanism that guides closely related TFs to respective cis-regulatory sites.

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CiteScore
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