{"title":"杜鹃花T2T基因组、泛基因组分析及热应激反应基因","authors":"Xiaojing Wang, Ping Zhou, Xiaoyu Hu, Yun Bai, Chenhao Zhang, Yanhong Fu, Ruirui Huang, Niu Suzhen, Xiaoming Song","doi":"10.1002/imt2.70010","DOIUrl":null,"url":null,"abstract":"<p>This study reports the first high-quality telomere-to-telomere (T2T) <i>Rhododendron liliiflorum</i> genome with 11 chromosomes that are gap free. The 24 telomeres and all 13 centromeres detected in this genome, which reached the highest quality gold level. In addition, other three <i>Rhododendron</i> species were sequenced and assembled to the chromosomal level. Based on 15 <i>Rhododendron</i> genomes, we conducted a pan-genome analysis of genus <i>Rhododendron</i>. Combining the genome and whole transcriptome sequencing, we identified several key genes and miRNAs related to the heat stress, which were further verified by transgenic experiments. Our findings provide rich resources for comparative and functional genomics studies of <i>Rhododendron</i> species.\n\n <figure>\n <div><picture>\n <source></source></picture><p></p>\n </div>\n </figure></p>","PeriodicalId":73342,"journal":{"name":"iMeta","volume":"4 2","pages":""},"PeriodicalIF":23.7000,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/imt2.70010","citationCount":"0","resultStr":"{\"title\":\"T2T genome, pan-genome analysis, and heat stress response genes in Rhododendron species\",\"authors\":\"Xiaojing Wang, Ping Zhou, Xiaoyu Hu, Yun Bai, Chenhao Zhang, Yanhong Fu, Ruirui Huang, Niu Suzhen, Xiaoming Song\",\"doi\":\"10.1002/imt2.70010\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>This study reports the first high-quality telomere-to-telomere (T2T) <i>Rhododendron liliiflorum</i> genome with 11 chromosomes that are gap free. The 24 telomeres and all 13 centromeres detected in this genome, which reached the highest quality gold level. In addition, other three <i>Rhododendron</i> species were sequenced and assembled to the chromosomal level. Based on 15 <i>Rhododendron</i> genomes, we conducted a pan-genome analysis of genus <i>Rhododendron</i>. Combining the genome and whole transcriptome sequencing, we identified several key genes and miRNAs related to the heat stress, which were further verified by transgenic experiments. Our findings provide rich resources for comparative and functional genomics studies of <i>Rhododendron</i> species.\\n\\n <figure>\\n <div><picture>\\n <source></source></picture><p></p>\\n </div>\\n </figure></p>\",\"PeriodicalId\":73342,\"journal\":{\"name\":\"iMeta\",\"volume\":\"4 2\",\"pages\":\"\"},\"PeriodicalIF\":23.7000,\"publicationDate\":\"2025-03-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/imt2.70010\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"iMeta\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/imt2.70010\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"iMeta","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/imt2.70010","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
T2T genome, pan-genome analysis, and heat stress response genes in Rhododendron species
This study reports the first high-quality telomere-to-telomere (T2T) Rhododendron liliiflorum genome with 11 chromosomes that are gap free. The 24 telomeres and all 13 centromeres detected in this genome, which reached the highest quality gold level. In addition, other three Rhododendron species were sequenced and assembled to the chromosomal level. Based on 15 Rhododendron genomes, we conducted a pan-genome analysis of genus Rhododendron. Combining the genome and whole transcriptome sequencing, we identified several key genes and miRNAs related to the heat stress, which were further verified by transgenic experiments. Our findings provide rich resources for comparative and functional genomics studies of Rhododendron species.