基于birc5 - mrna的早期乳腺癌纳米传感器的设计与制造（CeO2-Ru@AuNPs-CNTs）

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2025-04-10 DOI:10.1016/j.bej.2025.109754

Engy M. Madkour , Mohamed Abdel-Hakeem , Sahar A. Fadlallah , Sherif Abdelaziz Ibrahim , Rabeay Y.A. Hassan

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引用次数: 0

摘要

含有5的杆状病毒细胞凋亡重复抑制剂（BIRC5）基因编码存活蛋白，已经引起了大量的研究兴趣。Survivin在包括乳腺癌在内的多种癌症中过度表达，这使得BIRC5成为早期诊断和预后的重要生物标志物。因此，我们设计了一种便携式、一次性的电化学生物传感系统，并将其应用于针对BIRC5-mRNA的乳腺癌敏感诊断。在这方面，金纳米颗粒有助于在含有氧化铈(CeO2)-钌（Ru）@多壁碳纳米管（MWCNTs）的纳米复合层的顶部形成自组装单层交联链（4-氨基噻吩（ATP））。随后，化学固定BIRC5-mRNA互补的单链探针。新设计的birc5 - mrna生物传感器的选择性和灵敏度特性通过全面的分析优化来确定，成功地达到了0.4 ag/mL的检测限。新开发的纳米结构BIRC5 mRNA生物传感器可用于快速和直接测定患者血液样本中的靶向BIRC5 mRNA，而无需当前RNA生物传感器可能需要的任何额外标记步骤。

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Designing and fabrication of BIRC5-mRNA-based biosensor for early breast cancer diagnosis using a nanostructured (CeO2-Ru@AuNPs-CNTs)-lab-on-chip

Baculoviral Inhibitor of Apoptosis Repeat Containing 5 (BIRC5) gene, encoding the protein survivin, has garnered substantial research interest. Survivin is overexpressed in various cancers, including breast cancer, making BIRC5 a crucial biomarker for the early diagnosis and prognosis. Thus, a portable and disposable electrochemical biosensing system was designed and applied for the sensitive diagnosis of breast cancer targeting the BIRC5-mRNA. In this regard, gold nanoparticles assisted the formation of a self-assembling monolayer of the crosslinking chain (4-Aminothiophenol (ATP)) on the top of the nanocomposite layer that contains cerium oxide (CeO₂)-Ruthenium (Ru)@multiwall carbon nanotubes (MWCNTs).Subsequently, the single stranded probe complementary to BIRC5-mRNA was chemically immobilized. Selectivity and sensitivity features of the newly designed BIRC5-mRNA-based biosensor were determined through a full assay optimization, whereas a detection limit of 0.4 ag/mL was successfully achieved. The newly developed nanostructured BIRC5 mRNA biosensor could be applied to provide a rapid and direct determination of the targeting BIRC5 mRNA in the patients’ blood samples without any additional labeling step that may be required in currently held RNA biosensors.

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.