Study on the growth and decline patterns and environmental drivers of pathogens during the stabilization process of simulated landfilling municipal solid waste

Waste and leachate in landfills are substantial reservoirs of pathogens, however information about the risk of pathogen contamination during the stabilization process under different landfill conditions is very limited. In this study, dynamic changes of culturable pathogens, bacteria community, and human bacterial pathogens (HBPs) during the stabilization process under different landfill conditions were investigated, and the environmental drivers were explored. Results showed that total coliforms, Enterococcus, and Staphylococcus aureus were the dominant pathogens detected in waste and leachate samples. During the landfill stabilization process, the concentration of culturable pathogens peaked at the hydrolysis-acidification stage (3.6 × 10⁵ CFU·g⁻¹) in the anaerobic condition, fluctuated from 4.18 × 10⁴ to 5.35 × 10⁵ CFU·g⁻¹ in the anaerobic leachate-recirculation condition, and kept rising (from 4.18 × 10⁴ to 2.12 × 10⁶ CFU·g⁻¹) in the micro-aerobic condition. Moreover, HBPs abundance and diversity in the waste and leachate under micro-aerobic conditions were higher than those under the other two conditions, suggesting a higher risk of pathogen contamination. Sulfate and pH were significantly (p < 0.05) correlated with the composition of bacterial communities and HBPs, likely serving as the major environmental driving factors. Additionally, the interactions between HBPs and functional bacterial groups tended towards cooperative symbiotic relationships, with hydrolytic-acidogenic bacteria promoting the growth and proliferation of most pathogens. These findings will help to understand the changes and environmental drivers of pathogens during landfill stabilization, which will provide a theoretical basis for the risk prevention and control of pathogens in waste disposal.