基于微流控和rpa的肺炎支原体多样本快速检测平台的建立

IF 2.1 4区 医学 Q3 INFECTIOUS DISEASES
Ling Zhang , Cheng-Di Sun , Jiao Luo , Li-Guo Liang , Si-Ming Lu
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引用次数: 0

摘要

肺炎支原体是引起非典型社区获得性肺炎的主要呼吸道病原体。早期快速诊断和及时发现病原体至关重要。RPA是一种新兴的PCR技术,与传统PCR技术相比,它可以在室温下进行扩增,同时结合了PCR技术快速检测和高特异性的优点,能够快速准确地检测病原体。方法与结果本研究建立了基于微流控和RPA技术的肺炎支原体检测平台。RPA技术在15 ~ 20分钟内进行,只有肺炎支原体被显著扩增,灵敏度为10拷贝/μL;微流控技术提供了精益的仪器和方便的交互系统。可一次检测3组阴性和阳性qc及18个临床样本。综上所述,我们建立了基于RPA技术的微流控分子检测操作系统,具有简单、快速、准确、经济等优点,为肺炎支原体的实验室检测提供了一种新的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Establishment of a microfluidic and RPA-based platform for rapid multi-sample detection of Mycoplasma pneumoniae

Background

Mycoplasma pneumoniae (M. pneumoniae) is a major respiratory pathogen causing atypical community-acquired pneumonia. Early and rapid diagnosis and timely detection of pathogens are crucial. RPA is an emerging PCR technology that allows amplification at room temperature compared to conventional PCR technology, while combining the advantages of rapid detection and high specificity of PCR technology, enabling rapid and accurate detection of pathogens.

Methods and results

In this study, we established a platform for detection of M. pneumoniae based on microfluidic and RPA technologies. The RPA technology is performed in 15 to 20 minutes, and only M. pneumoniae is significantly amplified at a sensitivity of 10 copies/μL; microfluidic technology provides lean instrumentation and a convenient interactive system. Three groups negative and positive QCs and 18 clinical samples can be tested in a single run.

Significance

In conclusion, we have established a microfluidic molecular assay operating system based on RPA technology, with the advantages of being simple, rapid, accurate and economical, and providing a new tool for laboratory detection of M. pneumoniae.
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来源期刊
CiteScore
5.30
自引率
3.40%
发文量
149
审稿时长
56 days
期刊介绍: Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.
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