用于肾上腺素传感的聚合物稳定银纳米粒子的光化学合成：电化学，EG-FET和基于微控制器的方法

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2025-04-10 DOI:10.1016/j.bej.2025.109755

Chandan Saha , Pooja Kumari , Lungelo Mgenge , Sarit K. Ghosh , Harishchandra Singh , Kaushik Mallick

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引用次数: 0

摘要

肾上腺素是一种关键的儿茶酚胺，调节交感神经系统，在各种生理过程中发挥重要作用。本研究介绍了各种传感器在肾上腺素检测中的应用，基于聚合物稳定的银纳米颗粒复合催化剂，通过紫外线介导的途径合成。采用透射电镜、x射线衍射、拉曼光谱和x射线光电子能谱技术对金属-聚合物复合体系进行了表征。采用不同的伏安技术研究了在催化剂存在下肾上腺素的电化学氧化还原行为。在多巴胺、尿酸、抗坏血酸等常见生物干扰物存在的情况下，电催化剂对肾上腺素的检测表现出高选择性。采用基于扩展栅极场效应晶体管（EG-FET）的传感技术检测肾上腺素，检测限为0.72 μM，灵敏度为0.049 μA.μM−1 cm−2。利用Arduino Uno R4 Wi-Fi模块开发了一种基于微控制器的便携式传感器，实现了实时监控和远程数据传输。该传感器的灵敏度和选择性使其在临床和研究应用中成为一种有前途的肾上腺素现场检测工具。

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Photochemical synthesis of polymer-stabilized silver nanoparticles for epinephrine sensing: Electrochemical, EG-FET and microcontroller based approaches

Epinephrine, a key catecholamine, regulates the sympathetic nervous system and plays a vital role in various physiological processes. This study presents various sensor applications for epinephrine detection, based on a polymer-stabilized silver nanoparticle composite catalyst, synthesized through a UV-mediated route. The metal-polymer composite system was characterized by transmission electron microscopy, X-ray diffraction, Raman and X-ray photoelectron spectroscopy techniques. The electrochemical redox behavior of epinephrine in the presence of the catalyst was studied using various voltametric and amperometric techniques. The electrocatalyst demonstrated high selectivity towards epinephrine detection in the presence of common biological interfering analytes like dopamine, uric acid and ascorbic acid. A sensing technique based on an extended gate field-effect transistor (EG-FET) was applied for epinephrine detection, achieving a limit of detection of 0.72 μM and a sensitivity of 0.049 μA.μM⁻¹.cm⁻². A microcontroller-based portable sensor was developed using an Arduino Uno R4 Wi-Fi module, enabling real-time monitoring and remote data transmission. The sensitivity and selectivity of the sensor make it a promising tool for on-site epinephrine detection in clinical and research applications.

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.