双功能Fe-CDs@MOF复合纳米酶比例荧光探针检测鱼类黄嘌呤

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-04-09 DOI:10.1016/j.talanta.2025.128108

Zhen Xie , Yuhao Gao , Tongshan Zuo , Chen Shen , Han Jiang , Guanhong Xu , Yao Cen , Jing Yang , Qin Hu , Rui Li , Fangdi Wei

{"title":"双功能Fe-CDs@MOF复合纳米酶比例荧光探针检测鱼类黄嘌呤","authors":"Zhen Xie , Yuhao Gao , Tongshan Zuo , Chen Shen , Han Jiang , Guanhong Xu , Yao Cen , Jing Yang , Qin Hu , Rui Li , Fangdi Wei","doi":"10.1016/j.talanta.2025.128108","DOIUrl":null,"url":null,"abstract":"<div><div>A novel bifunctional Fe-CDs@MOF composite nanozyme was synthesized successfully by loading Fe doped carbon dots (Fe-CDs) onto metal-organic framework (MOF) MIL-101 (Fe). The introduction of Fe-CDs enhanced the peroxidase (POD)-like activity of MOF and endowed it with excellent fluorescence properties. Considering the dual functions of Fe-CDs@MOF, we combined it with <em>o</em>-phenylenediamine (OPD)/xanthine oxidase (XOD) to construct a ratiomeric fluorescence probe for xanthine (XAN) detection. XAN was first oxidized by XOD to produce H<sub>2</sub>O<sub>2</sub>, then Fe-CDs@MOF exerted its POD-like activity to catalyze the reaction between OPD and H<sub>2</sub>O<sub>2</sub> to form 2,3-diaminophenazine (DAP). DAP in turn quenched the fluorescence of Fe-CDs@MOF as a result of internal filtration effect. The ratio of the fluorescence intensity of DAP and the fluorescence intensity of Fe-CDs@MOF indicated the XAN content. The XAN detection range was 1–100 μM with a limit of detection of 0.36 μM. The ratiometric fluorescence probe was utilized to analyze XAN in fish samples, and the results obtained were in good agreement with those assayed by high performance liquid chromatography. The method also had the ability to test the freshness of frozen fish. This study provides a novel approach for the design and application of Fe-CDs@MOF composite nanozyme.</div></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"293 ","pages":"Article 128108"},"PeriodicalIF":5.6000,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bifunctional Fe-CDs@MOF composite nanozyme based ratiometric fluorescent probe for the detection of xanthine in fish\",\"authors\":\"Zhen Xie , Yuhao Gao , Tongshan Zuo , Chen Shen , Han Jiang , Guanhong Xu , Yao Cen , Jing Yang , Qin Hu , Rui Li , Fangdi Wei\",\"doi\":\"10.1016/j.talanta.2025.128108\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>A novel bifunctional Fe-CDs@MOF composite nanozyme was synthesized successfully by loading Fe doped carbon dots (Fe-CDs) onto metal-organic framework (MOF) MIL-101 (Fe). The introduction of Fe-CDs enhanced the peroxidase (POD)-like activity of MOF and endowed it with excellent fluorescence properties. Considering the dual functions of Fe-CDs@MOF, we combined it with <em>o</em>-phenylenediamine (OPD)/xanthine oxidase (XOD) to construct a ratiomeric fluorescence probe for xanthine (XAN) detection. XAN was first oxidized by XOD to produce H<sub>2</sub>O<sub>2</sub>, then Fe-CDs@MOF exerted its POD-like activity to catalyze the reaction between OPD and H<sub>2</sub>O<sub>2</sub> to form 2,3-diaminophenazine (DAP). DAP in turn quenched the fluorescence of Fe-CDs@MOF as a result of internal filtration effect. The ratio of the fluorescence intensity of DAP and the fluorescence intensity of Fe-CDs@MOF indicated the XAN content. The XAN detection range was 1–100 μM with a limit of detection of 0.36 μM. The ratiometric fluorescence probe was utilized to analyze XAN in fish samples, and the results obtained were in good agreement with those assayed by high performance liquid chromatography. The method also had the ability to test the freshness of frozen fish. This study provides a novel approach for the design and application of Fe-CDs@MOF composite nanozyme.</div></div>\",\"PeriodicalId\":435,\"journal\":{\"name\":\"Talanta\",\"volume\":\"293 \",\"pages\":\"Article 128108\"},\"PeriodicalIF\":5.6000,\"publicationDate\":\"2025-04-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Talanta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0039914025005983\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Talanta","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0039914025005983","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

摘要

将Fe掺杂碳点（Fe- cds）加载到金属有机骨架（MOF） MIL-101 （Fe）上，成功合成了一种新型双功能Fe-CDs@MOF复合纳米酶。Fe-CDs的引入增强了MOF的过氧化物酶（POD）样活性，使其具有优异的荧光性质。考虑到Fe-CDs@MOF的双重功能，我们将其与邻苯二胺(OPD)/黄嘌呤氧化酶（XOD）结合，构建了检测黄嘌呤（XAN）的比例荧光探针。XAN首先被XOD氧化生成H2O2，然后Fe-CDs@MOF发挥类似pod的活性催化OPD与H2O2反应生成2,3-二氨基苯丙嗪（DAP）。由于内部过滤作用，DAP又使Fe-CDs@MOF的荧光猝灭。DAP荧光强度与Fe-CDs@MOF荧光强度之比表示XAN的含量。XAN的检测范围为1 ~ 100 μM，检测限为0.36 μM。采用比例荧光探针对鱼类样品中的XAN进行了分析，所得结果与高效液相色谱分析结果吻合较好。该方法还能检测冷冻鱼的新鲜度。本研究为Fe-CDs@MOF复合纳米酶的设计和应用提供了一种新的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Bifunctional Fe-CDs@MOF composite nanozyme based ratiometric fluorescent probe for the detection of xanthine in fish

查看原文本刊更多论文

Bifunctional Fe-CDs@MOF composite nanozyme based ratiometric fluorescent probe for the detection of xanthine in fish

A novel bifunctional Fe-CDs@MOF composite nanozyme was synthesized successfully by loading Fe doped carbon dots (Fe-CDs) onto metal-organic framework (MOF) MIL-101 (Fe). The introduction of Fe-CDs enhanced the peroxidase (POD)-like activity of MOF and endowed it with excellent fluorescence properties. Considering the dual functions of Fe-CDs@MOF, we combined it with o-phenylenediamine (OPD)/xanthine oxidase (XOD) to construct a ratiomeric fluorescence probe for xanthine (XAN) detection. XAN was first oxidized by XOD to produce H₂O₂, then Fe-CDs@MOF exerted its POD-like activity to catalyze the reaction between OPD and H₂O₂ to form 2,3-diaminophenazine (DAP). DAP in turn quenched the fluorescence of Fe-CDs@MOF as a result of internal filtration effect. The ratio of the fluorescence intensity of DAP and the fluorescence intensity of Fe-CDs@MOF indicated the XAN content. The XAN detection range was 1–100 μM with a limit of detection of 0.36 μM. The ratiometric fluorescence probe was utilized to analyze XAN in fish samples, and the results obtained were in good agreement with those assayed by high performance liquid chromatography. The method also had the ability to test the freshness of frozen fish. This study provides a novel approach for the design and application of Fe-CDs@MOF composite nanozyme.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.