RNA去甲基酶ALKBH5在胎儿和成人睾丸中的表达模式

IF 2.7 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-04-04 DOI:10.1016/j.tice.2025.102901

Fang Fang , Ke Ni

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引用次数: 0

摘要

n6 -甲基腺苷（m6A）是真核细胞中常见的rna转录后修饰，参与多种生物过程。ALKBH5是m6A去甲基化酶之一，据报道在小鼠睾丸中发挥重要作用。但ALKBH5在人类睾丸中的功能尚未被发现。本研究旨在分析ALKBH5在胎儿和成人睾丸中的表达和位置。我们发现，人类胎儿睾丸的特点是形成充满前精原细胞和前支持细胞的睾丸索，这与成人睾丸的卷绕精原上皮明显不同。ALKBH5不仅在成人睾丸中广泛表达，在人胎儿睾丸中VASA阳性的前精原细胞、SOX9阳性的前sertoli细胞和CYP11A阳性的前leydig细胞中也有表达。此外，对已发表的rna测序数据（GSE63392）的生物信息学分析显示，ALKBH5在人胎儿生殖细胞中的表达随着孕周的增加而上调。因此，我们的研究结果表明ALKBH5在胎儿人类睾丸发育和功能中的潜在作用。

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Expression pattern of RNA demethylase ALKBH5 in fetal and adult human testis

N⁶-methyladenosine (m⁶A) is a common post-transcriptional modification of RNAs in eukaryotic cells, which is involved in various biological processes. ALKBH5 is one of the m⁶A demethylases and has been reported to play important roles in mouse testis. But the function of ALKBH5 in human testis remained undiscovered. Here we aimed to analyze the expression and location of ALKBH5 in fetal and adult human testis. We found that fetal human testis is characterized by the formation of testis cords filled with pre-spermatogonia and pre-Sertoli cells, which is significantly distinct from the convoluted seminiferous epithelium in adult testis. ALKBH5 is not only widely expressed in adult human testis, but also expressed in VASA positive pre-spermatogonia, SOX9 positive pre-Sertoli cells, and CYP11A positive pre-Leydig cells in fetal human testis. Moreover, bioinformatics analysis of published RNA-sequencing data (GSE63392) revealed the expression of ALKBH5 in human fetal germ cells is upregulated with the increase of gestational weeks. Thus, our results indicate the potential role of ALKBH5 in fetal human testis development and function.

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.