基于比色法和智能手机的抗坏血酸检测双模式传感平台

IF 4.1 3区工程技术 Q2 ENGINEERING, ELECTRICAL & ELECTRONIC

Sensors and Actuators A-physical Pub Date : 2025-04-10 DOI:10.1016/j.sna.2025.116573

Ekta Arjundas Kukreja , Ravi Mani Tripathi , Sang J. Chung

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引用次数: 0

摘要

抗坏血酸（AA）是一种重要的维生素，具有抗氧化特性，用于预防和治疗坏血病和癌症等疾病。因此，精确检测超低浓度的AA是关键挑战之一，对治疗应用也很重要。我们首次评价了ZnO-Pd纳米微球检测AA的纳米酶活性。本研究利用显色底物3,3 ',5,5 ' -四甲基联苯胺（TMB）建立了一种基于纳米酶的AA比色检测方法。ZnO-Pd纳米微球通过氧化显色底物TMB表现出较强的过氧化物酶模拟活性。在过氧化氢（H2O2）存在下，ZnO-Pd纳米微球将无色TMB氧化为蓝色ox-TMB。AA的检测是基于关闭方法，其中AA加入到反应混合物诱导还原反应。这将ox-TMB降低到TMB，导致溶液的蓝色褪色。该方法对多种血清含物进行了检测，对AA具有较高的特异性。在0.05-128 µM的线性范围内研究了该方法的灵敏度，并通过使用UV-Vis光谱测量着色溶液的强度来分析结果。定量限和定量限分别为0.031 µM和0.094 µM。此外，还进行了智能手机分析，以获得反应溶液的颜色强度与AA浓度之间的关系。将该方法用于维生素C片中AA含量的测定，以评价该方法的实用性。

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Colorimetric and smartphone-based dual modality sensing platform for detection of ascorbic acid

Ascorbic acid (AA) is a vital vitamin possessing antioxidant properties which is used to prevent and treat diseases like scurvy and cancer. Therefore, precise detection of ultra-low concentrations of AA is one of the crucial challenges and is also important for therapeutic applications. We have evaluated for the first time the nanozymatic activity of ZnO-Pd nanobolts for the detection of AA. The present study has developed a nanozyme-based colorimetric detection assay for AA using a chromogenic substrate 3,3′,5,5′-Tetramethylbenzidine (TMB). ZnO-Pd nanobolts exhibited strong peroxidase mimetic activity by oxidizing chromogenic substrate TMB. Colorless TMB was oxidised to blue color ox-TMB by the action of ZnO-Pd nanobolts in the presence of hydrogen peroxide (H₂O₂). The detection of AA was based on a turn-off approach where addition of AA to the reaction mixture induced a reduction reaction. This reduces the ox-TMB to TMB causing the blue color of the solution to fade. The assay was tested with multiple serum containing substances and showed high specificity toward AA. The sensitivity of the assay was studied over a linear range of 0.05–128 µM, and the results were analysed by measuring the intensity of the colored solution using UV-Vis spectroscopy. The LOD and LOQ of the assay were found to be 0.031 µM and 0.094 µM respectively. Additionally, smartphone analysis was carried out for attaining a relationship between the color intensity of the reaction solution and concentrations of the AA. The method was used for determination of AA in vitamin C tablets for assessing the practical application of the assay.

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来源期刊

Sensors and Actuators A-physical 工程技术-工程：电子与电气

CiteScore

8.10

自引率

6.50%

发文量

630

审稿时长

49 days

期刊介绍： Sensors and Actuators A: Physical brings together multidisciplinary interests in one journal entirely devoted to disseminating information on all aspects of research and development of solid-state devices for transducing physical signals. Sensors and Actuators A: Physical regularly publishes original papers, letters to the Editors and from time to time invited review articles within the following device areas: • Fundamentals and Physics, such as: classification of effects, physical effects, measurement theory, modelling of sensors, measurement standards, measurement errors, units and constants, time and frequency measurement. Modeling papers should bring new modeling techniques to the field and be supported by experimental results. • Materials and their Processing, such as: piezoelectric materials, polymers, metal oxides, III-V and II-VI semiconductors, thick and thin films, optical glass fibres, amorphous, polycrystalline and monocrystalline silicon. • Optoelectronic sensors, such as: photovoltaic diodes, photoconductors, photodiodes, phototransistors, positron-sensitive photodetectors, optoisolators, photodiode arrays, charge-coupled devices, light-emitting diodes, injection lasers and liquid-crystal displays. • Mechanical sensors, such as: metallic, thin-film and semiconductor strain gauges, diffused silicon pressure sensors, silicon accelerometers, solid-state displacement transducers, piezo junction devices, piezoelectric field-effect transducers (PiFETs), tunnel-diode strain sensors, surface acoustic wave devices, silicon micromechanical switches, solid-state flow meters and electronic flow controllers. Etc...