一种新型生物发光探针用于检测真实食品样品中的甲醛

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-04-08 DOI:10.1016/j.saa.2025.126210

Ziyao Chen , Jie Zhong , Zhangyan Zhu , Chunmei Li , Zhenquan Su , Wenjun Li , Xianggui Chen , Shan Qian

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引用次数: 0

摘要

甲醛（FA）是一种重要的活性羰基物种（RCS）。开发一种快速、高效的方法来定量检测食品和生物样本中的 FA 非常必要。在此，我们用均烯丙基胺笼装萤火虫 D-luciferin (Fluc)，开发了首个生物发光探针 FA-Fluc，该探针可在 4 小时内通过偶氮-科佩重排与 FA 几乎完全反应，产生一种开启的生物发光（BL）。FA-Fluc 的检测限为 0.047 ppm，可以检测不同海产品、农产品和烹饪过程中的 FA。FA-Fluc 对 FA 具有良好的选择性，这表明它不会与其他 RCS 发生反应。此外，在复杂食品基质中检测到的 FA 含量与乙酰丙酮法和高效液相色谱法检测到的含量几乎相同。我们还发现，FA-Fluc 是活细胞和动物体内 FA 实时动态成像的有力工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A new bioluminescent probe for detecting formaldehyde in real food samples

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A new bioluminescent probe for detecting formaldehyde in real food samples

Formaldehyde (FA) is an important type of reactive carbonyl species (RCS). The development of a rapid and efficient method to quantify FA in food and biological samples is necessary. Herein, we have developed the first bioluminescent probe FA-Fluc with homoallyl amine to cage firefly D-luciferin (Fluc), which could react almost completely with FA via aza-Cope rearrangement in 4 h to afford a turn-on Bioluminescence (BL). The detection limit of FA-Fluc was 0.047 ppm; and could detect FA in different seafood, agricultural products, and cooking processes. FA-Fluc has a good selectivity for FA, which shows that it does not react with other RCS. In addition, the FA content detected in complex food substrates is almost the same as that detected by acetylacetone and high-performance liquid chromatography (HPLC) methods. We also found that FA-Fluc was a powerful tool for real-time and dynamic imaging of FA in live cells and animals.

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.