NrCAM和锚蛋白B对胆囊收缩素篮中间神经元周围突触的调控

Erik N. Oldre, Barrett D. Webb, Justin E. Sperringer, Patricia F. Maness
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引用次数: 0

摘要

皮层锥体神经元(PNs)的皮层周围区域整合局部和远程输入并调节放电。该区域接受来自胆囊收缩素(CCK)和小白蛋白(PV)表达的篮状细胞(bc)的gaba能输入,但突触接触是如何建立的尚不清楚。神经元-胶质相关细胞粘附分子(NrCAM)是一种与支架蛋白锚蛋白B结合的亲同质跨膜蛋白。本研究表明NrCAM和锚蛋白B介导小鼠内侧前额叶皮层(mPFC) cck - bc和PNs之间的突触接触。对CCK-BC末端进行泡状谷氨酸转运体-3 (VGLUT3)或泡状GABA转运体(VGAT)的免疫标记显示,nrcam缺失小鼠PN体上CCK-BC突触点显著减少,但PV-BC点或细胞损失未减少。在Nex1Cre-ERT2:Ank2flox/flox:EGFP小鼠中,PNs中的锚蛋白B缺失也减少了VGLUT3+ CCK-BC点。在Synuclein-γ (Sncg)位点表达tdTomato (tdT)的新型CCK-BC报告小鼠显示,NrCAM定位于Sncg + CCK-BC,以及Nex1Cre-ERT2:Ank2+/+:EGFP小鼠的突触后PN体。结果表明NrCAM和锚蛋白B参与了cck - bc与mPFC兴奋性神经元之间连接的建立。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Regulation of perisomatic synapses from cholecystokinin basket interneurons through NrCAM and Ankyrin B

Regulation of perisomatic synapses from cholecystokinin basket interneurons through NrCAM and Ankyrin B
The perisomatic region of cortical pyramidal neurons (PNs) integrates local and long-range inputs and regulates firing. This domain receives GABAergic inputs from cholecystokinin (CCK)- and Parvalbumin (PV)-expressing basket cells (BCs) but how synaptic contacts are established is unclear. Neuron-glial related cell adhesion molecule (NrCAM) is a homophilic transmembrane protein that binds the scaffold protein Ankyrin B. Here we show that NrCAM and Ankyrin B mediate perisomatic synaptic contact between CCK-BCs and PNs in mouse medial prefrontal cortex (mPFC). Immunolabeling of CCK-BC terminals for vesicular glutamate transporter-3 (VGLUT3) or vesicular GABA transporter (VGAT) revealed a significant decrease in CCK-BC synaptic puncta on PN soma in NrCAM-null mice, however no decrease in PV-BC puncta or cell loss. VGLUT3+ CCK-BC puncta were also decreased by Ankyrin B deletion from PNs in Nex1Cre-ERT2:Ank2flox/flox:EGFP mice. A novel CCK-BC reporter mouse expressing tdTomato (tdT) at the Synuclein-γ (Sncg) locus showed NrCAM localized to Sncg + CCK-BCs, and to postsynaptic PN soma in Nex1Cre-ERT2:Ank2+/+:EGFP mice. Results suggest that NrCAM and Ankyrin B contribute to the establishment of connectivity between CCK-BCs and excitatory neurons of the mPFC.
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