秋粘虫对Cry1A的实际抗性。哥伦比亚的105+Cry2Ab2+Cry3Bb1 Bt玉米

IF 2.5 2区农林科学 Q1 AGRONOMY

Crop Protection Pub Date : 2025-04-05 DOI:10.1016/j.cropro.2025.107229

Oscar F. Santos-Amaya , Juan E. Hernandez-Plata , Daniel A. Baron-Ortiz , Claudia Y. Miranda-Montañez , Khalid Haddi

{"title":"秋粘虫对Cry1A的实际抗性。哥伦比亚的105+Cry2Ab2+Cry3Bb1 Bt玉米","authors":"Oscar F. Santos-Amaya , Juan E. Hernandez-Plata , Daniel A. Baron-Ortiz , Claudia Y. Miranda-Montañez , Khalid Haddi","doi":"10.1016/j.cropro.2025.107229","DOIUrl":null,"url":null,"abstract":"<div><div>The use of genetically modified maize hybrids that produce insecticidal proteins from <em>Bacillus thuringiensis</em> (Bt) has been the main strategy for controlling the fall armyworm (FAW), <em>Spodoptera frugiperda</em>, in the Americas. However, the evolution of practical resistance has become a great threat to the sustainable use of Bt technology. Currently, the high dose/refuge and pyramided Bt approaches are the two main IRM strategies used in the Americas to delay the evolution of resistance of FAW to Bt crops. In this study, we examined field-evolved resistance to pyramided Bt maize hybrid in the municipality of Paz de Ariporo, Casanare, Colombia, where plant damage by FAW larvae exceeded the economic threshold in 2023. The offspring reared with leaf tissue of Bt (Cry1A.105 + Cry2Ab2 + Cry3Bb1) and non-Bt maize plants showed neonate-to-adult survival rates as high as 50%. All larvae from the resistant population died within three days on Cry1Ab + Vip3Aa20 maize tissue. Our data confirm that FAW populations can overcome the pyramiding of Cry1 and Cry2 family toxins in Bt maize and that the Vip3Aa20 toxin continues to be effective for populations of this species that have lost susceptibility to the Cry1 and Cry2 toxins. These findings provide novel insights to refine current insect resistance management strategies for pyramided Bt crops.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"194 ","pages":"Article 107229"},"PeriodicalIF":2.5000,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Practical resistance of fall armyworm to Cry1A.105+Cry2Ab2+Cry3Bb1 Bt maize in Colombia\",\"authors\":\"Oscar F. Santos-Amaya , Juan E. Hernandez-Plata , Daniel A. Baron-Ortiz , Claudia Y. Miranda-Montañez , Khalid Haddi\",\"doi\":\"10.1016/j.cropro.2025.107229\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The use of genetically modified maize hybrids that produce insecticidal proteins from <em>Bacillus thuringiensis</em> (Bt) has been the main strategy for controlling the fall armyworm (FAW), <em>Spodoptera frugiperda</em>, in the Americas. However, the evolution of practical resistance has become a great threat to the sustainable use of Bt technology. Currently, the high dose/refuge and pyramided Bt approaches are the two main IRM strategies used in the Americas to delay the evolution of resistance of FAW to Bt crops. In this study, we examined field-evolved resistance to pyramided Bt maize hybrid in the municipality of Paz de Ariporo, Casanare, Colombia, where plant damage by FAW larvae exceeded the economic threshold in 2023. The offspring reared with leaf tissue of Bt (Cry1A.105 + Cry2Ab2 + Cry3Bb1) and non-Bt maize plants showed neonate-to-adult survival rates as high as 50%. All larvae from the resistant population died within three days on Cry1Ab + Vip3Aa20 maize tissue. Our data confirm that FAW populations can overcome the pyramiding of Cry1 and Cry2 family toxins in Bt maize and that the Vip3Aa20 toxin continues to be effective for populations of this species that have lost susceptibility to the Cry1 and Cry2 toxins. These findings provide novel insights to refine current insect resistance management strategies for pyramided Bt crops.</div></div>\",\"PeriodicalId\":10785,\"journal\":{\"name\":\"Crop Protection\",\"volume\":\"194 \",\"pages\":\"Article 107229\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-04-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Crop Protection\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0261219425001218\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crop Protection","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0261219425001218","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

摘要

在美洲，使用从苏云金芽孢杆菌（Bt）中产生杀虫蛋白的转基因玉米杂交品种一直是控制秋粘虫（FAW）的主要策略。然而，实际抗性的演变已经成为Bt技术可持续利用的巨大威胁。目前，在美洲，高剂量/避难和锥形Bt方法是延缓FAW对Bt作物产生抗性的两种主要IRM策略。在这项研究中，我们研究了哥伦比亚Casanare市Paz de Ariporo市对Bt玉米金字塔杂交品种的田间进化抗性，该地区FAW幼虫对植物的危害在2023年超过了经济阈值。用Bt （Cry1A）叶片组织饲养后代。105 + Cry2Ab2 + Cry3Bb1)和非bt玉米植株的成虫存活率高达50%。抗性群体的所有幼虫在Cry1Ab + Vip3Aa20玉米组织上3天内全部死亡。我们的数据证实，FAW群体可以克服Cry1和Cry2家族毒素在Bt玉米中的金字塔化，并且Vip3Aa20毒素对该物种对Cry1和Cry2毒素失去敏感性的群体仍然有效。这些发现为完善目前转基因Bt作物的抗虫管理策略提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Practical resistance of fall armyworm to Cry1A.105+Cry2Ab2+Cry3Bb1 Bt maize in Colombia

The use of genetically modified maize hybrids that produce insecticidal proteins from Bacillus thuringiensis (Bt) has been the main strategy for controlling the fall armyworm (FAW), Spodoptera frugiperda, in the Americas. However, the evolution of practical resistance has become a great threat to the sustainable use of Bt technology. Currently, the high dose/refuge and pyramided Bt approaches are the two main IRM strategies used in the Americas to delay the evolution of resistance of FAW to Bt crops. In this study, we examined field-evolved resistance to pyramided Bt maize hybrid in the municipality of Paz de Ariporo, Casanare, Colombia, where plant damage by FAW larvae exceeded the economic threshold in 2023. The offspring reared with leaf tissue of Bt (Cry1A.105 + Cry2Ab2 + Cry3Bb1) and non-Bt maize plants showed neonate-to-adult survival rates as high as 50%. All larvae from the resistant population died within three days on Cry1Ab + Vip3Aa20 maize tissue. Our data confirm that FAW populations can overcome the pyramiding of Cry1 and Cry2 family toxins in Bt maize and that the Vip3Aa20 toxin continues to be effective for populations of this species that have lost susceptibility to the Cry1 and Cry2 toxins. These findings provide novel insights to refine current insect resistance management strategies for pyramided Bt crops.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.