{"title":"光学空间微分非相干显微镜","authors":"Yongqi Zhao, Shuoqing Liu, Qiang Yang, Yichang Shou, Shizhen Chen, Shuangchun Wen, Hailu Luo","doi":"10.1002/lpor.202500100","DOIUrl":null,"url":null,"abstract":"<p>Optical spatial differential microscopy is an effective tool for non-toxicity and label-free imaging of biological samples. Existing methods mainly focus on coherent light illumination, which inevitably influences imaging quality by laser scattering effects. In this paper, a compact and broadband optical spatial differential microscopy under incoherent illumination is proposed by only inserting a pair of polarizers into a conventional microscopy. The 2D differentiation operation is performed with the polarizers extracting the cross-polarization term from transmitted light field. Moreover, the effects of light source coherence and wavelength on spatial differentiation are discussed in detail. The results show that this scheme can realize high-quality spatial differentiation imaging for the biological cells, transparent plastic scratches, and real-time monitoring of bubbles. It is experimentally demonstrated that the system can still perform stably when the incident angle exists a large misalignment with a single short-focus lens imaging. This method may further reduce the size of the differential system and drive the advancement of optical microscopy with incoherent light.</p>","PeriodicalId":204,"journal":{"name":"Laser & Photonics Reviews","volume":"19 15","pages":""},"PeriodicalIF":10.0000,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Optical Spatial Differential Incoherent Microscopy\",\"authors\":\"Yongqi Zhao, Shuoqing Liu, Qiang Yang, Yichang Shou, Shizhen Chen, Shuangchun Wen, Hailu Luo\",\"doi\":\"10.1002/lpor.202500100\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Optical spatial differential microscopy is an effective tool for non-toxicity and label-free imaging of biological samples. Existing methods mainly focus on coherent light illumination, which inevitably influences imaging quality by laser scattering effects. In this paper, a compact and broadband optical spatial differential microscopy under incoherent illumination is proposed by only inserting a pair of polarizers into a conventional microscopy. The 2D differentiation operation is performed with the polarizers extracting the cross-polarization term from transmitted light field. Moreover, the effects of light source coherence and wavelength on spatial differentiation are discussed in detail. The results show that this scheme can realize high-quality spatial differentiation imaging for the biological cells, transparent plastic scratches, and real-time monitoring of bubbles. It is experimentally demonstrated that the system can still perform stably when the incident angle exists a large misalignment with a single short-focus lens imaging. This method may further reduce the size of the differential system and drive the advancement of optical microscopy with incoherent light.</p>\",\"PeriodicalId\":204,\"journal\":{\"name\":\"Laser & Photonics Reviews\",\"volume\":\"19 15\",\"pages\":\"\"},\"PeriodicalIF\":10.0000,\"publicationDate\":\"2025-04-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Laser & Photonics Reviews\",\"FirstCategoryId\":\"101\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/lpor.202500100\",\"RegionNum\":1,\"RegionCategory\":\"物理与天体物理\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"OPTICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Laser & Photonics Reviews","FirstCategoryId":"101","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/lpor.202500100","RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"OPTICS","Score":null,"Total":0}
Optical spatial differential microscopy is an effective tool for non-toxicity and label-free imaging of biological samples. Existing methods mainly focus on coherent light illumination, which inevitably influences imaging quality by laser scattering effects. In this paper, a compact and broadband optical spatial differential microscopy under incoherent illumination is proposed by only inserting a pair of polarizers into a conventional microscopy. The 2D differentiation operation is performed with the polarizers extracting the cross-polarization term from transmitted light field. Moreover, the effects of light source coherence and wavelength on spatial differentiation are discussed in detail. The results show that this scheme can realize high-quality spatial differentiation imaging for the biological cells, transparent plastic scratches, and real-time monitoring of bubbles. It is experimentally demonstrated that the system can still perform stably when the incident angle exists a large misalignment with a single short-focus lens imaging. This method may further reduce the size of the differential system and drive the advancement of optical microscopy with incoherent light.
期刊介绍:
Laser & Photonics Reviews is a reputable journal that publishes high-quality Reviews, original Research Articles, and Perspectives in the field of photonics and optics. It covers both theoretical and experimental aspects, including recent groundbreaking research, specific advancements, and innovative applications.
As evidence of its impact and recognition, Laser & Photonics Reviews boasts a remarkable 2022 Impact Factor of 11.0, according to the Journal Citation Reports from Clarivate Analytics (2023). Moreover, it holds impressive rankings in the InCites Journal Citation Reports: in 2021, it was ranked 6th out of 101 in the field of Optics, 15th out of 161 in Applied Physics, and 12th out of 69 in Condensed Matter Physics.
The journal uses the ISSN numbers 1863-8880 for print and 1863-8899 for online publications.