光学空间微分非相干显微镜

IF 10 1区 物理与天体物理 Q1 OPTICS
Yongqi Zhao, Shuoqing Liu, Qiang Yang, Yichang Shou, Shizhen Chen, Shuangchun Wen, Hailu Luo
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引用次数: 0

摘要

光学空间微分显微镜是对生物样本进行无毒、无标记成像的有效工具。现有方法主要集中在相干光照明上,激光散射效应不可避免地会影响成像质量。本文提出了一种在非相干光照明下的紧凑型宽带光学空间差分显微镜,只需在传统显微镜中插入一对偏振片即可。利用偏振镜从透射光场中提取交叉偏振项,进行二维微分操作。此外,还详细讨论了光源相干性和波长对空间分化的影响。结果表明,该方案可以实现对生物细胞、透明塑料划痕的高质量空间分化成像,以及对气泡的实时监测。实验证明,当入射角存在较大偏差时,该系统仍能稳定地进行单短焦透镜成像。这种方法可进一步缩小差分系统的体积,推动非相干光光学显微镜的发展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Optical Spatial Differential Incoherent Microscopy

Optical Spatial Differential Incoherent Microscopy

Optical Spatial Differential Incoherent Microscopy

Optical spatial differential microscopy is an effective tool for non-toxicity and label-free imaging of biological samples. Existing methods mainly focus on coherent light illumination, which inevitably influences imaging quality by laser scattering effects. In this paper, a compact and broadband optical spatial differential microscopy under incoherent illumination is proposed by only inserting a pair of polarizers into a conventional microscopy. The 2D differentiation operation is performed with the polarizers extracting the cross-polarization term from transmitted light field. Moreover, the effects of light source coherence and wavelength on spatial differentiation are discussed in detail. The results show that this scheme can realize high-quality spatial differentiation imaging for the biological cells, transparent plastic scratches, and real-time monitoring of bubbles. It is experimentally demonstrated that the system can still perform stably when the incident angle exists a large misalignment with a single short-focus lens imaging. This method may further reduce the size of the differential system and drive the advancement of optical microscopy with incoherent light.

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来源期刊
CiteScore
14.20
自引率
5.50%
发文量
314
审稿时长
2 months
期刊介绍: Laser & Photonics Reviews is a reputable journal that publishes high-quality Reviews, original Research Articles, and Perspectives in the field of photonics and optics. It covers both theoretical and experimental aspects, including recent groundbreaking research, specific advancements, and innovative applications. As evidence of its impact and recognition, Laser & Photonics Reviews boasts a remarkable 2022 Impact Factor of 11.0, according to the Journal Citation Reports from Clarivate Analytics (2023). Moreover, it holds impressive rankings in the InCites Journal Citation Reports: in 2021, it was ranked 6th out of 101 in the field of Optics, 15th out of 161 in Applied Physics, and 12th out of 69 in Condensed Matter Physics. The journal uses the ISSN numbers 1863-8880 for print and 1863-8899 for online publications.
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