稀土纳米颗粒生物转化的遗传调控影响生物结果

IF 15.7 1区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Mingming Tian, Di Wu, Xiao Gou, Ruibin Li, Xiaowei Zhang
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引用次数: 0

摘要

纳米颗粒的生物转化在决定其生物命运和反应中起着至关重要的作用。虽然一些工程策略(例如,表面功能化和形状控制)已经被用来调节纳米颗粒的命运,纳米颗粒生物转化的遗传控制仍然是一个未探索的途径。在此,我们利用基于crispr的基因组级敲除方法来鉴定参与稀土氧化物(REO)纳米颗粒生物转化的基因。我们发现REOs在溶酶体中的生物转化可以通过SMPD1进行遗传控制。具体来说,SMPD1的抑制抑制了La2O3向海胆形状结构的转化,从而防止溶酶体损伤、促炎细胞因子释放、焦亡和re诱导的尘肺病。总的来说,我们的研究为如何控制纳米材料的生物命运提供了见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Genetic modulation of rare earth nanoparticle biotransformation shapes biological outcomes

Genetic modulation of rare earth nanoparticle biotransformation shapes biological outcomes

The biotransformation of nanoparticles plays a crucial role in determining their biological fate and responses. Although a few engineering strategies (e.g., surface functionalization and shape control) have been employed to regulate the fate of nanoparticles, the genetic control of nanoparticle biotransformation remains an unexplored avenue. Herein, we utilized a CRISPR-based genome-scale knockout approach to identify genes involved in the biotransformation of rare earth oxide (REO) nanoparticles. We found that the biotransformation of REOs in lysosomes could be genetically controlled via SMPD1. Specifically, suppression of SMPD1 inhibited the transformation of La2O3 into sea urchin-shaped structures, thereby protecting against lysosomal damage, proinflammatory cytokine release, pyroptosis and RE-induced pneumoconiosis. Overall, our study provides insight into how to control the biological fate of nanomaterials.

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来源期刊
Nature Communications
Nature Communications Biological Science Disciplines-
CiteScore
24.90
自引率
2.40%
发文量
6928
审稿时长
3.7 months
期刊介绍: Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.
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