基于微靶向捕获测序的引起普通感冒症状的冠状病毒的遗传特征

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2025-04-11 DOI:10.1007/s00705-025-06292-8

Qiao Qiao, Xiaojuan Zhu, Zixinrong Meng, Hongbin Zhang, Tao Wu, Kangchen Zhao, Yiyue Ge, Lunbiao Cui

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引用次数: 0

摘要

建立了一种针对引起普通感冒症状的四种冠状病毒的微靶向捕获测序方法，并利用14份咽拭子样本对其敏感性和临床性能进行了评估。该技术对循环阈值（Ct）为32或更低的样本实现了100%的基因组覆盖率。通过系统发育分析确定了临床分离株的基因型，并在刺突蛋白的受体结合域（RBD）中发现了独特的突变。在一些分离株中发现了额外的n -糖基化位点。该方法为普通感冒冠状病毒的遗传监测提供了灵敏、快速的工具，有助于预防和控制。

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Genetic characterization of coronaviruses causing common cold symptoms based on micro-targeted capture sequencing

A novel micro-targeted capture sequencing method was developed for four coronaviruses that cause common cold symptoms, and its sensitivity and clinical performance were assessed using 14 pharyngeal swab samples. This technique achieved 100% genomic coverage for samples with a cycle threshold (Ct) value of 32 or lower. The genotypes of the clinical isolates were determined by phylogenetic analysis, and unique mutations were identified in the receptor-binding domain (RBD) of the spike protein. Additional N-glycosylation sites were found in some of the isolates. This method offers a sensitive and rapid tool for genetic monitoring of common-cold coronaviruses and aids in their prevention and control.

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.