检测胎儿红细胞的电免疫传感器对胎儿出血的潜在诊断

IF 4.8 2区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Suelen Assunpção Nishio , Andrei Moroz , Marjorie de Assis Golim , Aline Marcia Marques Braz , Anna Laura Yuri Yokomichi , Valber A. Pedrosa , Ivana Cesarino , Osvaldo N. Oliveira Jr. , Sidney J.L. Ribeiro , Elenice Deffune , Marli Leite de Moraes
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引用次数: 0

摘要

胎儿出血的特点是胎儿血液进入母体循环,对母亲和胎儿都有重大风险。由于可以通过定量检测母体循环中的胎儿红细胞来诊断,我们开发了一种免疫传感器来检测由逐层丝素蛋白膜和商用CD71单克隆抗体制成的胎儿细胞。检测通过电阻抗谱实现,可以区分来自患者和志愿者的人类脐带血(UCB)细胞和成人外周血(APB)细胞。1 ~ 10 Hz时,阳性样品(UCB)的平均电容值为0.34 nF,阴性样品(APB)的平均电容值为0.05 nF。与流式细胞术检测胎儿红细胞完全一致。电反应的变化与UCB细胞在免疫传感器上的吸附有关,主要是由于酰胺I基团的构象变化。偏振调制红外反射吸收光谱(PM-IRRAS)显示,这反映了免疫亲和相互作用。光学显微镜图像证实细胞吸附,这表明可能的护理点策略诊断FMH与图像分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Electrical immunosensor for detecting fetal red blood cells with potential diagnosis of fetomaternal hemorrhage

Electrical immunosensor for detecting fetal red blood cells with potential diagnosis of fetomaternal hemorrhage
Fetomaternal hemorrhage, characterized by the passage of fetal blood into maternal circulation, poses significant risks to both mother and fetus. As it may be diagnosed by quantifying fetal red blood cells in maternal circulation, we developed an immunosensor to detect fetal cells made with a layer-by-layer silk fibroin film and a commercial CD71 monoclonal antibody. Detection was achieved through electrical impedance spectroscopy, allowing discrimination between human umbilical cord blood (UCB) cells and adult peripheral blood (APB) cells from patients and volunteers. Positive samples (UCB) exhibited an average capacitance of 0.34 nF at 1 to 10 Hz, while negative samples (APB) had 0.05 nF. Full agreement was obtained with the detection of fetal red blood cells using flow cytometry. The change in the electrical response was associated with the adsorption of UCB cells on the immunosensor, primarily due to conformational changes in the amide I groups. This reflects the immunoaffinity interactions, as revealed with polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). Cell adsorption was confirmed with optical microscopy images, which indicate possible point-of-care strategies to diagnose FMH with image analysis.
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来源期刊
Bioelectrochemistry
Bioelectrochemistry 生物-电化学
CiteScore
9.10
自引率
6.00%
发文量
238
审稿时长
38 days
期刊介绍: An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.
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