利用仙台病毒重编程从健康男性人角质形成细胞中生成iPSC系INDBi001-A并进行鉴定

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-04-04 DOI:10.1016/j.scr.2025.103709

Denise Sperlich , Katharina Becker , Ulrike Mau-Holzmann , Stefan Liebau , Kevin Achberger

{"title":"利用仙台病毒重编程从健康男性人角质形成细胞中生成iPSC系INDBi001-A并进行鉴定","authors":"Denise Sperlich , Katharina Becker , Ulrike Mau-Holzmann , Stefan Liebau , Kevin Achberger","doi":"10.1016/j.scr.2025.103709","DOIUrl":null,"url":null,"abstract":"<div><div>We generated a fully characterized iPSC line derived from human keratinocytes using Sendai virus-based reprogramming. This integration-free method preserves genomic integrity, enabling the generation of a pluripotent line with robust self-renewal and differentiation capabilities. Comprehensive characterization confirmed the iPSC line’s pluripotency markers, trilineage differentiation potential, and karyotypic normality. This resource provides a valuable tool for disease modeling, drug discovery, and regenerative medicine applications.</div></div>","PeriodicalId":21843,"journal":{"name":"Stem cell research","volume":"85 ","pages":"Article 103709"},"PeriodicalIF":0.8000,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Generation and characterization of the iPSC line INDBi001-A from human keratinocytes of a healthy male using Sendai virus reprogramming\",\"authors\":\"Denise Sperlich , Katharina Becker , Ulrike Mau-Holzmann , Stefan Liebau , Kevin Achberger\",\"doi\":\"10.1016/j.scr.2025.103709\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>We generated a fully characterized iPSC line derived from human keratinocytes using Sendai virus-based reprogramming. This integration-free method preserves genomic integrity, enabling the generation of a pluripotent line with robust self-renewal and differentiation capabilities. Comprehensive characterization confirmed the iPSC line’s pluripotency markers, trilineage differentiation potential, and karyotypic normality. This resource provides a valuable tool for disease modeling, drug discovery, and regenerative medicine applications.</div></div>\",\"PeriodicalId\":21843,\"journal\":{\"name\":\"Stem cell research\",\"volume\":\"85 \",\"pages\":\"Article 103709\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2025-04-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Stem cell research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1873506125000595\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Stem cell research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1873506125000595","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

我们利用基于仙台病毒的重编程，从人角质形成细胞中获得了一个完全表征的iPSC系。这种无整合的方法保持了基因组的完整性，使多能系的产生具有强大的自我更新和分化能力。综合鉴定证实了iPSC系的多能性标记、三龄分化潜力和核型正常。该资源为疾病建模、药物发现和再生医学应用提供了有价值的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Generation and characterization of the iPSC line INDBi001-A from human keratinocytes of a healthy male using Sendai virus reprogramming

We generated a fully characterized iPSC line derived from human keratinocytes using Sendai virus-based reprogramming. This integration-free method preserves genomic integrity, enabling the generation of a pluripotent line with robust self-renewal and differentiation capabilities. Comprehensive characterization confirmed the iPSC line’s pluripotency markers, trilineage differentiation potential, and karyotypic normality. This resource provides a valuable tool for disease modeling, drug discovery, and regenerative medicine applications.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.