基于葡萄糖浓度分析的唾液成分均匀性和稳定性

IF 3.2 3区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Clinica Chimica Acta Pub Date : 2025-04-07 DOI:10.1016/j.cca.2025.120283

Kuncheng Chen , Song Wang , Pian Zhong , Yeping Peng , Junzhe Lu , Lanlan Liu , Jinmei He , Weiqiang Liu

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引用次数: 0

摘要

唾液具有非侵入性和自我收集的特点，有望成为葡萄糖监测的生物标志物。但其黏性可能导致葡萄糖分布不均匀，反映唾液的均匀性，饮食等个体血糖控制因素可能影响唾液葡萄糖浓度，反映唾液的稳定性。为了解决这些问题，本研究旨在评估唾液葡萄糖浓度的均匀性和稳定性。利用流变仪、扫描电镜和能量色散x射线能谱（SEM-EDS）技术分析了唾液的宏观流变特性（剪切粘度、弹性模量和粘性模量）和微观形态特征。在室温静态条件下、一个月的空腹唾液和餐后状态下评估唾液葡萄糖的均匀性和稳定性。结果表明，随着剪切速率从0.1到150 s−1的增加，唾液的剪切粘度从0.1 Pa∙s降低到0.001 Pa∙s。这种剪切变薄的特性与通过微观形态分析观察到的多孔糖蛋白网络有关，可能会影响分析物的分布。尽管具有固有的黏性，唾液葡萄糖浓度表现出高度的均匀性：89%的空腹样本和92%的餐后样本在分成三份时显示出小于10%的变化。关于稳定性，健康个体的唾液葡萄糖水平在各种情况下始终比糖尿病患者的唾液葡萄糖水平更稳定，包括空腹唾液的静态室温储存、禁食唾液超过一个月和餐后唾液。例如，糖尿病患者一个月的空腹血糖水平（7.38±6.3 mg/dL）高于健康个体（1.674±0.72 mg/dL），但不太稳定。本研究证实，空腹唾液葡萄糖既均匀又足够稳定，支持其作为血糖监测的可行生物标志物的潜力。

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Uniformity and stability of saliva composition based on glucose concentration analysis

Saliva holds promise as a biomarker for glucose monitoring due to its non-invasive and self-collection characteristics. However, its viscosity may lead to uneven glucose distribution, reflecting the uniformity of saliva, and individual glycemic control factors such as diet may affect salivary glucose concentration, reflecting the stability of saliva. To address these concerns, this study aims to evaluate the uniformity and stability of salivary glucose concentrations.

Macro-rheological properties (shear viscosity, elastic modulus, and viscous modulus) and micro-morphological characteristics of saliva were analyzed using a rheometer and Scanning Electron Microscopy and Energy-Dispersive X-ray Spectroscopy (SEM-EDS) techniques. Salivary glucose uniformity and stability were assessed under room-temperature static conditions, fasting saliva over a one-month period, and in postprandial states.

Results showed that saliva exhibits shear-thinning behavior, with shear viscosity decreasing from 0.1 Pa∙s to 0.001 Pa∙s as shear rate increases from 0.1 to 150 s⁻¹. This shear-thinning property, linked to the porous glycoprotein network observed through micro-morphological analysis, may influence analyte distribution. Despite the inherent viscosity, salivary glucose concentrations demonstrated high uniformity: 89 % of fasting samples and 92 % of postprandial samples showed less than 10 % variation when divided into three portions.

Regarding stability, salivary glucose levels in healthy individuals remained consistently more stable than those in diabetic patients across various conditions, including static room-temperature storage of fasting saliva, fasting saliva over one month, and postprandial saliva. For example, fasting glucose levels over one month in diabetic patients (7.38 ± 6.3 mg/dL) were higher and less stable than those observed in healthy individuals (1.674 ± 0.72 mg/dL).

This study confirms that fasting salivary glucose is both homogeneous and sufficiently stable, supporting its potential as a viable biomarker for blood glucose monitoring.

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来源期刊

Clinica Chimica Acta 医学-医学实验技术

CiteScore

10.10

自引率

2.00%

发文量

1268

审稿时长

23 days

期刊介绍： The Official Journal of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) Clinica Chimica Acta is a high-quality journal which publishes original Research Communications in the field of clinical chemistry and laboratory medicine, defined as the diagnostic application of chemistry, biochemistry, immunochemistry, biochemical aspects of hematology, toxicology, and molecular biology to the study of human disease in body fluids and cells. The objective of the journal is to publish novel information leading to a better understanding of biological mechanisms of human diseases, their prevention, diagnosis, and patient management. Reports of an applied clinical character are also welcome. Papers concerned with normal metabolic processes or with constituents of normal cells or body fluids, such as reports of experimental or clinical studies in animals, are only considered when they are clearly and directly relevant to human disease. Evaluation of commercial products have a low priority for publication, unless they are novel or represent a technological breakthrough. Studies dealing with effects of drugs and natural products and studies dealing with the redox status in various diseases are not within the journal''s scope. Development and evaluation of novel analytical methodologies where applicable to diagnostic clinical chemistry and laboratory medicine, including point-of-care testing, and topics on laboratory management and informatics will also be considered. Studies focused on emerging diagnostic technologies and (big) data analysis procedures including digitalization, mobile Health, and artificial Intelligence applied to Laboratory Medicine are also of interest.