2023年欧洲肠道病毒D68的持续传播以及与VP1蛋白不同氨基酸替换相关的肠道病毒D68 b3谱系的持续进化

IF 4 3区医学 Q2 VIROLOGY

Journal of Clinical Virology Pub Date : 2025-04-05 DOI:10.1016/j.jcv.2025.105785

Aurora Hirvonen , Caroline Klint Johannesen , Peter Simmonds , Thea K Fischer , Heli Harvala , Kimberley S.M. Benschop , on behalf of ENPEN study collaborators

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引用次数: 0

摘要

背景肠病毒D68 （EV-D68）引起从轻度到严重的呼吸系统疾病，在极少数情况下引起麻痹综合征，称为急性弛缓性脊髓炎（AFM）。自2014年全球暴发EV-D68以来，该病毒主要以两年一次的流行周期传播，在偶数年发现高峰。然而，在2019冠状病毒病大流行之后，EV-D68的季节性特征是每年大幅上升。在这里，我们描述了EV-D68在2023年欧洲的循环，并跟踪其基因进化。研究设计数据来自欧洲非脊髓灰质炎网络（ENPEN）的成员。这包括2023年检测到的EV样本总数、EV阳性样本、EV- d68阳性样本和病例以及其他EV阳性样本的月度数据。记录了样本类型和监测系统的信息。利用VP1基因序列数据进行系统发育和氨基酸序列分析。结果203622份诊断样本中检出13585份（6.7%），其中402份（3.0%）检出EV-D68，共386例。EV-D68感染在2023年10月达到高峰(136/386；35.2%)。临床监测捕获EV- d68病例中有267/386例（69.2%），其中绝大多数（202/204例有标本类型信息的阳性标本）为呼吸道标本。对99个VP1序列进行的系统发育分析显示，在欧洲，具有先前未描述的残基变化D554E的独特b3衍生谱系。该研究记录了2023年EV-D68在欧洲的持续循环，b3衍生谱系的进化以及之前未描述的氨基酸取代在欧洲的出现。这强调需要持续监测EV-D68并统一EV-D68检测做法，以提高各国数据的可比性。

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Sustained circulation of enterovirus D68 in Europe in 2023 and the continued evolution of enterovirus D68 B3-lineages associated with distinct amino acid substitutions in VP1 protein

Background

Enterovirus D68 (EV-D68) causes respiratory disease ranging from mild to severe and in rare cases a paralytic syndrome, called acute flaccid myelitis (AFM). Since the global EV-D68 outbreak in 2014, the virus has mainly circulated in biennial epidemic cycles with peaks detected during even years. However, following the COVID-19 pandemic, the seasonal pattern of EV-D68 has been characterized by large yearly upsurges. Here, we describe the circulation of EV-D68 in Europe in 2023 and track its genetic evolution.

Study design

Data was compiled from members of the European Non-Polio Network (ENPEN). This included monthly data on the total number of EV samples tested, EV positive samples, EV-D68 positive samples and cases, and other EV positive samples detected in 2023. Information on sample types and surveillance system was recorded. Sequence data from the VP1 gene was used for phylogenetic and amino acid sequence analysis.

Results

EV was detected in 13585 out of 203622 diagnostic samples tested (6.7%), of which 402 (3.0%) were determined as EV-D68, representing 386 cases. EV-D68 infections peaked in October 2023 (136/386; 35.2%). 267/386 (69.2%) of EV-D68 cases were captured through clinical EV surveillance, almost all of which (202/204 of positive samples with sample type information) were detected in respiratory specimens. Phylogenetic analysis performed on 99 VP1 sequences revealed a distinct B3-derived lineage with a previously undescribed residue change, D554E, in Europe.

Conclusions

The study documents sustained circulation of EV-D68 in Europe in 2023, the evolution of B3-derived lineages, and appearance of previously undescribed amino acid substitutions in Europe. This stresses the need for continuous EV-D68 surveillance and harmonization of EV-D68 detection practices towards better data comparability across countries.

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来源期刊

Journal of Clinical Virology 医学-病毒学

CiteScore

22.70

自引率

1.10%

发文量

149

审稿时长

24 days

期刊介绍： The Journal of Clinical Virology, an esteemed international publication, serves as the official journal for both the Pan American Society for Clinical Virology and The European Society for Clinical Virology. Dedicated to advancing the understanding of human virology in clinical settings, the Journal of Clinical Virology focuses on disseminating research papers and reviews pertaining to the clinical aspects of virology. Its scope encompasses articles discussing diagnostic methodologies and virus-induced clinical conditions, with an emphasis on practicality and relevance to clinical practice. The journal publishes on topics that include: • new diagnostic technologies • nucleic acid amplification and serologic testing • targeted and metagenomic next-generation sequencing • emerging pandemic viral threats • respiratory viruses • transplant viruses • chronic viral infections • cancer-associated viruses • gastrointestinal viruses • central nervous system viruses • one health (excludes animal health)