体外循环对动物血浆主要成分结构的影响——ATR-IR和2D-COS研究。第二部分：抗血栓药物的存在

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-04-09 DOI:10.1016/j.saa.2025.126185

S. Olsztyńska-Janus , M.A. Czarnecki

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引用次数: 0

摘要

体外循环（ECC）的应用需要使用抗血栓药物来维持血液流动性和预防血栓并发症。这一过程的分子机制尚未完全阐明；因此，我们研究了抗血栓药物（阿司匹林和氯吡格雷）对ECC期间主要血浆成分结构变化的影响。采用ATR-IR（衰减全反射红外）光谱监测光谱变化，并采用移动窗口法和二维相关光谱（2D-COS）分析光谱变化。对于接受抗凝治疗的样品，与对照样品相比，我们观察到更高程度的光谱变化。最显著的变化是来自水和酰胺部分的条带。结果表明，ATR-IR光谱与先进的数值分析相结合，具有较高的灵敏度和可靠性，可用于监测ECC期间血浆结构和成分的变化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Effect of extracorporeal circulation on structure of main components of animal plasma – ATR-IR and 2D-COS studies. Part II: Presence of antithrombotic drugs

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Effect of extracorporeal circulation on structure of main components of animal plasma – ATR-IR and 2D-COS studies. Part II: Presence of antithrombotic drugs

An application of extracorporeal circulation (ECC) requires using antithrombotic drugs to maintain blood fluidity and prevent thrombotic complications. The molecular mechanism of this process is not fully elucidated; therefore, we undertook an examination of the effect of antithrombotic drugs (aspirin and clopidogrel) on changes in the structure of the main plasma components during ECC. The spectral variations were monitored using ATR-IR (Attenuated Total Reflection Infrared) spectroscopy and analyzed by moving-window approach and two-dimensional correlation spectroscopy (2D-COS). For samples subjected to anticoagulant therapy, we observed a much higher extent of spectral changes, as compared with the control samples. The most significant changes were observed for the bands from water and amide moieties. The obtained results demonstrate high sensitivity and reliability of ATR-IR spectroscopy coupled with advanced numerical analysis for monitoring variations in the structure and composition of blood plasma during ECC.

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.