Huaiyuan Zhang, Yiting Hu, Xingwei Pu, Shizheng Zhang, Yi He, Kun Chen, Ziji Liu
{"title":"基于led的多色扩展分辨率透射荧光显微镜。","authors":"Huaiyuan Zhang, Yiting Hu, Xingwei Pu, Shizheng Zhang, Yi He, Kun Chen, Ziji Liu","doi":"10.1117/1.JBO.30.4.046501","DOIUrl":null,"url":null,"abstract":"<p><strong>Significance: </strong>The multiplexing capabilities of fluorescence imaging are enhanced by its exceptional molecular specificity with diverse fluorescent probes, making it a powerful tool for studying complex biological structures, organization, and functions. Recent advances in super-resolution fluorescence microscopy have further revolutionized our ability to explore biology and related fields. However, current multicolor super-resolution fluorescence imaging systems often come with high costs and bulky designs.</p><p><strong>Aim: </strong>We present a multicolor extended resolution fluorescence imaging system that uses light-emitting diode to simplify the optical path, make the design more compact, and reduce system costs.</p><p><strong>Approach: </strong>This multicolor extended resolution fluorescence imaging system is based on structured illumination, utilizing a simple diffraction unit positioned between the light source and the sample in a wide-field microscope. Notably, this design could be easily integrated into standard widefield microscopes as a convenient add-on unit, enabling extended resolution imaging.</p><p><strong>Results: </strong>Our system demonstrates concurrent extended resolved imaging of three-color microsphere beads and successfully showcases multicolor extended resolution fluorescence imaging of biological tissue samples, revealing intricate structural details.</p><p><strong>Conclusions: </strong>This system provides a structurally simple, cost-effective alternative to traditional microscopes, offering flexible multicolor extended resolution fluorescence imaging and potential applications in multimodal imaging.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 4","pages":"046501"},"PeriodicalIF":2.9000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11977515/pdf/","citationCount":"0","resultStr":"{\"title\":\"LED-based multicolor extended resolution transmission fluorescence microscopy.\",\"authors\":\"Huaiyuan Zhang, Yiting Hu, Xingwei Pu, Shizheng Zhang, Yi He, Kun Chen, Ziji Liu\",\"doi\":\"10.1117/1.JBO.30.4.046501\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Significance: </strong>The multiplexing capabilities of fluorescence imaging are enhanced by its exceptional molecular specificity with diverse fluorescent probes, making it a powerful tool for studying complex biological structures, organization, and functions. Recent advances in super-resolution fluorescence microscopy have further revolutionized our ability to explore biology and related fields. However, current multicolor super-resolution fluorescence imaging systems often come with high costs and bulky designs.</p><p><strong>Aim: </strong>We present a multicolor extended resolution fluorescence imaging system that uses light-emitting diode to simplify the optical path, make the design more compact, and reduce system costs.</p><p><strong>Approach: </strong>This multicolor extended resolution fluorescence imaging system is based on structured illumination, utilizing a simple diffraction unit positioned between the light source and the sample in a wide-field microscope. Notably, this design could be easily integrated into standard widefield microscopes as a convenient add-on unit, enabling extended resolution imaging.</p><p><strong>Results: </strong>Our system demonstrates concurrent extended resolved imaging of three-color microsphere beads and successfully showcases multicolor extended resolution fluorescence imaging of biological tissue samples, revealing intricate structural details.</p><p><strong>Conclusions: </strong>This system provides a structurally simple, cost-effective alternative to traditional microscopes, offering flexible multicolor extended resolution fluorescence imaging and potential applications in multimodal imaging.</p>\",\"PeriodicalId\":15264,\"journal\":{\"name\":\"Journal of Biomedical Optics\",\"volume\":\"30 4\",\"pages\":\"046501\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11977515/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Biomedical Optics\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1117/1.JBO.30.4.046501\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/4/8 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biomedical Optics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1117/1.JBO.30.4.046501","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/8 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Significance: The multiplexing capabilities of fluorescence imaging are enhanced by its exceptional molecular specificity with diverse fluorescent probes, making it a powerful tool for studying complex biological structures, organization, and functions. Recent advances in super-resolution fluorescence microscopy have further revolutionized our ability to explore biology and related fields. However, current multicolor super-resolution fluorescence imaging systems often come with high costs and bulky designs.
Aim: We present a multicolor extended resolution fluorescence imaging system that uses light-emitting diode to simplify the optical path, make the design more compact, and reduce system costs.
Approach: This multicolor extended resolution fluorescence imaging system is based on structured illumination, utilizing a simple diffraction unit positioned between the light source and the sample in a wide-field microscope. Notably, this design could be easily integrated into standard widefield microscopes as a convenient add-on unit, enabling extended resolution imaging.
Results: Our system demonstrates concurrent extended resolved imaging of three-color microsphere beads and successfully showcases multicolor extended resolution fluorescence imaging of biological tissue samples, revealing intricate structural details.
Conclusions: This system provides a structurally simple, cost-effective alternative to traditional microscopes, offering flexible multicolor extended resolution fluorescence imaging and potential applications in multimodal imaging.
期刊介绍:
The Journal of Biomedical Optics publishes peer-reviewed papers on the use of modern optical technology for improved health care and biomedical research.