{"title":"野生迷迭香精油和提取物的GC/MS和LC组成分析：抗氧化、抗菌和抗炎活性评价","authors":"Nasma Mahboub, Inasse Cherfi, Salah Eddine Laouini, Abderrhmane Bouafia, Abir Benaissa, Khaoula Alia, Fahad Alharthi, Khansaa Al-Essa, Farid Menaa","doi":"10.1002/bmc.70084","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p><i>Rosmarinus officinalis</i> L. (rosemary) is a widely used medicinal plant known for its antioxidant, antimicrobial, and anti-inflammatory properties. This study evaluates the bioactive potential of its essential oil (EO), methanolic (ME), and aqueous (AE) extracts. GC-MS analysis identified α-pinene (21.37%), bornanone (12.73%), and eucalyptol (8.28%) as major EO components, while HPLC revealed ME's richness in salicylic acid (5.11 μg/mg) and rutin (0.43 μg/mg). Antioxidant activity, assessed via DPPH and FRAP assays, showed ME with the strongest radical scavenging capacity (IC<sub>50</sub> = 27.30 ± 2.4%) and reducing power (IC<sub>50</sub> = 90.88 ± 6.7%). Antimicrobial testing revealed EO as the most effective, particularly against <i>Staphylococcus aureus</i> (33 mm inhibition zone) and <i>Bacillus subtilis</i> (32 mm), while AE and ME exhibited moderate activity. <i>Pseudomonas aeruginosa</i> was resistant to all extracts. Additionally, AE demonstrated notable anti-inflammatory activity (IC<sub>50</sub> = 55.88 ± 1.02%). These findings highlight rosemary as a rich source of bioactive compounds with strong pharmacological potential, positioning ME as the best antioxidant, EO as the most potent antimicrobial, and AE as an effective anti-inflammatory agent.</p>\n </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"GC/MS and LC Composition Analysis of Essential Oil and Extracts From Wild Rosemary: Evaluation of Their Antioxidant, Antimicrobial, and Anti-Inflammatory Activities\",\"authors\":\"Nasma Mahboub, Inasse Cherfi, Salah Eddine Laouini, Abderrhmane Bouafia, Abir Benaissa, Khaoula Alia, Fahad Alharthi, Khansaa Al-Essa, Farid Menaa\",\"doi\":\"10.1002/bmc.70084\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p><i>Rosmarinus officinalis</i> L. (rosemary) is a widely used medicinal plant known for its antioxidant, antimicrobial, and anti-inflammatory properties. This study evaluates the bioactive potential of its essential oil (EO), methanolic (ME), and aqueous (AE) extracts. GC-MS analysis identified α-pinene (21.37%), bornanone (12.73%), and eucalyptol (8.28%) as major EO components, while HPLC revealed ME's richness in salicylic acid (5.11 μg/mg) and rutin (0.43 μg/mg). Antioxidant activity, assessed via DPPH and FRAP assays, showed ME with the strongest radical scavenging capacity (IC<sub>50</sub> = 27.30 ± 2.4%) and reducing power (IC<sub>50</sub> = 90.88 ± 6.7%). Antimicrobial testing revealed EO as the most effective, particularly against <i>Staphylococcus aureus</i> (33 mm inhibition zone) and <i>Bacillus subtilis</i> (32 mm), while AE and ME exhibited moderate activity. <i>Pseudomonas aeruginosa</i> was resistant to all extracts. Additionally, AE demonstrated notable anti-inflammatory activity (IC<sub>50</sub> = 55.88 ± 1.02%). These findings highlight rosemary as a rich source of bioactive compounds with strong pharmacological potential, positioning ME as the best antioxidant, EO as the most potent antimicrobial, and AE as an effective anti-inflammatory agent.</p>\\n </div>\",\"PeriodicalId\":8861,\"journal\":{\"name\":\"Biomedical Chromatography\",\"volume\":\"39 5\",\"pages\":\"\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2025-04-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical Chromatography\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/bmc.70084\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Chromatography","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/bmc.70084","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
GC/MS and LC Composition Analysis of Essential Oil and Extracts From Wild Rosemary: Evaluation of Their Antioxidant, Antimicrobial, and Anti-Inflammatory Activities
Rosmarinus officinalis L. (rosemary) is a widely used medicinal plant known for its antioxidant, antimicrobial, and anti-inflammatory properties. This study evaluates the bioactive potential of its essential oil (EO), methanolic (ME), and aqueous (AE) extracts. GC-MS analysis identified α-pinene (21.37%), bornanone (12.73%), and eucalyptol (8.28%) as major EO components, while HPLC revealed ME's richness in salicylic acid (5.11 μg/mg) and rutin (0.43 μg/mg). Antioxidant activity, assessed via DPPH and FRAP assays, showed ME with the strongest radical scavenging capacity (IC50 = 27.30 ± 2.4%) and reducing power (IC50 = 90.88 ± 6.7%). Antimicrobial testing revealed EO as the most effective, particularly against Staphylococcus aureus (33 mm inhibition zone) and Bacillus subtilis (32 mm), while AE and ME exhibited moderate activity. Pseudomonas aeruginosa was resistant to all extracts. Additionally, AE demonstrated notable anti-inflammatory activity (IC50 = 55.88 ± 1.02%). These findings highlight rosemary as a rich source of bioactive compounds with strong pharmacological potential, positioning ME as the best antioxidant, EO as the most potent antimicrobial, and AE as an effective anti-inflammatory agent.
期刊介绍：
Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.
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