{"title":"壳聚糖- pnipam金属-有机骨架的奥沙利铂和卡巴他赛双药递送工程：通往肝癌pH/热响应策略的精确途径","authors":"Conghuan Shen, Jianhua Li, Quanbao Zhang, Yifeng Tao, Ruidong Li, Zhenyu Ma, Zhengxin Wang","doi":"10.1007/s10924-025-03510-x","DOIUrl":null,"url":null,"abstract":"<div><p>A pH/thermo-responsive nanoframeworks has been engineered using ZIF-8 decorated with the chitosan (C)-poly(N-isopropylacrylamide) (C-PNIPAM) for the co-delivery of oxaliplatin (OXP) and cabazitaxel (CTX) in liver cancer cells. The chitosan PNIPAM nanoparticles (CPNPs) exhibited particle diameters of 205 nm and a surface charge of + 28 mV. The CTX and OXP loading contents in the CPNPs were 12.10% and 54.49%. NPs exhibited a pH-and thermo-responsive drug release, characterized by a continuous and extended-release. The anticancer activity demonstrated a notable synergistic effect of OXP@ZIF-8/CTX@CPNPs, resulting in HepG2 and Bel7402 cells IC<sub>50</sub> of 2.21 and 5.23 µg/mL. The resultant NPs were lower cytotoxic than OXP and CTX on non-cancerous NIH3T3 cell lines. OXP@ZIF-8/CTX@CPNPs shows enhanced cellular uptake in HepG2 cells. The apoptotic mode of cell death in OXP@ZIF-8/CTX@CPNPs treated cells was indicated by acridine orange/propidium iodide (AO/PI) staining techniques. 4′, 6-diamidino-2-phenylindole (DAPI) staining also confirmed nuclear changes such as fragmentation and condensation. Annexin V-FITC/Propidium iodide (PI) staining with flow cytometry showed that OXP@ZIF-8/CTX@CPNPs improved apoptosis in HepG2 cells. The study validated the superior efficacy of the engineered polymeric drugs integrated with metal-organic nanoframeworks in combinational treatment against liver cancer cell lines, exhibiting reduced adverse effects compared to free anticancer drugs (OXP and CTX).</p></div>","PeriodicalId":659,"journal":{"name":"Journal of Polymers and the Environment","volume":"33 5","pages":"2280 - 2299"},"PeriodicalIF":4.7000,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Engineering of Dual-drug Delivery of Oxaliplatin and Cabazitaxel Using Chitosan-PNIPAM Clacked Metal-organic Frameworks: A Path to Precision of pH/thermo Responsive Tactic to Liver cancer\",\"authors\":\"Conghuan Shen, Jianhua Li, Quanbao Zhang, Yifeng Tao, Ruidong Li, Zhenyu Ma, Zhengxin Wang\",\"doi\":\"10.1007/s10924-025-03510-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A pH/thermo-responsive nanoframeworks has been engineered using ZIF-8 decorated with the chitosan (C)-poly(N-isopropylacrylamide) (C-PNIPAM) for the co-delivery of oxaliplatin (OXP) and cabazitaxel (CTX) in liver cancer cells. The chitosan PNIPAM nanoparticles (CPNPs) exhibited particle diameters of 205 nm and a surface charge of + 28 mV. The CTX and OXP loading contents in the CPNPs were 12.10% and 54.49%. NPs exhibited a pH-and thermo-responsive drug release, characterized by a continuous and extended-release. The anticancer activity demonstrated a notable synergistic effect of OXP@ZIF-8/CTX@CPNPs, resulting in HepG2 and Bel7402 cells IC<sub>50</sub> of 2.21 and 5.23 µg/mL. The resultant NPs were lower cytotoxic than OXP and CTX on non-cancerous NIH3T3 cell lines. OXP@ZIF-8/CTX@CPNPs shows enhanced cellular uptake in HepG2 cells. The apoptotic mode of cell death in OXP@ZIF-8/CTX@CPNPs treated cells was indicated by acridine orange/propidium iodide (AO/PI) staining techniques. 4′, 6-diamidino-2-phenylindole (DAPI) staining also confirmed nuclear changes such as fragmentation and condensation. Annexin V-FITC/Propidium iodide (PI) staining with flow cytometry showed that OXP@ZIF-8/CTX@CPNPs improved apoptosis in HepG2 cells. The study validated the superior efficacy of the engineered polymeric drugs integrated with metal-organic nanoframeworks in combinational treatment against liver cancer cell lines, exhibiting reduced adverse effects compared to free anticancer drugs (OXP and CTX).</p></div>\",\"PeriodicalId\":659,\"journal\":{\"name\":\"Journal of Polymers and the Environment\",\"volume\":\"33 5\",\"pages\":\"2280 - 2299\"},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2025-02-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Polymers and the Environment\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10924-025-03510-x\",\"RegionNum\":3,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENGINEERING, ENVIRONMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Polymers and the Environment","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10924-025-03510-x","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
Engineering of Dual-drug Delivery of Oxaliplatin and Cabazitaxel Using Chitosan-PNIPAM Clacked Metal-organic Frameworks: A Path to Precision of pH/thermo Responsive Tactic to Liver cancer
A pH/thermo-responsive nanoframeworks has been engineered using ZIF-8 decorated with the chitosan (C)-poly(N-isopropylacrylamide) (C-PNIPAM) for the co-delivery of oxaliplatin (OXP) and cabazitaxel (CTX) in liver cancer cells. The chitosan PNIPAM nanoparticles (CPNPs) exhibited particle diameters of 205 nm and a surface charge of + 28 mV. The CTX and OXP loading contents in the CPNPs were 12.10% and 54.49%. NPs exhibited a pH-and thermo-responsive drug release, characterized by a continuous and extended-release. The anticancer activity demonstrated a notable synergistic effect of OXP@ZIF-8/CTX@CPNPs, resulting in HepG2 and Bel7402 cells IC50 of 2.21 and 5.23 µg/mL. The resultant NPs were lower cytotoxic than OXP and CTX on non-cancerous NIH3T3 cell lines. OXP@ZIF-8/CTX@CPNPs shows enhanced cellular uptake in HepG2 cells. The apoptotic mode of cell death in OXP@ZIF-8/CTX@CPNPs treated cells was indicated by acridine orange/propidium iodide (AO/PI) staining techniques. 4′, 6-diamidino-2-phenylindole (DAPI) staining also confirmed nuclear changes such as fragmentation and condensation. Annexin V-FITC/Propidium iodide (PI) staining with flow cytometry showed that OXP@ZIF-8/CTX@CPNPs improved apoptosis in HepG2 cells. The study validated the superior efficacy of the engineered polymeric drugs integrated with metal-organic nanoframeworks in combinational treatment against liver cancer cell lines, exhibiting reduced adverse effects compared to free anticancer drugs (OXP and CTX).
期刊介绍：
The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.
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