荧光探针检测羧酸酯酶水平对中药水煎剂疗效的影响

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-04-09 DOI:10.1016/j.saa.2025.126187

Jing-Yi Shi , Chao-Yue Wang , Yifan Xia , Meng-Ya Guo , Wen Yang , Yu-Shun Yang , Hai-Liang Zhu

{"title":"荧光探针检测羧酸酯酶水平对中药水煎剂疗效的影响","authors":"Jing-Yi Shi , Chao-Yue Wang , Yifan Xia , Meng-Ya Guo , Wen Yang , Yu-Shun Yang , Hai-Liang Zhu","doi":"10.1016/j.saa.2025.126187","DOIUrl":null,"url":null,"abstract":"<div><div>In this work, a fluorescent probe <strong>ImPI-CE</strong> with the imidazo-pyridin-isovanillin sub-structure was developed and studied for monitoring the Carboxylesterase (CE) level in colorectal cells on evaluating the Chinese medicine water decoction efficacy. The advantages of <strong>ImPI-CE</strong> included rapid response, high stability, high sensitivity, high selectivity, and reliable anti-interference. The most significant challenging point here was the biological efficacy-based test and the imaging in colorectal scenario which were both suitable for the international recognition of Chinese medicine water decoction. In particular, <strong>ImPI-CE</strong> achieved the stable monitoring of multiple Chinese medicine water decoction samples. It was able to imaging the CE level in both the inhibition and activation situations in living colorectal cells. <strong>ImPI-CE</strong> also realized the intracellular biological efficacy-based investigation of both the separate and combination Chinese medicine water decoction samples. The findings were beneficial for the international recognition of Chinese medicine.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"338 ","pages":"Article 126187"},"PeriodicalIF":4.3000,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A fluorescent probe for detecting carboxylesterase level on investigating Chinese medicine water decoction efficacy\",\"authors\":\"Jing-Yi Shi , Chao-Yue Wang , Yifan Xia , Meng-Ya Guo , Wen Yang , Yu-Shun Yang , Hai-Liang Zhu\",\"doi\":\"10.1016/j.saa.2025.126187\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>In this work, a fluorescent probe <strong>ImPI-CE</strong> with the imidazo-pyridin-isovanillin sub-structure was developed and studied for monitoring the Carboxylesterase (CE) level in colorectal cells on evaluating the Chinese medicine water decoction efficacy. The advantages of <strong>ImPI-CE</strong> included rapid response, high stability, high sensitivity, high selectivity, and reliable anti-interference. The most significant challenging point here was the biological efficacy-based test and the imaging in colorectal scenario which were both suitable for the international recognition of Chinese medicine water decoction. In particular, <strong>ImPI-CE</strong> achieved the stable monitoring of multiple Chinese medicine water decoction samples. It was able to imaging the CE level in both the inhibition and activation situations in living colorectal cells. <strong>ImPI-CE</strong> also realized the intracellular biological efficacy-based investigation of both the separate and combination Chinese medicine water decoction samples. The findings were beneficial for the international recognition of Chinese medicine.</div></div>\",\"PeriodicalId\":433,\"journal\":{\"name\":\"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy\",\"volume\":\"338 \",\"pages\":\"Article 126187\"},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2025-04-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1386142525004937\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"SPECTROSCOPY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1386142525004937","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SPECTROSCOPY","Score":null,"Total":0}

引用次数: 0

摘要

本研究开发了一种具有咪唑吡啶-异香兰素子结构的荧光探针ImPI-CE，并将其用于监测结直肠细胞中的羧酸酯酶（CE）水平，以评估中药水煎剂的疗效。ImPI-CE 的优点是反应快速、稳定性高、灵敏度高、选择性高、抗干扰性强。其中最重要的挑战点是基于生物药效的测试和结直肠场景成像，这两点都适合中药水煎剂的国际认可。其中，Impi-CE 实现了对多个中药水煎剂样品的稳定监测。它能对活体大肠细胞在抑制和激活状态下的 CE 水平进行成像。ImPI-CE还实现了对单独中药水煎剂样品和复方中药水煎剂样品进行基于细胞内生物功效的研究。这些研究结果有利于中药的国际认可。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A fluorescent probe for detecting carboxylesterase level on investigating Chinese medicine water decoction efficacy

查看原文本刊更多论文

A fluorescent probe for detecting carboxylesterase level on investigating Chinese medicine water decoction efficacy

In this work, a fluorescent probe ImPI-CE with the imidazo-pyridin-isovanillin sub-structure was developed and studied for monitoring the Carboxylesterase (CE) level in colorectal cells on evaluating the Chinese medicine water decoction efficacy. The advantages of ImPI-CE included rapid response, high stability, high sensitivity, high selectivity, and reliable anti-interference. The most significant challenging point here was the biological efficacy-based test and the imaging in colorectal scenario which were both suitable for the international recognition of Chinese medicine water decoction. In particular, ImPI-CE achieved the stable monitoring of multiple Chinese medicine water decoction samples. It was able to imaging the CE level in both the inhibition and activation situations in living colorectal cells. ImPI-CE also realized the intracellular biological efficacy-based investigation of both the separate and combination Chinese medicine water decoction samples. The findings were beneficial for the international recognition of Chinese medicine.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.