小叶Lippia Cham的抗真菌和抗膜活性。精油。（马鞭草科）对白色念珠菌和人体细胞的细胞毒性

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology Pub Date : 2025-03-30 DOI:10.1016/j.archoralbio.2025.106243

Jaqueline Oliveira Barreto , Larissa Alves da Silva , Luanna de Oliveira e Lima , Paula Lima Nogueira , Rafael Carlos Ferreira , Heivila Monique da Silva Alexandre , Anauara Lima e Silva , Juan Carlos Ramos Gonçalves , Marianna Vieira Sobral , Ricardo Dias de Castro , Yuri Mangueira do Nascimento , Josean Fechine Tavares , Walicyranison Plinio da Silva Rocha , Felipe Queiroga Sarmento Guerra

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引用次数: 0

摘要

目的研究小叶磷脂精油（LM-EO）对白色念珠菌的抑菌活性。设计通过体外实验检测LM-EO的抗念珠菌作用、抗生物膜活性、共聚焦显微镜观察到的对白色念珠菌生物膜荧光强度的影响、可能的作用机制以及对人角质形成细胞的毒性。考虑α = 5 %进行统计学分析。结果LM-EO的最小抑菌浓度（MIC）为128 ~ 256 µg/mL，最小杀真菌浓度（MFC）为256 ~ 1024 µg/mL。山梨醇存在时，MIC值没有变化，而麦角甾醇存在时，MIC值增加了4倍，表明LM-EO可能作用于细胞膜。这随后通过共聚焦显微镜观察使用碘化丙啶标记物。LM-EO在低浓度（256-2560 µg/mL）下使生物膜粘附率降低66-86 % (p <； 0.0001)；然而，它在人体细胞中显示出毒性。结论slm - eo可能通过麦角甾醇络合作用机制表现出杀真菌活性，但对人角质形成细胞具有细胞毒性。

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Antifungal and antibiofilm activity of Lippia microphylla Cham. essential oil. (Verbenaceae) on Candida albicans and human cell cytotoxicity

Objective

This study evaluated the antifungal activity of Lippia microphylla essential oil (LM-EO) on Candida albicans.

Design

In vitro assays were conducted to test LM-EO for its anti-Candida effects, antibiofilm activity, effects on the fluorescence intensity of Candida albicans biofilms observed via confocal microscopy, probable mechanism of action, and toxicity on human keratinocytes. Statistical analysis was performed considering α = 5 %.

Results

The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of LM-EO ranged from 128 µg/mL to 256 µg/mL and 256 µg/mL to 1024 µg/mL, respectively. MIC values did not change in the presence of sorbitol, whereas a 4-fold increase was observed in the presence of ergosterol, suggesting that LM-EO may act on the cell membrane. This was subsequently observed via confocal microscopy using propidium iodide markers. LM-EO reduced biofilm adherence by 66–86 % (p < 0.0001) at low concentrations (256–2560 µg/mL); however, it showed toxicity in human cells.

Conclusions

LM-EO exhibited fungicidal activity likely through a mechanism related to ergosterol complexation but demonstrated cytotoxicity to human keratinocytes.

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来源期刊

Archives of oral biology 医学-牙科与口腔外科

CiteScore

5.10

自引率

3.30%

发文量

177

审稿时长

26 days

期刊介绍： Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including: Cell and molecular biology Molecular genetics Immunology Pathogenesis Cellular microbiology Embryology Syndromology Forensic dentistry