巴西一家医院4年来患者中携带blakpc的大肠杆菌的描述

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2025-04-05 DOI:10.1016/j.diagmicrobio.2025.116833

Letícia Kalir Pradela , Tiago Casella , Fernanda Zani Manieri , Letícia Kellen de Andrade , Marlon do Valle Barroso , Nguyen Thi Khanh Nhu , Mark Andrew Schembri , Cristiano Gallina Moreira , Mara Corrêa Lelles Nogueira

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引用次数: 0

摘要

产碳青霉烯酶（KPC）的肺炎克雷伯菌大肠杆菌被世界卫生组织认定为具有公共卫生重要性的重要细菌性病原体。因此，需要进一步了解产生kpc的大肠杆菌的遗传特性。在这里，我们在巴西一家教学医院进行了一项回顾性研究，以描述40种美罗培宁耐药大肠杆菌的抗微生物药物耐药性、毒力和系统发育相关的基因组特征。所有分离株均携带blaKPC-2基因，但阿米卡星、替加环素、粘菌素、多粘菌素B和磷霉素表现出较好的活性。MLST分子分型共发现20种序列类型（STs），其中ST131为优势序列。全基因组测序确定Tn4401是blaKPC-2动员的机制，多种抗菌素抗性和毒力基因，致病系统群谱系的优势，以及属于同一st的基因组分组。kpc产生的大肠杆菌不是常见的病原体，但很少有治疗方案可用于潜在的毒力菌株。

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Description of blaKPC-carrying Escherichia coli in patients from a Brazilian hospital over a 4-year period

Klebsiella pneumoniae carbapenemase (KPC)-producing Escherichia coli are recognized by the World Health Organization as a critical group of bacterial priority pathogens of public health importance. Thus, increased understanding of the genetic characteristics of KPC-producing E. coli is required. Here, we performed a retrospective study in a Brazilian teaching-hospital to describe the genomic features linked to antimicrobial resistance, virulence, and phylogeny of 40 meropenem-resistant E. coli. All isolates carried the bla_KPC-2 gene, but amikacin, tigecycline, colistin, polymyxin B, and fosfomycin showed good activity. Molecular typing by MLST revealed 20 sequence types (STs), with a predominance of ST131. Whole-genome sequencing identified Tn4401 as a mechanism responsible for bla_KPC-2 mobilization, a variety of antimicrobial resistance and virulence genes, the predominance of pathogenic phylogroup lineages, and the grouping of genomes belonging to the same ST. KPC-producing E. coli is not a common pathogen, but few treatment alternatives are available against potentially virulent strains.

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.