{"title":"观赏贸易进口蓝螯虾(原螯虾)白斑综合征病毒的检测与鉴定","authors":"Naomi Falconnier , Adrien Izquierdo , Shannon Gray , Nanny Wenzlow , Kuttichantran Subramaniam","doi":"10.1016/j.jip.2025.108330","DOIUrl":null,"url":null,"abstract":"<div><div>White spot disease, caused by the white spot syndrome virus (WSSV), is a notifiable disease of decapod crustaceans, and international trade poses risks for its spread. Here, we describe an asymptomatic mortality event in blue crayfish (<em>Procambarus alleni</em>) imported from Thailand to the USA through the ornamental trade. WSSV was confirmed with histology, in situ hybridization, and real-time PCR. Next-generation sequencing and phylogenetic analysis revealed that this WSSV isolate was genetically distinct from the USA isolate and clustered with Asian isolates. This report exemplifies the poorly documented risk of disease spread through the ornamental trade.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"211 ","pages":"Article 108330"},"PeriodicalIF":2.4000,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Detection and characterization of white spot syndrome virus in imported blue crayfish (Procambarus alleni) from the ornamental trade\",\"authors\":\"Naomi Falconnier , Adrien Izquierdo , Shannon Gray , Nanny Wenzlow , Kuttichantran Subramaniam\",\"doi\":\"10.1016/j.jip.2025.108330\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>White spot disease, caused by the white spot syndrome virus (WSSV), is a notifiable disease of decapod crustaceans, and international trade poses risks for its spread. Here, we describe an asymptomatic mortality event in blue crayfish (<em>Procambarus alleni</em>) imported from Thailand to the USA through the ornamental trade. WSSV was confirmed with histology, in situ hybridization, and real-time PCR. Next-generation sequencing and phylogenetic analysis revealed that this WSSV isolate was genetically distinct from the USA isolate and clustered with Asian isolates. This report exemplifies the poorly documented risk of disease spread through the ornamental trade.</div></div>\",\"PeriodicalId\":16296,\"journal\":{\"name\":\"Journal of invertebrate pathology\",\"volume\":\"211 \",\"pages\":\"Article 108330\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-04-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of invertebrate pathology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0022201125000643\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ZOOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of invertebrate pathology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022201125000643","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ZOOLOGY","Score":null,"Total":0}
Detection and characterization of white spot syndrome virus in imported blue crayfish (Procambarus alleni) from the ornamental trade
White spot disease, caused by the white spot syndrome virus (WSSV), is a notifiable disease of decapod crustaceans, and international trade poses risks for its spread. Here, we describe an asymptomatic mortality event in blue crayfish (Procambarus alleni) imported from Thailand to the USA through the ornamental trade. WSSV was confirmed with histology, in situ hybridization, and real-time PCR. Next-generation sequencing and phylogenetic analysis revealed that this WSSV isolate was genetically distinct from the USA isolate and clustered with Asian isolates. This report exemplifies the poorly documented risk of disease spread through the ornamental trade.
期刊介绍:
The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates.
The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.