2018-2020年宾夕法尼亚州美国黑熊(美洲熊)微丝虫流行情况。

IF 1 4区 医学 Q4 PARASITOLOGY
Alia Mazzatosta, Christopher A Cleveland, Michael J Yabsley, Ellen Haynes, Hannah S Tiffin, Erika T Machtinger, Joshua B Johnson, Mark Ternent, Emily Carrollo, Justin D Brown
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引用次数: 0

摘要

摘要熊Dirofilaria ursi是美洲黑熊(Ursus americanus)的一种丝状线虫,由黑蝇(拟蝇科)传播。与熊的熊乳杆菌感染相关的明显临床疾病尚未报道;然而,寄生虫的成虫或幼虫(即微丝虫)阶段可以在皮下、腹部和胸部的结缔组织或血膜上偶然发现。在过去的60年里,在整个美国北部和加拿大的黑熊中都发现了熊耳球菌。然而,自2000年以来,很少进行调查,特别是在美国东北部,那里的黑熊数量在最近几十年里显著增加。在2018-2020年期间,研究人员在所有季节从宾夕法尼亚州收集了129只黑熊的血膜,检查了微丝虫。129只黑熊中有33只(25.6%)被鉴定出与熊丝虫形态一致的微丝虫。阳性血膜平均微丝蚴数27.4个(范围1 ~ 282个)。年龄、性别和季节对黑熊的Dirofilaria感染检测无显著影响。采用聚合酶链反应(PCR)和18S rRNA基因区测序对11份血膜含微丝虫(平均17.3微丝虫/膜;范围:1-55)。11份全血样本中有10份Dirofilaria PCR阳性,序列与GenBank中Dirofilaria repens的序列高度相似。这项研究的结果与之前熊博士对北美黑熊的调查结果一致,表明芽孢杆菌感染在宾夕法尼亚州的黑熊中很常见,但似乎与显性疾病无关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
PREVALENCE OF DIROFILARIA MICROFILARIA IN AMERICAN BLACK BEARS (URSUS AMERICANUS) IN PENNSYLVANIA, 2018-2020.

Abstract: Dirofilaria ursi is a filarial nematode of American black bears (Ursus americanus) transmitted by black flies (Simuliidae). Overt clinical disease associated with D. ursi infection of bears has not been reported; however, adult or larval (i.e., microfilarial) stages of the parasite can be found incidentally in the subcutis, connective tissues of the abdomen and thorax, or on blood films. Over the last 60 yr, D. ursi has been reported in black bears throughout the northern United States and Canada. However, very few surveys have been conducted since 2000, particularly in the northeastern United States where black bear populations have expanded significantly over recent decades. During 2018-2020, blood films collected from 129 black bears from Pennsylvania during all seasons were examined for microfilariae. Microfilariae that were morphologically consistent with D. ursi were identified in 33 (25.6%) of the 129 sampled black bears. The average number of microfilariae per positive blood film was 27.4 (range: 1-282). Age, sex, and season did not have a significant effect on detection of Dirofilaria infection in black bears. Molecular identification of Dirofilaria species via polymerase chain reaction (PCR) and sequencing of the 18S rRNA gene region was conducted for 11 whole blood samples whose blood films contained microfilariae (average of 17.3 microfilariae/film; range: 1-55). Ten of the 11 whole blood samples were PCR positive for Dirofilaria, and the sequences were highly similar to sequences of Dirofilaria repens available in GenBank. The results of this study are consistent with those of previous D. ursi surveys in North American black bears and indicate that Dirofilaria infection is common in black bears in Pennsylvania but does not appear to be associated with overt disease.

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来源期刊
Journal of Parasitology
Journal of Parasitology 医学-寄生虫学
CiteScore
2.10
自引率
7.70%
发文量
60
审稿时长
2 months
期刊介绍: The Journal of Parasitology is the official peer-reviewed journal of the American Society of Parasitologists (ASP). The journal publishes original research covering helminths, protozoa, and other parasitic organisms and serves scientific professionals in microbiology, immunology, veterinary science, pathology, and public health. Journal content includes original research articles, brief research notes, announcements of the Society, and book reviews. Articles are subdivided by topic for ease of reference and range from behavior and pathogenesis to systematics and epidemiology. The journal is published continuously online with one full volume printed at the end of each year.
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