短喙针鼹(Tachyglossus aculeatus)哺乳期微生物群的变化。

IF 3.5 3区 生物学 Q2 MICROBIOLOGY
Isabella Wilson, Tahlia Perry, Raphael Eisenhofer, Peggy Rismiller, Michelle Shaw, Frank Grutzner
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引用次数: 0

摘要

单目动物和有袋动物的发育特点是妊娠期短,幼崽在发育早期就暴露在环境中,并在育儿袋、假育儿袋或洞穴中长时间哺乳。这些晚熟的幼体缺乏功能性适应性免疫系统,这引发了关于它们如何在微生物丰富的环境中生存的问题。先前对有袋动物育儿袋的研究已经揭示了哺乳期间育儿袋微生物组成的变化,但在单孔目动物中没有相关信息。我们研究了针鼹假育儿袋微生物群(n = 22)在生殖周期不同阶段的变化,以及野生和动物园管理动物之间是否存在差异。利用16S rRNA基因测序的元分类分析显示,哺乳期间假育儿袋微生物群落发生了巨大的变化,与非繁殖季节或求偶和交配期间的样本相比,其分类组成存在显著差异。这表明针鼹假育儿袋环境在哺乳期间发生变化,以适应缺乏功能性适应性免疫系统的幼鼠。此外,圈养并没有发现对假袋微生物群有显著影响。本研究开创了单孔目动物育儿袋微生物群研究的先河,为针鼹繁殖提供了新的生物学信息,也可能为未来的繁殖计划提供有关圈养管理影响的信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microbiota changes in lactation in the short-beaked echidna (Tachyglossus aculeatus).

Monotreme and marsupial development is characterised by a short gestation, with young exposed to the environment at an early developmental stage and supported by a long lactation in the pouch, pseudo-pouch, or burrow. The lack of a functional adaptive immune system in these altricial young raises questions about how they survive in a microbe-rich environment. Previous studies on marsupial pouches have revealed changes to pouch microbe composition during lactation, but no information is available in monotremes. We investigated changes in the echidna pseudo-pouch microbiota (n = 22) during different stages of the reproductive cycle and whether this differs between wild and zoo-managed animals. Metataxonomic profiling using 16S rRNA gene sequencing revealed that pseudo-pouch microbial communities undergo dramatic changes during lactation, with significant differences in taxonomic composition compared with samples taken outside of breeding season or during courtship and mating. This suggests that the echidna pseudo-pouch environment changes during lactation to accommodate young that lack a functional adaptive immune system. Furthermore, captivity was not found to have a significant effect on pseudo-pouch microbiota. This study pioneers pouch microbiota research in monotremes, provides new biological information on echidna reproduction, and may also provide information about the effects of captive management to inform breeding programs in the future.

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来源期刊
FEMS microbiology ecology
FEMS microbiology ecology 生物-微生物学
CiteScore
7.50
自引率
2.40%
发文量
132
审稿时长
3 months
期刊介绍: FEMS Microbiology Ecology aims to ensure efficient publication of high-quality papers that are original and provide a significant contribution to the understanding of microbial ecology. The journal contains Research Articles and MiniReviews on fundamental aspects of the ecology of microorganisms in natural soil, aquatic and atmospheric habitats, including extreme environments, and in artificial or managed environments. Research papers on pure cultures and in the areas of plant pathology and medical, food or veterinary microbiology will be published where they provide valuable generic information on microbial ecology. Papers can deal with culturable and non-culturable forms of any type of microorganism: bacteria, archaea, filamentous fungi, yeasts, protozoa, cyanobacteria, algae or viruses. In addition, the journal will publish Perspectives, Current Opinion and Controversy Articles, Commentaries and Letters to the Editor on topical issues in microbial ecology. - Application of ecological theory to microbial ecology - Interactions and signalling between microorganisms and with plants and animals - Interactions between microorganisms and their physicochemical enviornment - Microbial aspects of biogeochemical cycles and processes - Microbial community ecology - Phylogenetic and functional diversity of microbial communities - Evolutionary biology of microorganisms
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