双壳细胞中OsHV-1启动子驱动的转基因表达:egfp标记的致癌基因H-RasV12的体外研究

IF 2.7 3区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES
Soumya Balakrishnan, Ambadi Kannan Maliyekkal Sajeevan, R Namitha, M R Sunitha, A Muneer, I S Bright Singh, Jayesh Puthumana
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引用次数: 0

摘要

双壳类是可采掘的物种,支持可持续水产养殖和循环生物经济原则。生物技术干预可以提高双壳类水产养殖的生产力。例如,转基因表达促进了基因功能分析,从而能够鉴定用于水产养殖的双壳类的理想性状。转基因表达也应用于双壳类细胞系的开发,从而支持病理学研究、生态毒理学和水产养殖环境质量评估,并推进养殖海产品技术(与可持续发展目标一致)。在这项研究中,测试了合成的OsHV-1 (ostreid herpesvirus 1)启动子在不同细胞培养系统中驱动转基因表达的功效,包括双壳动物麦芽麦的原代细胞培养。我们还分析了OsHV-1启动子控制下带有增强绿色荧光蛋白(EGFP)标记的致癌基因H-RasV12的表达。结果表明,OsHV-1启动子比广泛使用的巨细胞病毒启动子具有更好的活性,这突出了合成启动子在软体动物细胞中转基因表达的潜力。此外,该研究表明,优化基因传递方法和整合多种致癌基因可以增强软体动物细胞的转化,为开发双壳类细胞系作为水生生物技术的有价值工具铺平了道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
OsHV-1 Promoter-driven Transgene Expression in Bivalve Cells: An In Vitro Study with an EGFP-tagged Oncogenic H-RasV12.

Bivalves, being extractive species, support sustainable aquaculture and the principles of a circular bioeconomy. Biotechnological interventions can improve bivalve aquaculture productivity. For example, transgene expression facilitates gene function analysis which enables the identification of desirable traits in bivalves for aquaculture. Transgene expression also finds applications in the development of bivalve cell lines that, in turn, support pathology research, eco-toxicology and aquaculture environment quality assessment, and advancing cultivated seafood technology (aligned with sustainability goals). In this study, the efficacy of a synthesized OsHV-1 (ostreid herpesvirus 1) promoter in driving transgene expression across diverse cell culture systems was tested, including primary cell cultures from the bivalve Magallana bilineata. The expression of the oncogenic H-RasV12, tagged with an enhanced green fluorescent protein (EGFP), under the control of the OsHV-1 promoter was also analyzed. The results showed that the OsHV-1 promoter exhibited better activity than the widely used CMV (cytomegalovirus) promoter, highlighting the potential of synthesized promoters for transgene expression in molluscan cells. Furthermore, the study suggests that optimizing gene delivery methods and incorporating multiple oncogenes could enhance molluscan cell transformation, paving the way for developing bivalve cell lines as valuable tools in aquatic biotechnology.

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来源期刊
Environmental Management
Environmental Management 环境科学-环境科学
CiteScore
6.20
自引率
2.90%
发文量
178
审稿时长
12 months
期刊介绍: Environmental Management offers research and opinions on use and conservation of natural resources, protection of habitats and control of hazards, spanning the field of environmental management without regard to traditional disciplinary boundaries. The journal aims to improve communication, making ideas and results from any field available to practitioners from other backgrounds. Contributions are drawn from biology, botany, chemistry, climatology, ecology, ecological economics, environmental engineering, fisheries, environmental law, forest sciences, geosciences, information science, public affairs, public health, toxicology, zoology and more. As the principal user of nature, humanity is responsible for ensuring that its environmental impacts are benign rather than catastrophic. Environmental Management presents the work of academic researchers and professionals outside universities, including those in business, government, research establishments, and public interest groups, presenting a wide spectrum of viewpoints and approaches.
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