Soumya Balakrishnan, Ambadi Kannan Maliyekkal Sajeevan, R Namitha, M R Sunitha, A Muneer, I S Bright Singh, Jayesh Puthumana
{"title":"双壳细胞中OsHV-1启动子驱动的转基因表达:egfp标记的致癌基因H-RasV12的体外研究","authors":"Soumya Balakrishnan, Ambadi Kannan Maliyekkal Sajeevan, R Namitha, M R Sunitha, A Muneer, I S Bright Singh, Jayesh Puthumana","doi":"10.1007/s00267-025-02159-5","DOIUrl":null,"url":null,"abstract":"<p><p>Bivalves, being extractive species, support sustainable aquaculture and the principles of a circular bioeconomy. Biotechnological interventions can improve bivalve aquaculture productivity. For example, transgene expression facilitates gene function analysis which enables the identification of desirable traits in bivalves for aquaculture. Transgene expression also finds applications in the development of bivalve cell lines that, in turn, support pathology research, eco-toxicology and aquaculture environment quality assessment, and advancing cultivated seafood technology (aligned with sustainability goals). In this study, the efficacy of a synthesized OsHV-1 (ostreid herpesvirus 1) promoter in driving transgene expression across diverse cell culture systems was tested, including primary cell cultures from the bivalve Magallana bilineata. The expression of the oncogenic H-RasV12, tagged with an enhanced green fluorescent protein (EGFP), under the control of the OsHV-1 promoter was also analyzed. The results showed that the OsHV-1 promoter exhibited better activity than the widely used CMV (cytomegalovirus) promoter, highlighting the potential of synthesized promoters for transgene expression in molluscan cells. Furthermore, the study suggests that optimizing gene delivery methods and incorporating multiple oncogenes could enhance molluscan cell transformation, paving the way for developing bivalve cell lines as valuable tools in aquatic biotechnology.</p>","PeriodicalId":543,"journal":{"name":"Environmental Management","volume":" ","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"OsHV-1 Promoter-driven Transgene Expression in Bivalve Cells: An In Vitro Study with an EGFP-tagged Oncogenic H-RasV12.\",\"authors\":\"Soumya Balakrishnan, Ambadi Kannan Maliyekkal Sajeevan, R Namitha, M R Sunitha, A Muneer, I S Bright Singh, Jayesh Puthumana\",\"doi\":\"10.1007/s00267-025-02159-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Bivalves, being extractive species, support sustainable aquaculture and the principles of a circular bioeconomy. Biotechnological interventions can improve bivalve aquaculture productivity. For example, transgene expression facilitates gene function analysis which enables the identification of desirable traits in bivalves for aquaculture. Transgene expression also finds applications in the development of bivalve cell lines that, in turn, support pathology research, eco-toxicology and aquaculture environment quality assessment, and advancing cultivated seafood technology (aligned with sustainability goals). In this study, the efficacy of a synthesized OsHV-1 (ostreid herpesvirus 1) promoter in driving transgene expression across diverse cell culture systems was tested, including primary cell cultures from the bivalve Magallana bilineata. The expression of the oncogenic H-RasV12, tagged with an enhanced green fluorescent protein (EGFP), under the control of the OsHV-1 promoter was also analyzed. The results showed that the OsHV-1 promoter exhibited better activity than the widely used CMV (cytomegalovirus) promoter, highlighting the potential of synthesized promoters for transgene expression in molluscan cells. 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OsHV-1 Promoter-driven Transgene Expression in Bivalve Cells: An In Vitro Study with an EGFP-tagged Oncogenic H-RasV12.
Bivalves, being extractive species, support sustainable aquaculture and the principles of a circular bioeconomy. Biotechnological interventions can improve bivalve aquaculture productivity. For example, transgene expression facilitates gene function analysis which enables the identification of desirable traits in bivalves for aquaculture. Transgene expression also finds applications in the development of bivalve cell lines that, in turn, support pathology research, eco-toxicology and aquaculture environment quality assessment, and advancing cultivated seafood technology (aligned with sustainability goals). In this study, the efficacy of a synthesized OsHV-1 (ostreid herpesvirus 1) promoter in driving transgene expression across diverse cell culture systems was tested, including primary cell cultures from the bivalve Magallana bilineata. The expression of the oncogenic H-RasV12, tagged with an enhanced green fluorescent protein (EGFP), under the control of the OsHV-1 promoter was also analyzed. The results showed that the OsHV-1 promoter exhibited better activity than the widely used CMV (cytomegalovirus) promoter, highlighting the potential of synthesized promoters for transgene expression in molluscan cells. Furthermore, the study suggests that optimizing gene delivery methods and incorporating multiple oncogenes could enhance molluscan cell transformation, paving the way for developing bivalve cell lines as valuable tools in aquatic biotechnology.
期刊介绍:
Environmental Management offers research and opinions on use and conservation of natural resources, protection of habitats and control of hazards, spanning the field of environmental management without regard to traditional disciplinary boundaries. The journal aims to improve communication, making ideas and results from any field available to practitioners from other backgrounds. Contributions are drawn from biology, botany, chemistry, climatology, ecology, ecological economics, environmental engineering, fisheries, environmental law, forest sciences, geosciences, information science, public affairs, public health, toxicology, zoology and more.
As the principal user of nature, humanity is responsible for ensuring that its environmental impacts are benign rather than catastrophic. Environmental Management presents the work of academic researchers and professionals outside universities, including those in business, government, research establishments, and public interest groups, presenting a wide spectrum of viewpoints and approaches.