{"title":"采用延迟补料分批同时糖化发酵的方法强化木糖发酵,最大限度地提高石灰预处理麦秸的净能量增益","authors":"Wan-ying Qiu, Chengyu Dong, Jing-jing Guo, Bo Xia, Lujiang Xu, Zhen Fang","doi":"10.1016/j.biombioe.2025.107865","DOIUrl":null,"url":null,"abstract":"<div><div>Delayed fed-batch simultaneous saccharification and fermentation (SSF) of lime-pretreated wheat straw was carried out with a wild type yeast (<em>i.e., Candida shehatae</em>) to produce high titer ethanol. Calcium ions in lime-pretreated wheat straw were precipitated by <em>pH</em> controlling agent (<em>i.e.,</em> sulfuric acid) to reduce inhibition of inorganic ions on yeast fermentation. It was found that ethanol concentration (∼35 g L<sup>−1</sup>) did not increase as solid loading of substrate increased from 15 % to 20 % in batch SSF. However, in delayed fed-batch SSF, the feeding time of substrate (96 h) was postponed until after the inoculation time (18 h) resulting in a 74 % reduction in residual xylose concentration and a 54.3 % increase in ethanol concentration (54 g L<sup>−1</sup>). Compared to batch SSF, separation energy of ethanol was decreased by 32.3 % to 977.3 MJ/ton wheat straw in delayed fed-batch SSF, and net energy gain was increased by 20.1 % to 3525.7 MJ/ton wheat straw.</div></div>","PeriodicalId":253,"journal":{"name":"Biomass & Bioenergy","volume":"198 ","pages":"Article 107865"},"PeriodicalIF":5.8000,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enhancing xylose fermentation to maximize net energy gain of lime-pretreated wheat straw by delayed fed-batch simultaneous saccharification and fermentation\",\"authors\":\"Wan-ying Qiu, Chengyu Dong, Jing-jing Guo, Bo Xia, Lujiang Xu, Zhen Fang\",\"doi\":\"10.1016/j.biombioe.2025.107865\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Delayed fed-batch simultaneous saccharification and fermentation (SSF) of lime-pretreated wheat straw was carried out with a wild type yeast (<em>i.e., Candida shehatae</em>) to produce high titer ethanol. Calcium ions in lime-pretreated wheat straw were precipitated by <em>pH</em> controlling agent (<em>i.e.,</em> sulfuric acid) to reduce inhibition of inorganic ions on yeast fermentation. It was found that ethanol concentration (∼35 g L<sup>−1</sup>) did not increase as solid loading of substrate increased from 15 % to 20 % in batch SSF. However, in delayed fed-batch SSF, the feeding time of substrate (96 h) was postponed until after the inoculation time (18 h) resulting in a 74 % reduction in residual xylose concentration and a 54.3 % increase in ethanol concentration (54 g L<sup>−1</sup>). Compared to batch SSF, separation energy of ethanol was decreased by 32.3 % to 977.3 MJ/ton wheat straw in delayed fed-batch SSF, and net energy gain was increased by 20.1 % to 3525.7 MJ/ton wheat straw.</div></div>\",\"PeriodicalId\":253,\"journal\":{\"name\":\"Biomass & Bioenergy\",\"volume\":\"198 \",\"pages\":\"Article 107865\"},\"PeriodicalIF\":5.8000,\"publicationDate\":\"2025-04-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomass & Bioenergy\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0961953425002764\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURAL ENGINEERING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomass & Bioenergy","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0961953425002764","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURAL ENGINEERING","Score":null,"Total":0}
引用次数: 0
摘要
利用野生型酵母(即假丝酵母)对石灰预处理的小麦秸秆进行延迟补料间歇同步糖化发酵(SSF),以生产高效价乙醇。采用pH控制剂(硫酸)沉淀石灰预处理麦秸中的钙离子,降低无机离子对酵母发酵的抑制作用。结果发现,随着底物固载量从15%增加到20%,乙醇浓度(~ 35 g L−1)并没有增加。然而,在延迟分批进料的SSF中,底物的进料时间(96 h)推迟到接种时间(18 h)之后,导致残留木糖浓度降低74%,乙醇浓度增加54.3% (54 g L−1)。延迟进料间歇式SSF与间歇式SSF相比,乙醇的分离能为977.3 MJ/t麦秸,降低了32.3%;净能增益为3525.7 MJ/t麦秸,提高了20.1%。
Enhancing xylose fermentation to maximize net energy gain of lime-pretreated wheat straw by delayed fed-batch simultaneous saccharification and fermentation
Delayed fed-batch simultaneous saccharification and fermentation (SSF) of lime-pretreated wheat straw was carried out with a wild type yeast (i.e., Candida shehatae) to produce high titer ethanol. Calcium ions in lime-pretreated wheat straw were precipitated by pH controlling agent (i.e., sulfuric acid) to reduce inhibition of inorganic ions on yeast fermentation. It was found that ethanol concentration (∼35 g L−1) did not increase as solid loading of substrate increased from 15 % to 20 % in batch SSF. However, in delayed fed-batch SSF, the feeding time of substrate (96 h) was postponed until after the inoculation time (18 h) resulting in a 74 % reduction in residual xylose concentration and a 54.3 % increase in ethanol concentration (54 g L−1). Compared to batch SSF, separation energy of ethanol was decreased by 32.3 % to 977.3 MJ/ton wheat straw in delayed fed-batch SSF, and net energy gain was increased by 20.1 % to 3525.7 MJ/ton wheat straw.
期刊介绍:
Biomass & Bioenergy is an international journal publishing original research papers and short communications, review articles and case studies on biological resources, chemical and biological processes, and biomass products for new renewable sources of energy and materials.
The scope of the journal extends to the environmental, management and economic aspects of biomass and bioenergy.
Key areas covered by the journal:
• Biomass: sources, energy crop production processes, genetic improvements, composition. Please note that research on these biomass subjects must be linked directly to bioenergy generation.
• Biological Residues: residues/rests from agricultural production, forestry and plantations (palm, sugar etc), processing industries, and municipal sources (MSW). Papers on the use of biomass residues through innovative processes/technological novelty and/or consideration of feedstock/system sustainability (or unsustainability) are welcomed. However waste treatment processes and pollution control or mitigation which are only tangentially related to bioenergy are not in the scope of the journal, as they are more suited to publications in the environmental arena. Papers that describe conventional waste streams (ie well described in existing literature) that do not empirically address ''new'' added value from the process are not suitable for submission to the journal.
• Bioenergy Processes: fermentations, thermochemical conversions, liquid and gaseous fuels, and petrochemical substitutes
• Bioenergy Utilization: direct combustion, gasification, electricity production, chemical processes, and by-product remediation
• Biomass and the Environment: carbon cycle, the net energy efficiency of bioenergy systems, assessment of sustainability, and biodiversity issues.