Integrated electrochemical sensing platform based on Au/MXene/PU three-dimensional scaffold for real-time monitoring of H2O2 release from cells under drug stimulation

Compared with traditional two-dimensional (2D) culture systems, three-dimensional (3D) culture systems can better simulate the physiological environment of cells in vivo, providing more accurate and real-time data. Although many reported 3D culture scaffolds can simulate the in vivo microenvironment, their use in real-time electrochemical sensing still limited by their insulating and non-compressible nature. In this research, we developed a simple and efficient way for preparing 3D cell culture-integrated electrochemical sensing platform for real-time monitoring of hydrogen peroxide (H₂O₂) release from cells under drug stimulation. The 3D integrated platform was a polyurethane (PU) scaffold modified MXene and gold nanoparticles (Au NPs) (Au/MXene/PU). The prepared Au/MXene/PU scaffold showed excellent electrocatalytic activity for H₂O₂ with a detection linear range of 0.1 to 100 μM. Furthermore, MDA-MB-231 cells were cultured on the 3D Au/MXene/PU scaffold and H₂O₂ released by cells were in situ monitored. These results indicate that the 3D Au/MXene/PU scaffold exhibits excellent potential applications in cell in vivo research cancer-related disease diagnosis and drug screening.