Yujie Cai, Rong Qiu, Qin Huang, Weinan Lai, Yipeng Han, Xiaoxi Lu, Jiayu Qin, Qingqing Ouyang, Min Yang
{"title":"Circ_0088200 可作为 miR-127-5p 的海绵，促进类风湿性关节炎成纤维细胞样滑膜细胞的迁移和侵袭。","authors":"Yujie Cai, Rong Qiu, Qin Huang, Weinan Lai, Yipeng Han, Xiaoxi Lu, Jiayu Qin, Qingqing Ouyang, Min Yang","doi":"10.1515/rir-2025-0002","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Circular RNAs (circRNAs) play a crucial role in the development of various diseases. However, few studies have investigated the role of circRNAs in rheumatoid arthritis (RA). Herein, we aimed to identified the novel circRNAs involved in the migration and invasion of RA fibroblast-like synoviocytes (RA-FLS).</p><p><strong>Methods: </strong>The RA-FLS were isolated from the synovial membrane of patients with RA. The CircRNA profile was screened by CircRNA microarray analysis. <i>Circ_0088200</i> and miR-127-5p expression levels were detected using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The protein level of matrix metalloproteinase 1 (MMP1) was evaluated by western blotting. Wound healing and Transwell assays were performed to analyze the migration and invasion of RA-FLS. RNA immunoprecipitation (RIP) and dual-luciferase reporter assays were used to validate the interaction between <i>Circ_0088200</i> and miR-127-5p. Collagen-induced arthritis (CIA) mouse models were established to evaluate the role of <i>Circ_0088200</i> in the development of arthritis <i>in vivo</i>.</p><p><strong>Results: </strong><i>Circ_0088200</i> was highly expressed in RA-FLS compared with osteoarthritis fibroblast-like synoviocytes (OAFLS) and correlated positively with the disease activity score in 28 joints. Inhibition of <i>Circ_0088200</i> suppressed the migration and invasion of RA-FLS. Conversely, overexpression of <i>Circ_0088200</i> significant promoted the migration and invasion of RA-FLS. Mechanistically, <i>Circ_0088200</i> functions as a sponge for miR-127-5p and relieve its repressive effect on <i>MMP1</i>, thereby promoting the migration and invasion of RA-FLS. Importantly, intra-articular injection of Adenoassociated virus expressing <i>Circ_0088200</i> significantly increased the severity of arthritis in mice with CIA.</p><p><strong>Conclusion: </strong><i>Circ_0088200</i> promotes the migration and invasion of RA-FLS by sponging miR-127-5p. Thus <i>Circ_0088200</i> is a potential therapeutic target for RA.</p>","PeriodicalId":74736,"journal":{"name":"Rheumatology and immunology research","volume":"6 1","pages":"7-20"},"PeriodicalIF":0.0000,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966198/pdf/","citationCount":"0","resultStr":"{\"title\":\"<i>Circ_0088200</i> acts as a sponge for miR-127-5p to promote the migration and invasion of rheumatoid arthritis fibroblast-like synoviocytes.\",\"authors\":\"Yujie Cai, Rong Qiu, Qin Huang, Weinan Lai, Yipeng Han, Xiaoxi Lu, Jiayu Qin, Qingqing Ouyang, Min Yang\",\"doi\":\"10.1515/rir-2025-0002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Circular RNAs (circRNAs) play a crucial role in the development of various diseases. However, few studies have investigated the role of circRNAs in rheumatoid arthritis (RA). Herein, we aimed to identified the novel circRNAs involved in the migration and invasion of RA fibroblast-like synoviocytes (RA-FLS).</p><p><strong>Methods: </strong>The RA-FLS were isolated from the synovial membrane of patients with RA. The CircRNA profile was screened by CircRNA microarray analysis. <i>Circ_0088200</i> and miR-127-5p expression levels were detected using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The protein level of matrix metalloproteinase 1 (MMP1) was evaluated by western blotting. Wound healing and Transwell assays were performed to analyze the migration and invasion of RA-FLS. RNA immunoprecipitation (RIP) and dual-luciferase reporter assays were used to validate the interaction between <i>Circ_0088200</i> and miR-127-5p. Collagen-induced arthritis (CIA) mouse models were established to evaluate the role of <i>Circ_0088200</i> in the development of arthritis <i>in vivo</i>.</p><p><strong>Results: </strong><i>Circ_0088200</i> was highly expressed in RA-FLS compared with osteoarthritis fibroblast-like synoviocytes (OAFLS) and correlated positively with the disease activity score in 28 joints. Inhibition of <i>Circ_0088200</i> suppressed the migration and invasion of RA-FLS. Conversely, overexpression of <i>Circ_0088200</i> significant promoted the migration and invasion of RA-FLS. Mechanistically, <i>Circ_0088200</i> functions as a sponge for miR-127-5p and relieve its repressive effect on <i>MMP1</i>, thereby promoting the migration and invasion of RA-FLS. Importantly, intra-articular injection of Adenoassociated virus expressing <i>Circ_0088200</i> significantly increased the severity of arthritis in mice with CIA.</p><p><strong>Conclusion: </strong><i>Circ_0088200</i> promotes the migration and invasion of RA-FLS by sponging miR-127-5p. Thus <i>Circ_0088200</i> is a potential therapeutic target for RA.</p>\",\"PeriodicalId\":74736,\"journal\":{\"name\":\"Rheumatology and immunology research\",\"volume\":\"6 1\",\"pages\":\"7-20\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-04-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966198/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Rheumatology and immunology research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1515/rir-2025-0002\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/3/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rheumatology and immunology research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/rir-2025-0002","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/3/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
Circ_0088200 acts as a sponge for miR-127-5p to promote the migration and invasion of rheumatoid arthritis fibroblast-like synoviocytes.
Background: Circular RNAs (circRNAs) play a crucial role in the development of various diseases. However, few studies have investigated the role of circRNAs in rheumatoid arthritis (RA). Herein, we aimed to identified the novel circRNAs involved in the migration and invasion of RA fibroblast-like synoviocytes (RA-FLS).
Methods: The RA-FLS were isolated from the synovial membrane of patients with RA. The CircRNA profile was screened by CircRNA microarray analysis. Circ_0088200 and miR-127-5p expression levels were detected using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The protein level of matrix metalloproteinase 1 (MMP1) was evaluated by western blotting. Wound healing and Transwell assays were performed to analyze the migration and invasion of RA-FLS. RNA immunoprecipitation (RIP) and dual-luciferase reporter assays were used to validate the interaction between Circ_0088200 and miR-127-5p. Collagen-induced arthritis (CIA) mouse models were established to evaluate the role of Circ_0088200 in the development of arthritis in vivo.
Results: Circ_0088200 was highly expressed in RA-FLS compared with osteoarthritis fibroblast-like synoviocytes (OAFLS) and correlated positively with the disease activity score in 28 joints. Inhibition of Circ_0088200 suppressed the migration and invasion of RA-FLS. Conversely, overexpression of Circ_0088200 significant promoted the migration and invasion of RA-FLS. Mechanistically, Circ_0088200 functions as a sponge for miR-127-5p and relieve its repressive effect on MMP1, thereby promoting the migration and invasion of RA-FLS. Importantly, intra-articular injection of Adenoassociated virus expressing Circ_0088200 significantly increased the severity of arthritis in mice with CIA.
Conclusion: Circ_0088200 promotes the migration and invasion of RA-FLS by sponging miR-127-5p. Thus Circ_0088200 is a potential therapeutic target for RA.
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