99mTc标记的蛋壳制备的小于50nm羟基磷灰石的放射性药物特性及其在兔体内的生物分布。

IF 0.9 4区 医学 Q4 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
Hellenic journal of nuclear medicine Pub Date : 2025-01-01 Epub Date: 2025-04-07 DOI:10.1967/s002449912787
Aziz Gültekin, Ayşe Uğur, Mine Sulak, Samiye Demirezen, Doğangün Yüksel
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引用次数: 0

摘要

目的:纳米粒子放射标记在个性化治疗和治疗应用中具有潜在的益处,近年来已被提上议程。羟基磷灰石纳米颗粒(HANP)是一种具有生物相容性的纳米颗粒,与骨组织具有很大的相似性。不同纳米尺寸羟基磷灰石分子的不同生物分布特性可能为其应用创造新的机会,特别是在骨成像和骨肿瘤治疗方面。本研究旨在研究用锝-99m (99mTc)标记蛋壳中小于50纳米的羟基磷灰石分子及其在家兔体内的分布。材料和方法:利用扫描电镜(SEM)、能量色散x射线(EDX)和x射线衍射(XRD)对蛋壳中获得的纳米羟基磷灰石颗粒进行了表征。用99mTc放射性核素对小于50nm的HANP进行放射性标记,考察标记产物的稳定性和在家兔体内的生物分布。结果:99mTc-HANP的放射化学纯度为96%。99mTc标记的HANP的体外稳定性长达12小时,并在盐水中显示出良好的体外稳定性。在6小时成像期间,锝-99m- hanp在体内保持稳定。定量分析,99mTc-HANP在第2小时在骨组织中积累。结论:粒径小于50纳米(20 ~ 31nm)的锝-99m- hanp纳米放射性药物在家兔骨组织中具有较高的吸收能力。因此,HANP可作为骨组织和骨癌的显像放射性药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Radiopharmaceutical properties of hydroxyapatite smaller than 50nm produced from eggshell and labeled with 99mTc and its biodistribution in rabbits.

Objective: Radiolabeling of nanoparticles has potential benefits for personalized treatments and theranostic applications, which have been on the agenda in recent years. Hydroxyapatite nanoparticles (HANP), which have a great similarity to bone tissue, stand out as a biocompatible nanoparticle. The different biodistribution properties of hydroxyapatite molecules in different nanosizes may create new opportunities for their use, especially in bone imaging and in the treatment of bone tumors. This study aims to investigate the labeling of hydroxyapatite molecules smaller than 50 nanometers obtained from eggshells with technetium-99m (99mTc) and the in vivo distribution of this molecule in rabbits.

Materials and methods: Characterization of nanohydroxyapatite particles obtained from eggshells was performed using scanning electron microscopy (SEM), energy dispersive X-ray (EDX), and X-ray diffraction (XRD). Radiolabeling of HANP smaller than 50nm with 99mTc radionuclide, stability of the labeled product, and biodistribution profile in rabbits were investigated.

Results: The radiochemical purity of the 99mTc-HANP was obtained as 96%. The in vitro stability of 99mTc labeled HANP was examined for up to 12 hours and showed excellent in vitro stability for the first 4 hours in saline. Technetium-99m-HANP remained stable in vivo during the 6-hour imaging period. In quantitative analysis, 99mTc-HANP showed accumulation in bone tissue in the second hour.

Conclusion: Technetium-99m-HANP nanoradiopharmaceuticals with sizes less than 50 nanometers (20-31nm) showed high uptake in bone tissue in rabbits. Therefore, HANP can be developed as imaging radiopharmaceuticals in bone tissue and bone cancers.

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来源期刊
CiteScore
1.40
自引率
6.70%
发文量
34
审稿时长
>12 weeks
期刊介绍: The Hellenic Journal of Nuclear Medicine published by the Hellenic Society of Nuclear Medicine in Thessaloniki, aims to contribute to research, to education and cover the scientific and professional interests of physicians, in the field of nuclear medicine and in medicine in general. The journal may publish papers of nuclear medicine and also papers that refer to related subjects as dosimetry, computer science, targeting of gene expression, radioimmunoassay, radiation protection, biology, cell trafficking, related historical brief reviews and other related subjects. Original papers are preferred. The journal may after special agreement publish supplements covering important subjects, dully reviewed and subscripted separately.
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