hirta对白色念珠菌的抗菌潜力初步研究。

IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES

BMC Research Notes Pub Date : 2025-04-06 DOI:10.1186/s13104-025-07209-z

Sufi Desrini

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引用次数: 0

摘要

目的：以3-（4,5-二甲基噻唑- 2-基）- 2,5-二苯基溴化四氮唑（MTT）和白色念珠菌浮游细胞为实验材料，采用微量肉汁稀释法测定白念珠菌生物膜活性，以筛选白念珠菌乙醇提取物对24 h生白念珠菌生物膜的抑制作用。结果：红桃果实乙醇提取物抑制白色念珠菌生物膜的浓度（50%）是抑制浮游细胞所需浓度的4倍（31.25µg/mL vs. 7.8µg/mL）。这一发现得到了扫描电镜下白色念珠菌生物膜形态变化的支持。使用UHPLC结合Thermo Scientific™Q Exactive™高分辨率质谱仪（HRMS）检测了55种化合物。乙醇提取物的前3位化合物：硫酸十二烷基、邻苯二甲酸二酯（2-乙基己基）和芥子酰胺可能是影响赤藓醇提物抗膜活性的主要化合物。

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The antibiofilm potential of Clidemia hirta against Candida albicans: preliminary study.

Objective: This study aims to screen the activity of ethanol extract of Clidemia hirta fruits and leaves against 24 h old Candida albicans biofilm by measuring cell viability with 3-(4,5-dimethylthiazol- 2-yl)- 2,5-diphenyl tetrazolium bromide (MTT) and planktonic cells of C. albicans using the broth microdilution method.

Results: The ethanol extract of C. hirta fruits inhibited (50%) the C. albicans biofilm at a concentration fourfold higher than those required to inhibit planktonic cells (31.25 µg/mL vs. 7.8 µg/mL). This finding is supported by the morphological changes of C. albicans biofilm under scanning electron microscopy (SEM) visualization. A total of 55 compounds were detected using UHPLC coupled with Thermo Scientific™ Q Exactive™ High-Resolution Mass Spectrometer (HRMS). The top three compounds of the ethanol extract: dodecyl sulfate, bis(2-ethylhexyl) phthalate, and erucamide) might be responsible compounds for the antibiofilm activity of ethanol extract of C. hirta.

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来源期刊

BMC Research Notes Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

3.60

自引率

0.00%

发文量

363

审稿时长

15 weeks

期刊介绍： BMC Research Notes publishes scientifically valid research outputs that cannot be considered as full research or methodology articles. We support the research community across all scientific and clinical disciplines by providing an open access forum for sharing data and useful information; this includes, but is not limited to, updates to previous work, additions to established methods, short publications, null results, research proposals and data management plans.