{"title":"四种MicroRNA表达(miR-371a-3p, miR-34c-5p, miR-146b-5p和miR-199a-3p)在突尼斯弱精子不育患者精浆中的探索","authors":"Rihab Derbel, Imen Belguith, Rim Sakka, Salima Daoud, Afifa Sellami, Hassen Kammoun, Leila Ammar Keskes","doi":"10.1155/and/8882390","DOIUrl":null,"url":null,"abstract":"<div>\n <p>Asthenozoospermia (ASZ) is a semen condition characterized by reduced sperm motility; it is observed in ~40% of male infertility cases, and it is related to various factors including genetic and epigenetic factors such as microRNAs (miRNAs). Although miRNAs play a crucial role in spermatogenesis, their function in seminal plasma and their relationship with ASZ remain largely unknown, with a considerable amount of controversial and contradictory data. This study aimed to explore the expression variation of miRNAs (miR-371a-3p, miR-34c-5p, miR-146b-5p, and miR-199a-3p) in relation to ASZ. We collected seminal plasma samples from Tunisian infertile patients with ASZ (progressive sperm motility <32%) and without ASZ (normospermic patients) and performed quantitative real-time PCR to identify differentially expressed miRNAs. Three miRNAs showed dysregulated expression in the seminal plasma of patients with ASZ (miR-34c-5p, miR-146b-5p, and miR-199a-3p). Two miRNAs were upregulated (miR-34c-5p and miR-146b-5p; <i>p</i> < 0.05), while miR-199a-3p was downregulated (<i>p</i> < 0.05) compared to patients without ASZ. The expression levels of miR-199a-3p and miR-34c-5p were significantly correlated with sperm motility and vitality percentage with an area under the receiver operating characteristic (ROC) curve (area under the curve [AUC]) of 0.75 and 0.71, respectively. These findings indicate that the three miRNAs (miR-34c-5p, miR-146b-5p, and miR-199a-3p) are implicated in the etiology of ASZ and could be recognized as potential indicators of disrupted sperm function and male infertility.</p>\n </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.1000,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/8882390","citationCount":"0","resultStr":"{\"title\":\"Exploration of Four MicroRNA Expressions (miR-371a-3p, miR-34c-5p, miR-146b-5p, and miR-199a-3p) in the Seminal Plasma of Asthenozoospermic Infertile Tunisian Patients\",\"authors\":\"Rihab Derbel, Imen Belguith, Rim Sakka, Salima Daoud, Afifa Sellami, Hassen Kammoun, Leila Ammar Keskes\",\"doi\":\"10.1155/and/8882390\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n <p>Asthenozoospermia (ASZ) is a semen condition characterized by reduced sperm motility; it is observed in ~40% of male infertility cases, and it is related to various factors including genetic and epigenetic factors such as microRNAs (miRNAs). Although miRNAs play a crucial role in spermatogenesis, their function in seminal plasma and their relationship with ASZ remain largely unknown, with a considerable amount of controversial and contradictory data. This study aimed to explore the expression variation of miRNAs (miR-371a-3p, miR-34c-5p, miR-146b-5p, and miR-199a-3p) in relation to ASZ. We collected seminal plasma samples from Tunisian infertile patients with ASZ (progressive sperm motility <32%) and without ASZ (normospermic patients) and performed quantitative real-time PCR to identify differentially expressed miRNAs. Three miRNAs showed dysregulated expression in the seminal plasma of patients with ASZ (miR-34c-5p, miR-146b-5p, and miR-199a-3p). Two miRNAs were upregulated (miR-34c-5p and miR-146b-5p; <i>p</i> < 0.05), while miR-199a-3p was downregulated (<i>p</i> < 0.05) compared to patients without ASZ. The expression levels of miR-199a-3p and miR-34c-5p were significantly correlated with sperm motility and vitality percentage with an area under the receiver operating characteristic (ROC) curve (area under the curve [AUC]) of 0.75 and 0.71, respectively. These findings indicate that the three miRNAs (miR-34c-5p, miR-146b-5p, and miR-199a-3p) are implicated in the etiology of ASZ and could be recognized as potential indicators of disrupted sperm function and male infertility.</p>\\n </div>\",\"PeriodicalId\":7817,\"journal\":{\"name\":\"Andrologia\",\"volume\":\"2025 1\",\"pages\":\"\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2025-04-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/8882390\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Andrologia\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1155/and/8882390\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ANDROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Andrologia","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1155/and/8882390","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ANDROLOGY","Score":null,"Total":0}
引用次数: 0
摘要
无精子症(ASZ)是一种以精子活力降低为特征的精液状况;约40%的男性不育症患者存在该病,该病与microRNAs (miRNAs)等遗传和表观遗传因素等多种因素有关。尽管mirna在精子发生过程中起着至关重要的作用,但其在精浆中的功能及其与ASZ的关系在很大程度上仍不清楚,有相当多的争议和矛盾的数据。本研究旨在探讨mirna (miR-371a-3p、miR-34c-5p、miR-146b-5p和miR-199a-3p)在ASZ中的表达变化。我们收集了突尼斯患有ASZ(进行性精子活力<;32%)和没有ASZ(正常精子患者)的不育患者的精液样本,并进行了定量实时PCR以鉴定差异表达的miRNAs。三种mirna在ASZ患者的精浆中表达失调(miR-34c-5p、miR-146b-5p和miR-199a-3p)。两个mirna上调(miR-34c-5p和miR-146b-5p;p & lt;miR-199a-3p下调(p <;0.05)。miR-199a-3p和miR-34c-5p的表达水平与精子活力和活力百分比显著相关,受试者工作特征(ROC)曲线下面积(area under The curve [AUC])分别为0.75和0.71。这些发现表明,三种miRNAs (miR-34c-5p, miR-146b-5p和miR-199a-3p)与ASZ的病因有关,可以被认为是精子功能破坏和男性不育的潜在指标。
Exploration of Four MicroRNA Expressions (miR-371a-3p, miR-34c-5p, miR-146b-5p, and miR-199a-3p) in the Seminal Plasma of Asthenozoospermic Infertile Tunisian Patients
Asthenozoospermia (ASZ) is a semen condition characterized by reduced sperm motility; it is observed in ~40% of male infertility cases, and it is related to various factors including genetic and epigenetic factors such as microRNAs (miRNAs). Although miRNAs play a crucial role in spermatogenesis, their function in seminal plasma and their relationship with ASZ remain largely unknown, with a considerable amount of controversial and contradictory data. This study aimed to explore the expression variation of miRNAs (miR-371a-3p, miR-34c-5p, miR-146b-5p, and miR-199a-3p) in relation to ASZ. We collected seminal plasma samples from Tunisian infertile patients with ASZ (progressive sperm motility <32%) and without ASZ (normospermic patients) and performed quantitative real-time PCR to identify differentially expressed miRNAs. Three miRNAs showed dysregulated expression in the seminal plasma of patients with ASZ (miR-34c-5p, miR-146b-5p, and miR-199a-3p). Two miRNAs were upregulated (miR-34c-5p and miR-146b-5p; p < 0.05), while miR-199a-3p was downregulated (p < 0.05) compared to patients without ASZ. The expression levels of miR-199a-3p and miR-34c-5p were significantly correlated with sperm motility and vitality percentage with an area under the receiver operating characteristic (ROC) curve (area under the curve [AUC]) of 0.75 and 0.71, respectively. These findings indicate that the three miRNAs (miR-34c-5p, miR-146b-5p, and miR-199a-3p) are implicated in the etiology of ASZ and could be recognized as potential indicators of disrupted sperm function and male infertility.
期刊介绍:
Andrologia provides an international forum for original papers on the current clinical, morphological, biochemical, and experimental status of organic male infertility and sexual disorders in men. The articles inform on the whole process of advances in andrology (including the aging male), from fundamental research to therapeutic developments worldwide. First published in 1969 and the first international journal of andrology, it is a well established journal in this expanding area of reproductive medicine.