Yimin You , Shitong Li , Hongxing Wang , Liran Wang , Luping Jiang , Xiao Li , Yanhui Peng , Zhongyi Pang , Xiyang Zhao
{"title":"尿素和复混肥对杨树根际土壤的影响机理比较","authors":"Yimin You , Shitong Li , Hongxing Wang , Liran Wang , Luping Jiang , Xiao Li , Yanhui Peng , Zhongyi Pang , Xiyang Zhao","doi":"10.1016/j.indcrop.2025.120995","DOIUrl":null,"url":null,"abstract":"<div><div>The intensive management of poplar plantation depended on nitrogen fertilizer to ensure rapid growth. It is a key prerequisite to optimize the application strategy of nitrogen fertilizer in plantation to clarify the biological mechanism difference of rhizosphere nutrient cycling driven by two nitrogen sources. Based on this, elemental analysis, high-throughput sequencing, transcriptome and metabolome were used to characterize the response of microbiome and metabolic function in poplar and rhizosphere soil to urea and compound fertilizers. The results showed that urea application significantly elevated ammonium (NH<sub>4</sub><sup>+</sup>) and soil organic carbon (SOC) content, enhancing urease and cellobiase activities to intensify carbon-nitrogen coupling. These conditions enriched <em>Proteobacteria</em>, likely through substrate-driven selection favoring taxa with nitrogen assimilation and cellulose degradation capabilities. In contrast, compound fertilizer prioritized nitrate (NO<sub>3</sub><sup>−</sup>) accumulation and stimulated acid phosphatase activity, promoting phosphorus mobilization and enriching <em>Actinobacteria</em>. Both fertilizers increased microbial α-diversity and induced β-diversity divergence. Functional profiling revealed that urea promoted cysteine metabolism and re-established galactose metabolism in roots. Compound fertilizer down-regulated the auxin catabolism gene and promoted the accumulation of auxin in roots. These findings demonstrated that urea and compound fertilizer created distinct microbial functional networks. Urea optimizes rapid nitrogen-carbon turnover, whereas compound fertilizer enhances phosphorus bioavailability. This providing a mechanistic basis for fertilizer-specific management in poplar plantations.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"229 ","pages":"Article 120995"},"PeriodicalIF":6.2000,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Contrasting mechanisms of poplar and rhizosphere soil influenced by urea and compound fertilizer\",\"authors\":\"Yimin You , Shitong Li , Hongxing Wang , Liran Wang , Luping Jiang , Xiao Li , Yanhui Peng , Zhongyi Pang , Xiyang Zhao\",\"doi\":\"10.1016/j.indcrop.2025.120995\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The intensive management of poplar plantation depended on nitrogen fertilizer to ensure rapid growth. It is a key prerequisite to optimize the application strategy of nitrogen fertilizer in plantation to clarify the biological mechanism difference of rhizosphere nutrient cycling driven by two nitrogen sources. Based on this, elemental analysis, high-throughput sequencing, transcriptome and metabolome were used to characterize the response of microbiome and metabolic function in poplar and rhizosphere soil to urea and compound fertilizers. The results showed that urea application significantly elevated ammonium (NH<sub>4</sub><sup>+</sup>) and soil organic carbon (SOC) content, enhancing urease and cellobiase activities to intensify carbon-nitrogen coupling. These conditions enriched <em>Proteobacteria</em>, likely through substrate-driven selection favoring taxa with nitrogen assimilation and cellulose degradation capabilities. In contrast, compound fertilizer prioritized nitrate (NO<sub>3</sub><sup>−</sup>) accumulation and stimulated acid phosphatase activity, promoting phosphorus mobilization and enriching <em>Actinobacteria</em>. Both fertilizers increased microbial α-diversity and induced β-diversity divergence. Functional profiling revealed that urea promoted cysteine metabolism and re-established galactose metabolism in roots. Compound fertilizer down-regulated the auxin catabolism gene and promoted the accumulation of auxin in roots. These findings demonstrated that urea and compound fertilizer created distinct microbial functional networks. Urea optimizes rapid nitrogen-carbon turnover, whereas compound fertilizer enhances phosphorus bioavailability. This providing a mechanistic basis for fertilizer-specific management in poplar plantations.</div></div>\",\"PeriodicalId\":13581,\"journal\":{\"name\":\"Industrial Crops and Products\",\"volume\":\"229 \",\"pages\":\"Article 120995\"},\"PeriodicalIF\":6.2000,\"publicationDate\":\"2025-04-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Industrial Crops and Products\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0926669025005412\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURAL ENGINEERING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Industrial Crops and Products","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0926669025005412","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURAL ENGINEERING","Score":null,"Total":0}
Contrasting mechanisms of poplar and rhizosphere soil influenced by urea and compound fertilizer
The intensive management of poplar plantation depended on nitrogen fertilizer to ensure rapid growth. It is a key prerequisite to optimize the application strategy of nitrogen fertilizer in plantation to clarify the biological mechanism difference of rhizosphere nutrient cycling driven by two nitrogen sources. Based on this, elemental analysis, high-throughput sequencing, transcriptome and metabolome were used to characterize the response of microbiome and metabolic function in poplar and rhizosphere soil to urea and compound fertilizers. The results showed that urea application significantly elevated ammonium (NH4+) and soil organic carbon (SOC) content, enhancing urease and cellobiase activities to intensify carbon-nitrogen coupling. These conditions enriched Proteobacteria, likely through substrate-driven selection favoring taxa with nitrogen assimilation and cellulose degradation capabilities. In contrast, compound fertilizer prioritized nitrate (NO3−) accumulation and stimulated acid phosphatase activity, promoting phosphorus mobilization and enriching Actinobacteria. Both fertilizers increased microbial α-diversity and induced β-diversity divergence. Functional profiling revealed that urea promoted cysteine metabolism and re-established galactose metabolism in roots. Compound fertilizer down-regulated the auxin catabolism gene and promoted the accumulation of auxin in roots. These findings demonstrated that urea and compound fertilizer created distinct microbial functional networks. Urea optimizes rapid nitrogen-carbon turnover, whereas compound fertilizer enhances phosphorus bioavailability. This providing a mechanistic basis for fertilizer-specific management in poplar plantations.
期刊介绍:
Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.