五水硼砂作为一种有前途的硼基血管生成抑制剂

IF 3.6 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Trace Elements in Medicine and Biology Pub Date : 2025-04-03 DOI:10.1016/j.jtemb.2025.127640

G. Ulus , EN Özbek , H. Yılmaz , E. Keselik , M. Sarıcaoğlu , S. Akyol Bahçeci , E. İşel , B. Debeleç Bütüner , G. Yetik Anacak , AT Koparal

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引用次数: 0

摘要

硼是一种微量元素，参与多种生理和代谢过程，最近的研究表明，硼化合物可能具有预防和治疗癌症的潜力。本研究研究了硼化合物五水硼砂（BPH）的抗血管生成作用。血管生成是一个严格调控的生物过程，负责从现有的血管形成新的血管。这一过程在癌症进展中起着关键作用，使其成为癌症治疗的重要靶点。胰腺癌和肾癌很难治疗，因为它们具有侵袭性，对化疗有抗药性。方法采用MTT法测定其抗增殖活性，并通过体外成管法和蛋鸡绒毛尿囊膜（CAM）法测定其抗血管生成作用。在HUVEC、ACHN、PANC-1细胞中采用Western blot分析BPH对VEGF水平的影响。采用体内Ehrlich腹水癌模型（EAC）研究BPH对肿瘤血管生成的影响。结果bph具有较强的抗增殖和抗血管生成活性，抑制ACHN、PANC-1和HUVECs的增殖，破坏内皮管的形成，抑制CAM表面血管的形成，并呈剂量依赖性。ACHN、PANC-1和huvec中VEGF水平明显降低。bph治疗组的VEGF和TGF-β1水平也有所下降。此外，前列腺增生引起肿瘤大小减小。结论BPH可能是一种新的抗血管生成和抗肿瘤药物。BPH作为一种有前景的新型治疗药物，可能有助于针对血管生成相关疾病的药物开发研究。

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Borax pentahydrate as a promising boron-based angiogenesis inhibitor

Background

Boron, a trace element, is involved in various physiological and metabolic processes, and recent studies suggest that boron compounds may have potential in cancer prevention and treatment. In this study, the antiangiogenic effects of a boron compound, borax pentahydrate (BPH), were investigated. Angiogenesis is a tightly regulated biological process responsible for the formation of new blood vessels from existing vasculatures. This process plays a critical role in cancer progression, making it an important target for cancer therapy. Pancreatic and kidney cancers are difficult to treat because they are aggressive and resistant to chemotherapy.

Methods

The antiproliferative activity was evaluated using the MTT assay, while antiangiogenic effects were tested through in vitro tube formation assays and in ovo chick chorioallantoic membrane (CAM) assay. The effect of BPH on VEGF levels was determined using Western blot analysis in HUVEC, ACHN, PANC-1 cells. The effect of BPH on tumor angiogenesis was investigated with an in vivo Ehrlich ascites carcinoma model (EAC).

Results

BPH exhibited potent antiproliferative and antiangiogenic activities, inhibiting the proliferation of ACHN, PANC-1, and HUVECs, disrupting endothelial tube formation, and inhibiting vascular formation on the CAM surface in a dose-dependent manner. VEGF levels were significantly decreased in ACHN, PANC-1 and HUVECs. There was also a decrease in VEGF and TGF-β1 levels in BPH-treated tumor groups. In addition, BPH caused a decrease in tumor size.

Conclusion

These findings suggest that BPH may be a new antiangiogenic and antitumoral agent. BPH may contribute to drug development studies targeting angiogenesis-related diseases as a promising new therapeutic agent.

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来源期刊

Journal of Trace Elements in Medicine and Biology 医学-内分泌学与代谢

CiteScore

6.60

自引率

2.90%

发文量

202

审稿时长

85 days

期刊介绍： The journal provides the reader with a thorough description of theoretical and applied aspects of trace elements in medicine and biology and is devoted to the advancement of scientific knowledge about trace elements and trace element species. Trace elements play essential roles in the maintenance of physiological processes. During the last decades there has been a great deal of scientific investigation about the function and binding of trace elements. The Journal of Trace Elements in Medicine and Biology focuses on the description and dissemination of scientific results concerning the role of trace elements with respect to their mode of action in health and disease and nutritional importance. Progress in the knowledge of the biological role of trace elements depends, however, on advances in trace elements chemistry. Thus the Journal of Trace Elements in Medicine and Biology will include only those papers that base their results on proven analytical methods. Also, we only publish those articles in which the quality assurance regarding the execution of experiments and achievement of results is guaranteed.