肿瘤诱导的间充质干细胞对不同类型肿瘤细胞生长发育的影响

IF 4.7 3区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL

Archives of Medical Research Pub Date : 2025-04-09 DOI:10.1016/j.arcmed.2025.103212

Mashael S. Altoub , Amal S. Alhamid , Badr Aldahmash , Ahmed Rady , Abdel Halim Harrath , Ahmed HK. El-Hashash , Pathalam Ganesan , Savarimuthu Ignacimuthu

{"title":"肿瘤诱导的间充质干细胞对不同类型肿瘤细胞生长发育的影响","authors":"Mashael S. Altoub , Amal S. Alhamid , Badr Aldahmash , Ahmed Rady , Abdel Halim Harrath , Ahmed HK. El-Hashash , Pathalam Ganesan , Savarimuthu Ignacimuthu","doi":"10.1016/j.arcmed.2025.103212","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Mesenchymal stem cells (MSCs) are present in almost all tissue types. They have multipotent properties and unique abilities to self-renew and differentiate into various cell types. We evaluated the effect of MSCs pretreated with tumor factors on three different types of common, difficult to detect cancers using several cell-based assays such as cell viability and proliferation assay, scratch assays, migration assays, and <em>in vivo</em> assays.</div></div><div><h3>Methods</h3><div>We co-cultured each type of these common cancers with either control MSCs (control TERT-GFP cells) or MSCs that were pretreated with tumor factors (conditioned medium [CM]- induced TERT-GFP cells) and compared changes in cell morphology and growth, cell proliferation, cell migration, and colony formation in an <em>in vitro</em> model. Moreover, we examined the changes in these cancers after injection with control TERT-GFP cells (untreated) and CM- induced TERT-GFP cells in an <em>in vivo</em> model.</div></div><div><h3>Results</h3><div>We detected an increase in cell proliferation of each cancer cell line when co-cultured with normal MCSs. Only HT-29 colorectal adenocarcinoma cells co-cultured with CM-induced TERT-GFP cells showed a significant reduction in cell proliferation. We observed that pre-exposure of MSCs to tumor factors decreased the attraction/migration of MSCs towards all studied tumor cell types, particularly HT-29 cells. MSCs pre-exposed to tumor factors that were co-cultured with tumor cells significantly influenced the wound healing of these cells and tumor factors markedly decreased colony formation in colorectal cancer cells.</div></div><div><h3>Conclusions</h3><div>Our results suggest that coculture of MSCs with tumor factors for 1 wk can suppress cancer cells, especially colorectal cancer cells.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 5","pages":""},"PeriodicalIF":4.7000,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effectiveness of Tumor-Induced Mesenchymal Stem Cells on Cell Growth and Development in Different Cancer Types In Vitro and In Vivo Models\",\"authors\":\"Mashael S. Altoub , Amal S. Alhamid , Badr Aldahmash , Ahmed Rady , Abdel Halim Harrath , Ahmed HK. El-Hashash , Pathalam Ganesan , Savarimuthu Ignacimuthu\",\"doi\":\"10.1016/j.arcmed.2025.103212\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>Mesenchymal stem cells (MSCs) are present in almost all tissue types. They have multipotent properties and unique abilities to self-renew and differentiate into various cell types. We evaluated the effect of MSCs pretreated with tumor factors on three different types of common, difficult to detect cancers using several cell-based assays such as cell viability and proliferation assay, scratch assays, migration assays, and <em>in vivo</em> assays.</div></div><div><h3>Methods</h3><div>We co-cultured each type of these common cancers with either control MSCs (control TERT-GFP cells) or MSCs that were pretreated with tumor factors (conditioned medium [CM]- induced TERT-GFP cells) and compared changes in cell morphology and growth, cell proliferation, cell migration, and colony formation in an <em>in vitro</em> model. Moreover, we examined the changes in these cancers after injection with control TERT-GFP cells (untreated) and CM- induced TERT-GFP cells in an <em>in vivo</em> model.</div></div><div><h3>Results</h3><div>We detected an increase in cell proliferation of each cancer cell line when co-cultured with normal MCSs. Only HT-29 colorectal adenocarcinoma cells co-cultured with CM-induced TERT-GFP cells showed a significant reduction in cell proliferation. We observed that pre-exposure of MSCs to tumor factors decreased the attraction/migration of MSCs towards all studied tumor cell types, particularly HT-29 cells. MSCs pre-exposed to tumor factors that were co-cultured with tumor cells significantly influenced the wound healing of these cells and tumor factors markedly decreased colony formation in colorectal cancer cells.</div></div><div><h3>Conclusions</h3><div>Our results suggest that coculture of MSCs with tumor factors for 1 wk can suppress cancer cells, especially colorectal cancer cells.</div></div>\",\"PeriodicalId\":8318,\"journal\":{\"name\":\"Archives of Medical Research\",\"volume\":\"56 5\",\"pages\":\"\"},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2025-04-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archives of Medical Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0188440925000323\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Medical Research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0188440925000323","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}

引用次数: 0

摘要

背景：间充质干细胞几乎存在于所有组织类型中。间充质干细胞具有多能特性和自我更新及分化成各种细胞类型的独特能力。我们使用多种基于细胞的试验，如细胞活力和增殖试验、划痕试验、迁移试验和体内试验，评估了间充质干细胞经肿瘤因子预处理后对三种不同类型的常见、难以检测的癌症的影响：我们将每种常见癌症与对照间充质干细胞（对照TERT-GFP细胞）或经肿瘤因子预处理的间充质干细胞（条件培养基[CM]诱导的TERT-GFP细胞）共同培养，并在体外模型中比较细胞形态和生长、细胞增殖、细胞迁移和集落形成的变化。此外，我们还在体内模型中检测了注射对照 TERT-GFP 细胞（未处理）和 CM 诱导的 TERT-GFP 细胞后这些癌症的变化：结果：我们检测到，与正常多囊卵巢综合征细胞共同培养时，每种癌细胞系的细胞增殖都有所增加。只有 HT-29 大肠腺癌细胞与 CM 诱导的 TERT-GFP 细胞共同培养后，细胞增殖明显减少。我们观察到，间充质干细胞预先暴露于肿瘤因子会降低间充质干细胞对所有研究的肿瘤细胞类型（尤其是 HT-29 细胞）的吸引/迁移。预先暴露于肿瘤因子的间充质干细胞与肿瘤细胞共培养会显著影响这些细胞的伤口愈合，肿瘤因子会明显减少结直肠癌细胞的集落形成：我们的研究结果表明，间充质干细胞与肿瘤因子共培养 1 周可抑制癌细胞，尤其是结直肠癌细胞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Effectiveness of Tumor-Induced Mesenchymal Stem Cells on Cell Growth and Development in Different Cancer Types In Vitro and In Vivo Models

Background

Mesenchymal stem cells (MSCs) are present in almost all tissue types. They have multipotent properties and unique abilities to self-renew and differentiate into various cell types. We evaluated the effect of MSCs pretreated with tumor factors on three different types of common, difficult to detect cancers using several cell-based assays such as cell viability and proliferation assay, scratch assays, migration assays, and in vivo assays.

Methods

We co-cultured each type of these common cancers with either control MSCs (control TERT-GFP cells) or MSCs that were pretreated with tumor factors (conditioned medium [CM]- induced TERT-GFP cells) and compared changes in cell morphology and growth, cell proliferation, cell migration, and colony formation in an in vitro model. Moreover, we examined the changes in these cancers after injection with control TERT-GFP cells (untreated) and CM- induced TERT-GFP cells in an in vivo model.

Results

We detected an increase in cell proliferation of each cancer cell line when co-cultured with normal MCSs. Only HT-29 colorectal adenocarcinoma cells co-cultured with CM-induced TERT-GFP cells showed a significant reduction in cell proliferation. We observed that pre-exposure of MSCs to tumor factors decreased the attraction/migration of MSCs towards all studied tumor cell types, particularly HT-29 cells. MSCs pre-exposed to tumor factors that were co-cultured with tumor cells significantly influenced the wound healing of these cells and tumor factors markedly decreased colony formation in colorectal cancer cells.

Conclusions

Our results suggest that coculture of MSCs with tumor factors for 1 wk can suppress cancer cells, especially colorectal cancer cells.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Archives of Medical Research 医学-医学：研究与实验

CiteScore

12.50

自引率

0.00%

发文量

审稿时长

28 days

期刊介绍： Archives of Medical Research serves as a platform for publishing original peer-reviewed medical research, aiming to bridge gaps created by medical specialization. The journal covers three main categories - biomedical, clinical, and epidemiological contributions, along with review articles and preliminary communications. With an international scope, it presents the study of diseases from diverse perspectives, offering the medical community original investigations ranging from molecular biology to clinical epidemiology in a single publication.