Carlos E Almeida, Lifeng Du, Jingwen Wang, Dayane Pires-Silva, Elaine Folly-Ramos, Myrian Harry, Cleber Galvão
{"title":"巴西东北部半干旱地区主要南美锥虫病病媒巴西南美锥虫（Triatoma brasiliensis brasiliensis）（半翅目：Reduviidae）的有丝分裂基因组。","authors":"Carlos E Almeida, Lifeng Du, Jingwen Wang, Dayane Pires-Silva, Elaine Folly-Ramos, Myrian Harry, Cleber Galvão","doi":"10.1186/s13071-025-06769-0","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Triatoma brasiliensis brasiliensis is the primary vector of Chagas disease in Brazil's semi-arid regions, exhibiting adaptability to various environments, including domestic and peridomestic. Despite its significance, comprehensive genomic data for this subspecies remain limited.</p><p><strong>Methods: </strong>We assembled the complete mitochondrial genome of T. b. brasiliensis using a combination of Illumina and Sanger sequencing technologies, the latter being necessary to obtain the control region with eight primers designed in this study. The mitogenome was annotated to identify gene content and organization. Phylogenetic relationships were inferred using conserved blocks of 13 protein-coding genes and 22 transfer RNA genes. For this analysis, 18 representative triatomines with near-complete mitogenomes were selected, and phylogenetic reconstruction was performed using the maximum ikelihood method.</p><p><strong>Results: </strong>The complete mitogenome spans 16,575 base pairs and includes 13 protein-coding genes, 22 transfer RNA genes, and two ribosomal RNA genes, consistent with the typical structure of insect mitochondrial genomes. The control region exhibited tandem and inverted repeats arranged in blocks, as observed for other Reduviidae. Given the limited availability of mitogenomes, our phylogenetic analysis provided statistical support for T. b. brasiliensis as a sister taxon to Triatoma infestans, forming a well-supported clade that is sister to Triatoma vitticeps.</p><p><strong>Conclusions: </strong>The availability of this mitogenome provides insights into the systematics, biology, and genomics of triatomine species while also enhancing our understanding of their evolutionary relationships. However, the limited number of available mitogenomes, particularly for South American Triatoma species, underscores the need for further sequencing efforts to improve phylogenetic resolution and support comparative genomic studies.</p>","PeriodicalId":19793,"journal":{"name":"Parasites & Vectors","volume":"18 1","pages":"131"},"PeriodicalIF":3.0000,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969816/pdf/","citationCount":"0","resultStr":"{\"title\":\"The mitogenome of Triatoma brasiliensis brasiliensis (Hemiptera: Reduviidae), the main Chagas disease vector in the semi-arid region of northeastern Brazil.\",\"authors\":\"Carlos E Almeida, Lifeng Du, Jingwen Wang, Dayane Pires-Silva, Elaine Folly-Ramos, Myrian Harry, Cleber Galvão\",\"doi\":\"10.1186/s13071-025-06769-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Triatoma brasiliensis brasiliensis is the primary vector of Chagas disease in Brazil's semi-arid regions, exhibiting adaptability to various environments, including domestic and peridomestic. Despite its significance, comprehensive genomic data for this subspecies remain limited.</p><p><strong>Methods: </strong>We assembled the complete mitochondrial genome of T. b. brasiliensis using a combination of Illumina and Sanger sequencing technologies, the latter being necessary to obtain the control region with eight primers designed in this study. The mitogenome was annotated to identify gene content and organization. Phylogenetic relationships were inferred using conserved blocks of 13 protein-coding genes and 22 transfer RNA genes. For this analysis, 18 representative triatomines with near-complete mitogenomes were selected, and phylogenetic reconstruction was performed using the maximum ikelihood method.</p><p><strong>Results: </strong>The complete mitogenome spans 16,575 base pairs and includes 13 protein-coding genes, 22 transfer RNA genes, and two ribosomal RNA genes, consistent with the typical structure of insect mitochondrial genomes. The control region exhibited tandem and inverted repeats arranged in blocks, as observed for other Reduviidae. Given the limited availability of mitogenomes, our phylogenetic analysis provided statistical support for T. b. brasiliensis as a sister taxon to Triatoma infestans, forming a well-supported clade that is sister to Triatoma vitticeps.</p><p><strong>Conclusions: </strong>The availability of this mitogenome provides insights into the systematics, biology, and genomics of triatomine species while also enhancing our understanding of their evolutionary relationships. However, the limited number of available mitogenomes, particularly for South American Triatoma species, underscores the need for further sequencing efforts to improve phylogenetic resolution and support comparative genomic studies.</p>\",\"PeriodicalId\":19793,\"journal\":{\"name\":\"Parasites & Vectors\",\"volume\":\"18 1\",\"pages\":\"131\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2025-04-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969816/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Parasites & Vectors\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1186/s13071-025-06769-0\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Parasites & Vectors","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s13071-025-06769-0","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PARASITOLOGY","Score":null,"Total":0}
The mitogenome of Triatoma brasiliensis brasiliensis (Hemiptera: Reduviidae), the main Chagas disease vector in the semi-arid region of northeastern Brazil.
Background: Triatoma brasiliensis brasiliensis is the primary vector of Chagas disease in Brazil's semi-arid regions, exhibiting adaptability to various environments, including domestic and peridomestic. Despite its significance, comprehensive genomic data for this subspecies remain limited.
Methods: We assembled the complete mitochondrial genome of T. b. brasiliensis using a combination of Illumina and Sanger sequencing technologies, the latter being necessary to obtain the control region with eight primers designed in this study. The mitogenome was annotated to identify gene content and organization. Phylogenetic relationships were inferred using conserved blocks of 13 protein-coding genes and 22 transfer RNA genes. For this analysis, 18 representative triatomines with near-complete mitogenomes were selected, and phylogenetic reconstruction was performed using the maximum ikelihood method.
Results: The complete mitogenome spans 16,575 base pairs and includes 13 protein-coding genes, 22 transfer RNA genes, and two ribosomal RNA genes, consistent with the typical structure of insect mitochondrial genomes. The control region exhibited tandem and inverted repeats arranged in blocks, as observed for other Reduviidae. Given the limited availability of mitogenomes, our phylogenetic analysis provided statistical support for T. b. brasiliensis as a sister taxon to Triatoma infestans, forming a well-supported clade that is sister to Triatoma vitticeps.
Conclusions: The availability of this mitogenome provides insights into the systematics, biology, and genomics of triatomine species while also enhancing our understanding of their evolutionary relationships. However, the limited number of available mitogenomes, particularly for South American Triatoma species, underscores the need for further sequencing efforts to improve phylogenetic resolution and support comparative genomic studies.
期刊介绍：
Parasites & Vectors is an open access, peer-reviewed online journal dealing with the biology of parasites, parasitic diseases, intermediate hosts, vectors and vector-borne pathogens. Manuscripts published in this journal will be available to all worldwide, with no barriers to access, immediately following acceptance. However, authors retain the copyright of their material and may use it, or distribute it, as they wish.
Manuscripts on all aspects of the basic and applied biology of parasites, intermediate hosts, vectors and vector-borne pathogens will be considered. In addition to the traditional and well-established areas of science in these fields, we also aim to provide a vehicle for publication of the rapidly developing resources and technology in parasite, intermediate host and vector genomics and their impacts on biological research. We are able to publish large datasets and extensive results, frequently associated with genomic and post-genomic technologies, which are not readily accommodated in traditional journals. Manuscripts addressing broader issues, for example economics, social sciences and global climate change in relation to parasites, vectors and disease control, are also welcomed.
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