酵母Hsp78通过轻度乙醇胁迫预处理在线粒体蛋白质量控制中对严重乙醇胁迫的适应中起重要作用。

IF 2.8 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Fuko Horie, Ryoko Ando, Koharu Sekimoto, Vo Thi Anh Nguyet, Shingo Izawa
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引用次数: 0

摘要

严重的乙醇胁迫(10 % v/v)会导致某些线粒体蛋白(如乌头酶(Aco1))变性和聚集,在出芽酵母中形成未折叠线粒体蛋白沉淀(DUMPs)。让酵母细胞预先承受轻微应激往往会诱导其适应随后的严重应激。然而,让酵母细胞预先暴露于轻度乙醇胁迫是否能减轻线粒体蛋白聚集仍不清楚。因此,在本研究中,我们考察了在严重乙醇胁迫下,酵母细胞预暴露于轻度乙醇胁迫对酵母线粒体蛋白质量控制系统(mtPQC)的影响。在野生型细胞中,6%(v/v)乙醇预处理能显著缓解随后 10%乙醇胁迫下 DUMPs 和 Aco1 聚集体的形成,但在 hsp78∆ 和 mdj1∆ 细胞中却不能。6%乙醇预处理增加了mtPQC相关因子、Hsp78、Mdj1和Hsp10的蛋白水平;然而,hsp78∆细胞中Ssc1(mtHsp70)及其协同伴侣Mdj1的水平明显低于野生型细胞。此外,野生型细胞经 6% 乙醇预处理后,细胞内活性氧水平和 10% 乙醇胁迫下呼吸缺陷突变体的频率降低,而 hsp78∆ 细胞则没有降低。总之,这项研究证明,酵母细胞预先暴露于轻度乙醇胁迫可通过激活 mtPQC 系统(包括 HSP78 的表达)减轻乙醇诱导的线粒体损伤,从而为乙醇胁迫对线粒体的影响以及酵母的相应反应提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Yeast Hsp78 plays an essential role in adapting to severe ethanol stress via mild ethanol stress pretreatment in mitochondrial protein quality control
Severe ethanol stress (10 % v/v) causes the denaturation and aggregation of certain mitochondrial proteins, such as aconitase (Aco1), forming the deposits of unfolded mitochondrial proteins (DUMPs) in the budding yeast Saccharomyces cerevisiae. Pre-exposing yeast cells to mild stress often induces adaptation to subsequent severe stress. However, whether pre-exposing yeast cells to mild ethanol stress mitigates mitochondrial protein aggregation remains unclear. Therefore, in this study, we examined the effects of pre-exposing yeast cells to mild ethanol stress on the yeast mitochondrial protein quality control (mtPQC) system under severe ethanol stress. Pretreatment with 6 % (v/v) ethanol significantly mitigated the formation of DUMPs and Aco1 aggregates under subsequent 10 % ethanol stress in wild-type cells but not in hsp78∆ and mdj1∆ cells. Pretreatment with 6 % ethanol increased the protein levels of mtPQC-related factors, Hsp78, Mdj1, and Hsp10; however, hsp78∆ cells showed significantly lower levels of Ssc1 (mtHsp70) and its co-chaperone Mdj1 than wild-type cells. Moreover, intracellular reactive oxygen species levels and the frequency of respiration-deficient mutants under 10 % ethanol stress were reduced after pretreatment with 6 % ethanol in wild-type cells but not in hsp78∆ cells. Overall, this study demonstrated that pre-exposing yeast cells to mild ethanol stress mitigated ethanol-induced mitochondrial damage by activating the mtPQC system, including HSP78 expression, providing novel insights into the effects of ethanol stress on mitochondria and the corresponding responses in yeast.
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来源期刊
Biochimica et biophysica acta. General subjects
Biochimica et biophysica acta. General subjects 生物-生化与分子生物学
CiteScore
6.40
自引率
0.00%
发文量
139
审稿时长
30 days
期刊介绍: BBA General Subjects accepts for submission either original, hypothesis-driven studies or reviews covering subjects in biochemistry and biophysics that are considered to have general interest for a wide audience. Manuscripts with interdisciplinary approaches are especially encouraged.
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