无标记定量赖氨酸乳酸蛋白组分析方案。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2025-04-03 DOI:10.1016/j.xpro.2025.103726

Yu Ran, Qiqing Yang, Lianqi Zhou, Heyu Li, Bing Yang, Long Zhang

{"title":"无标记定量赖氨酸乳酸蛋白组分析方案。","authors":"Yu Ran, Qiqing Yang, Lianqi Zhou, Heyu Li, Bing Yang, Long Zhang","doi":"10.1016/j.xpro.2025.103726","DOIUrl":null,"url":null,"abstract":"L-lactate has been recognized as an essential molecule for signaling and metabolic balance. Lactylation, a post-translational modification (PTM) derived from L-lactate, is commonly observed on various proteins and plays essential roles in cellular processes. Here, we present a protocol to globally profile the lactylation proteome and perform label-free quantification. We provide steps for cell preparation, protein extraction, digestion, peptide desalting, and lactylpeptide enrichment. Additionally, we outline the parameters for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. For complete details on the use and execution of this protocol, please refer to Li et al.1.","PeriodicalId":34214,"journal":{"name":"STAR Protocols","volume":"6 2","pages":"103726"},"PeriodicalIF":1.3000,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11999609/pdf/","citationCount":"0","resultStr":"{\"title\":\"Protocol for label-free quantitative lysine lactylproteome profiling.\",\"authors\":\"Yu Ran, Qiqing Yang, Lianqi Zhou, Heyu Li, Bing Yang, Long Zhang\",\"doi\":\"10.1016/j.xpro.2025.103726\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"L-lactate has been recognized as an essential molecule for signaling and metabolic balance. Lactylation, a post-translational modification (PTM) derived from L-lactate, is commonly observed on various proteins and plays essential roles in cellular processes. Here, we present a protocol to globally profile the lactylation proteome and perform label-free quantification. We provide steps for cell preparation, protein extraction, digestion, peptide desalting, and lactylpeptide enrichment. Additionally, we outline the parameters for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. For complete details on the use and execution of this protocol, please refer to Li et al.1.\",\"PeriodicalId\":34214,\"journal\":{\"name\":\"STAR Protocols\",\"volume\":\"6 2\",\"pages\":\"103726\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2025-04-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11999609/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"STAR Protocols\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1016/j.xpro.2025.103726\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"STAR Protocols","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.xpro.2025.103726","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

摘要

l -乳酸被认为是信号传导和代谢平衡的重要分子。乳酸化是一种由l -乳酸盐衍生的翻译后修饰（PTM），在多种蛋白质上普遍存在，在细胞过程中起着重要作用。在这里，我们提出了一种方案来全局分析乳酸化蛋白质组，并进行无标记量化。我们提供细胞制备，蛋白质提取，消化，肽脱盐和乳酸肽富集的步骤。此外，我们概述了液相色谱-串联质谱（LC-MS/MS）分析的参数。有关该协议的使用和执行的完整细节，请参阅Li等人1。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Protocol for label-free quantitative lysine lactylproteome profiling.

L-lactate has been recognized as an essential molecule for signaling and metabolic balance. Lactylation, a post-translational modification (PTM) derived from L-lactate, is commonly observed on various proteins and plays essential roles in cellular processes. Here, we present a protocol to globally profile the lactylation proteome and perform label-free quantification. We provide steps for cell preparation, protein extraction, digestion, peptide desalting, and lactylpeptide enrichment. Additionally, we outline the parameters for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. For complete details on the use and execution of this protocol, please refer to Li et al.¹.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊